ID H0TTI8_9BRAD Unreviewed; 542 AA.
AC H0TTI8;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 24-JAN-2024, entry version 43.
DE SubName: Full=Choline dehydrogenase, a flavoprotein alcohol dehydrogenase {ECO:0000313|EMBL:CCE09776.1};
DE EC=1.1.99.1 {ECO:0000313|EMBL:CCE09776.1};
GN Name=betA {ECO:0000313|EMBL:CCE09776.1};
GN ORFNames=BRAS3843_3330028 {ECO:0000313|EMBL:CCE09776.1};
OS Bradyrhizobium sp. STM 3843.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Nitrobacteraceae; Bradyrhizobium.
OX NCBI_TaxID=551947 {ECO:0000313|EMBL:CCE09776.1, ECO:0000313|Proteomes:UP000002686};
RN [1] {ECO:0000313|Proteomes:UP000002686}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=STM3843 {ECO:0000313|Proteomes:UP000002686};
RX PubMed=24704842; DOI=10.3390/genes3010035;
RA Mornico D., Miche L., Bena G., Nouwen N., Vermeglio A., Vallenet D.,
RA Smith A.A., Giraud E., Medigue C., Moulin L.;
RT "Comparative genomics of aeschynomene symbionts: insights into the
RT ecological lifestyle of nod-independent photosynthetic bradyrhizobia.";
RL Genes (Basel) 3:35-61(2011).
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974,
CC ECO:0000256|PIRSR:PIRSR000137-2};
CC -!- SIMILARITY: Belongs to the GMC oxidoreductase family.
CC {ECO:0000256|ARBA:ARBA00010790, ECO:0000256|RuleBase:RU003968}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCE09776.1}.
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DR EMBL; CAFK01000261; CCE09776.1; -; Genomic_DNA.
DR RefSeq; WP_008972287.1; NZ_CAFK01000261.1.
DR AlphaFoldDB; H0TTI8; -.
DR OrthoDB; 9785276at2; -.
DR Proteomes; UP000002686; Unassembled WGS sequence.
DR GO; GO:0008812; F:choline dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR Gene3D; 3.30.560.10; Glucose Oxidase, domain 3; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR012132; GMC_OxRdtase.
DR InterPro; IPR000172; GMC_OxRdtase_N.
DR InterPro; IPR007867; GMC_OxRtase_C.
DR PANTHER; PTHR11552:SF147; CHOLINE DEHYDROGENASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR11552; GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE; 1.
DR Pfam; PF05199; GMC_oxred_C; 1.
DR Pfam; PF00732; GMC_oxred_N; 1.
DR PIRSF; PIRSF000137; Alcohol_oxidase; 1.
DR SUPFAM; SSF54373; FAD-linked reductases, C-terminal domain; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR PROSITE; PS00623; GMC_OXRED_1; 1.
DR PROSITE; PS00624; GMC_OXRED_2; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|PIRSR:PIRSR000137-2};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630,
KW ECO:0000256|RuleBase:RU003968};
KW Oxidoreductase {ECO:0000313|EMBL:CCE09776.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000002686}.
FT DOMAIN 92..115
FT /note="Glucose-methanol-choline oxidoreductase N-terminal"
FT /evidence="ECO:0000259|PROSITE:PS00623"
FT DOMAIN 264..278
FT /note="Glucose-methanol-choline oxidoreductase N-terminal"
FT /evidence="ECO:0000259|PROSITE:PS00624"
FT BINDING 94
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR000137-2"
SQ SEQUENCE 542 AA; 59515 MW; 5A7687437DAFAFE8 CRC64;
MSGSRAASHE TDLEFDYVVV GAGSAGCVLA NRLSADGKHK VLLLEAGPKD SNIWIHVPLG
YGKLFKDKSV NWMYQTEPEP GLDGRSVFQP RGKVLGGSSS INGLLYVRGQ NEDYDRWRQR
GNVGWGYEDV LPYFKRAENQ QRGADDYHGA GGPLSVSDWR HEDPLSEAFV KAAVETGLPY
NPDFNGAAQE GAGFFQTTTI RGRRASSAFC YLRPAKHRSN LHIETDAQAE RIVFEGRRAR
AVEFRQHGRL RTARARREIL VSSGAYNSPQ LLQLSGIGPA ELLKRHGIDV VLDAPGVGSD
LQDHMQVRIV MRCSQRITLN DIVHHPVRRV LAGARYAAFR TGPLTIAAGT AGAFFKTDPR
LASPDIQIHF IPFSTDKMGE TLHTYSGFTA SVCQLRPESR GSLQIRSADP AAAPEIRINY
LASETDRRAN IDGLRILRKI LAAPALKPYV TDEAYPGSKV VADEEILAYC RQTGSTIYHP
TSTCRMGNDA LAVVDERLRL RGIEGLRVVD ASIMPDLVSG NTNAPVIMIA EKASDMILQD
AH
//
