ID H0VH97_CAVPO Unreviewed; 989 AA.
AC H0VH97;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 2.
DT 27-MAR-2024, entry version 80.
DE SubName: Full=Rap guanine nucleotide exchange factor 4 {ECO:0000313|Ensembl:ENSCPOP00000009523.3};
GN Name=RAPGEF4 {ECO:0000313|Ensembl:ENSCPOP00000009523.3};
OS Cavia porcellus (Guinea pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Hystricomorpha; Caviidae;
OC Cavia.
OX NCBI_TaxID=10141 {ECO:0000313|Ensembl:ENSCPOP00000009523.3, ECO:0000313|Proteomes:UP000005447};
RN [1] {ECO:0000313|Proteomes:UP000005447}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=2N {ECO:0000313|Proteomes:UP000005447};
RX PubMed=21993624; DOI=10.1038/nature10530;
RA Lindblad-Toh K., Garber M., Zuk O., Lin M.F., Parker B.J., Washietl S.,
RA Kheradpour P., Ernst J., Jordan G., Mauceli E., Ward L.D., Lowe C.B.,
RA Holloway A.K., Clamp M., Gnerre S., Alfoldi J., Beal K., Chang J.,
RA Clawson H., Cuff J., Di Palma F., Fitzgerald S., Flicek P., Guttman M.,
RA Hubisz M.J., Jaffe D.B., Jungreis I., Kent W.J., Kostka D., Lara M.,
RA Martins A.L., Massingham T., Moltke I., Raney B.J., Rasmussen M.D.,
RA Robinson J., Stark A., Vilella A.J., Wen J., Xie X., Zody M.C., Baldwin J.,
RA Bloom T., Chin C.W., Heiman D., Nicol R., Nusbaum C., Young S.,
RA Wilkinson J., Worley K.C., Kovar C.L., Muzny D.M., Gibbs R.A., Cree A.,
RA Dihn H.H., Fowler G., Jhangiani S., Joshi V., Lee S., Lewis L.R.,
RA Nazareth L.V., Okwuonu G., Santibanez J., Warren W.C., Mardis E.R.,
RA Weinstock G.M., Wilson R.K., Delehaunty K., Dooling D., Fronik C.,
RA Fulton L., Fulton B., Graves T., Minx P., Sodergren E., Birney E.,
RA Margulies E.H., Herrero J., Green E.D., Haussler D., Siepel A., Goldman N.,
RA Pollard K.S., Pedersen J.S., Lander E.S., Kellis M.;
RT "A high-resolution map of human evolutionary constraint using 29 mammals.";
RL Nature 478:476-482(2011).
RN [2] {ECO:0000313|Ensembl:ENSCPOP00000009523.3}
RP IDENTIFICATION.
RC STRAIN=2N {ECO:0000313|Ensembl:ENSCPOP00000009523.3};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
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DR EMBL; AAKN02005638; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAKN02005639; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAKN02005640; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAKN02005641; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAKN02005642; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; H0VH97; -.
DR STRING; 10141.ENSCPOP00000009523; -.
DR Ensembl; ENSCPOT00000010702.3; ENSCPOP00000009523.3; ENSCPOG00000010605.4.
DR VEuPathDB; HostDB:ENSCPOG00000010605; -.
DR eggNOG; KOG2378; Eukaryota.
DR GeneTree; ENSGT00940000156075; -.
DR HOGENOM; CLU_006829_1_0_1; -.
DR InParanoid; H0VH97; -.
DR OMA; LYCYVLE; -.
DR TreeFam; TF313184; -.
DR Proteomes; UP000005447; Unassembled WGS sequence.
DR Bgee; ENSCPOG00000010605; Expressed in adrenal gland and 13 other cell types or tissues.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0098686; C:hippocampal mossy fiber to CA3 synapse; IEA:Ensembl.
DR GO; GO:0030552; F:cAMP binding; IEA:Ensembl.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR GO; GO:0030674; F:protein-macromolecule adaptor activity; IEA:Ensembl.
DR GO; GO:0031267; F:small GTPase binding; IEA:Ensembl.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IEA:Ensembl.
DR GO; GO:0017156; P:calcium-ion regulated exocytosis; IEA:Ensembl.
DR GO; GO:0030073; P:insulin secretion; IEA:Ensembl.
DR GO; GO:0032024; P:positive regulation of insulin secretion; IEA:Ensembl.
DR GO; GO:0017157; P:regulation of exocytosis; IEA:Ensembl.
DR GO; GO:0098693; P:regulation of synaptic vesicle cycle; IEA:Ensembl.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR CDD; cd00038; CAP_ED; 2.
DR CDD; cd04437; DEP_Epac; 1.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 1.10.8.1240; -; 1.
DR Gene3D; 2.60.120.10; Jelly Rolls; 2.
DR Gene3D; 1.10.840.10; Ras guanine-nucleotide exchange factors catalytic domain; 1.
DR Gene3D; 1.20.870.10; Son of sevenless (SoS) protein Chain: S domain 1; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR018490; cNMP-bd_dom_sf.
DR InterPro; IPR000591; DEP_dom.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR019804; Ras_G-nucl-exch_fac_CS.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR23113; GUANINE NUCLEOTIDE EXCHANGE FACTOR; 1.
DR PANTHER; PTHR23113:SF175; RAP GUANINE NUCLEOTIDE EXCHANGE FACTOR 4; 1.
DR Pfam; PF00027; cNMP_binding; 2.
DR Pfam; PF00610; DEP; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR PRINTS; PR00103; CAMPKINASE.
DR SMART; SM00100; cNMP; 2.
DR SMART; SM00049; DEP; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF51206; cAMP-binding domain-like; 2.
DR SUPFAM; SSF48366; Ras GEF; 1.
DR SUPFAM; SSF54236; Ubiquitin-like; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 2.
DR PROSITE; PS50186; DEP; 1.
DR PROSITE; PS00720; RASGEF; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 4: Predicted;
KW Guanine-nucleotide releasing factor {ECO:0000256|ARBA:ARBA00022658,
KW ECO:0000256|PROSITE-ProRule:PRU00168};
KW Reference proteome {ECO:0000313|Proteomes:UP000005447}.
FT DOMAIN 21..124
FT /note="Cyclic nucleotide-binding"
FT /evidence="ECO:0000259|PROSITE:PS50042"
FT DOMAIN 194..269
FT /note="DEP"
FT /evidence="ECO:0000259|PROSITE:PS50186"
FT DOMAIN 334..435
FT /note="Cyclic nucleotide-binding"
FT /evidence="ECO:0000259|PROSITE:PS50042"
FT DOMAIN 474..612
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50212"
FT DOMAIN 750..987
FT /note="Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50009"
SQ SEQUENCE 989 AA; 113102 MW; 969488C1F1472330 CRC64;
PLERSSEDVD IIFTRLKEVK AFEKFHPNLL HQICLCGYYE NLEKGITLFR QGDIGTNWYA
VLAGSLDVKV SETSSHQDAV TICTLGIGTA FGESILDNTP RHATIVTRES SELLRIEQKD
FKALWEKYRQ YMAGLLAPPY GVMETGSNND RIPDKENTPL IEPHLPLRPA NTITKVPSEK
ILRAGKILRN AILSRAPHMI RDRKYHLKTY RQCCVGTELV DWMLQQTPCV HSRTQAVGMW
QVLLEDGVLN HVDQEHHFQD KYLFYRFLDD EHEDAPLPTE EEKKECDEEL QDTMLLLSQM
GPDAHMRMIL RKPPGQRTVD DLEIIYEELL HIKALSHLST TVKRELAGVL IFESHAKGGT
VLFNQGEEGT SWYIILKGSV NVVIYGKGVV CTLHEGDDFG KLALVNDAPR AASIVLREDN
CHFLRVDKED FNRILRDVEA NTVRLKEHDQ DVLVLEKVPA GSRASNQGNS QPQQKYTVMS
GTPEKILEHF LETIRLEPAL NEATDSILND FIMMHCVFMP NTQLCPALVA HYHAQPSQGT
EQEKLDYALN NKRRVIRLVL QWASTYGDLL QEDDVAMAFL EEFYVSVSDD ARMIAALKEQ
LPELERIVKQ ISEDAKAPQK KHKVLLQQFN TSDERAQKRQ PIRGSDEVLF KVYCMDHTYT
TIRVPVAASV KEVISAVADK LGSGEGLIIV KMSSGGEKVV LKPNDVSVFT TLTINGRLFA
CPREQFDSLT PLPEQEGPTV GTVGTFELMS SKDLAYQMTI YDWELFNCVH ELELIYHTFG
RHNFKKTTAN LDLFLRRFNE IQFWVVTEIC LCSQLSKRVQ LLKKFIKIAA HCKEYKNLNS
FFAIVMGLSN VAVSRLALTW EKLPSKFKKF YAEFESLMDP SRNHRAYRLT AAKLEPPLIP
FMPLLIKDMT FTHEGNKTFI DNLVNFEKMR MIANTARTVR YYRSQPFSPD AAQANKNHQD
VRSYVRQLNV IDNQRTLSQM SHRLEPRRP
//
