ID H0WM43_OTOGA Unreviewed; 1572 AA.
AC H0WM43;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 27-MAR-2024, entry version 70.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN Name=HECW2 {ECO:0000313|Ensembl:ENSOGAP00000002779.2};
OS Otolemur garnettii (Small-eared galago) (Garnett's greater bushbaby).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Strepsirrhini; Lorisiformes;
OC Galagidae; Otolemur.
OX NCBI_TaxID=30611 {ECO:0000313|Ensembl:ENSOGAP00000002779.2, ECO:0000313|Proteomes:UP000005225};
RN [1] {ECO:0000313|Proteomes:UP000005225}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG The Broad Institute Genome Sequencing Platform;
RA Di Palma F., Johnson J., Lander E.S., Lindblad-Toh K., Jaffe D.B.,
RA Gnerre S., MacCallum I., Przybylski D., Ribeiro F.J., Burton J.N.,
RA Walker B.J., Sharpe T., Hall G.;
RT "Version 3 of the genome sequence of Otolemur garnettii (Bushbaby).";
RL Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSOGAP00000002779.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
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DR EMBL; AAQR03125056; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03125057; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03125058; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03125059; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03125060; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03125061; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03125062; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03125063; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03125064; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03125065; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR STRING; 30611.ENSOGAP00000002779; -.
DR Ensembl; ENSOGAT00000003107.2; ENSOGAP00000002779.2; ENSOGAG00000003101.2.
DR eggNOG; KOG0940; Eukaryota.
DR GeneTree; ENSGT00940000155466; -.
DR HOGENOM; CLU_002173_14_0_1; -.
DR InParanoid; H0WM43; -.
DR OMA; TAGQHRE; -.
DR TreeFam; TF313938; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000005225; Unassembled WGS sequence.
DR GO; GO:0072686; C:mitotic spindle; IEA:Ensembl.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0030071; P:regulation of mitotic metaphase/anaphase transition; IEA:Ensembl.
DR CDD; cd08691; C2_NEDL1-like; 1.
DR CDD; cd00078; HECTc; 1.
DR CDD; cd00201; WW; 2.
DR Gene3D; 2.20.70.10; -; 2.
DR Gene3D; 2.60.40.2840; -; 1.
DR Gene3D; 2.60.40.150; C2 domain; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR037795; C2_HECW.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR040524; HECW1_helix.
DR InterPro; IPR032348; HECW_N.
DR InterPro; IPR001202; WW_dom.
DR InterPro; IPR036020; WW_dom_sf.
DR PANTHER; PTHR11254:SF127; E3 UBIQUITIN-PROTEIN LIGASE HECW2; 1.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR Pfam; PF00168; C2; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF18436; HECW1_helix; 1.
DR Pfam; PF16562; HECW_N; 1.
DR Pfam; PF00397; WW; 1.
DR SMART; SM00239; C2; 1.
DR SMART; SM00119; HECTc; 1.
DR SMART; SM00456; WW; 2.
DR SUPFAM; SSF49562; C2 domain (Calcium/lipid-binding domain, CaLB); 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR SUPFAM; SSF51045; WW domain; 2.
DR PROSITE; PS50004; C2; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS01159; WW_DOMAIN_1; 2.
DR PROSITE; PS50020; WW_DOMAIN_2; 2.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000005225};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 166..300
FT /note="C2"
FT /evidence="ECO:0000259|PROSITE:PS50004"
FT DOMAIN 807..840
FT /note="WW"
FT /evidence="ECO:0000259|PROSITE:PS50020"
FT DOMAIN 985..1018
FT /note="WW"
FT /evidence="ECO:0000259|PROSITE:PS50020"
FT DOMAIN 1237..1572
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 344..452
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 466..796
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1024..1069
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1161..1187
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 373..410
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 557..626
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 640..671
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 777..796
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1163..1181
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 1540
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 1572 AA; 175191 MW; 4C432D6711558BEA CRC64;
MASSAREHLL FVRRRNPQMR YTLSPENLQS LAAQSSMPEN MALQRANSDT DLVTSESRSS
LTASMYEYTL GQAQNLIIFW DIKEEVDPSD WIGLYHIGES MKWTCKSRNV KGCQQPGGRI
LVRVHPTSYF FPTEIKICFK YYHGISGALR ATTPCITVKN PAVMMGAEGM EGGASGNLHS
RKLVSFTLSD LRAVGLKKGM FFNPDPYLKM SIQPGKKSSF PTCAHHGQER RSTIISNTTN
PIWHREKYSF FALLTDVLEI EIKDKFAKSR PIIKRFLGKL TIPVQRLLER QAIGDQMLSY
NLGRRLPADH VSGYLQFKVE VTSSVHEDAS PEAVGTILGV SAVNGDLGSP SDDEDLPGSH
HDGPVCANGP VSEESTADGT PKHSFRTSST LEIDTEELTS TSSRASPPRG RQDSLNDYLD
AIEHNGHSRP GAGPCSERSM GASPKLRSSF PTDTRLNAML HIDSDEEDHE FQQDLGYPSS
LEEEGGLIMV SRASRADDDA SLTSQTKPED NAPENEDASA QEASSEDKPD NLPELADSSL
PSGPAPEEGE GGQEPQPGVD QGSTELCGSQ EVDQPTSGAD TGTSDTSGGS RRVVSETESL
DQGSEPSQVS SETEPSDPAR TESVSEASTR PEGESDLEGA DSSCNESVTT QLSSVETRCS
SLESARFPET PAFSSQEEED GACAAEPAGS GPAEGAQDSA RTAGSLPAVQ VPGGEEEGPG
PEPAAVPDQE ELGEVWQRRG SLEGAAAAAP ESGDQEEGDA GGAQGACEGA TAQEEGAAGG
SQANGHQPLR SLPSVRQDVS RYQRVDEALP PNWEARIDSH GRIFYVDHVN RTTTWQRPTA
PPAPQVLQRS NSIQQMEQLN RRYQSIRRTM TNERPEENSN VLDGAGEDAD FHQASADFRR
ENVLPHSTSR SRLTLLLQSP PVKFLISPEF FTVLHSNPSA YRMFTNNTCL KHMITKVRRD
THHFERYQHN RDLVGFLNMF ANKQLELPRG WEMKHDHQGK AFFVDHNSRT TTFIDPRLPL
QSSRPTSALV HRQHLTRQRS HSAGEVGEDS RHAGPPVLPR PSSTFNTVSR PQYQDMVPVA
YNDKIVAFLR QPNIFEILQE RQPDLTRNHS LREKIQFIRT EGTPGLVRLS SDADLVMLLS
LFEEEIMSYV PPHALLHPSY CQSPRGSPVS SPQNSPGTQR ANARAPAPYK RDFEAKLRNF
YRKLETKGYG QGPGKLKLII RRDHLLEDAF NQIMGYSRKD LQRNKLYVTF VGEEGLDYSG
PSREFFFLVS RELFNPYYGL FEYSANDTYT VQISPMSAFV DNHHEWFRFS GRILGLALIH
QYLLDAFFTR PFYKALLRIL CDLSDLEYLD EEFHQSLQWM KDNDIHDILD LTFTVNEEVF
GQITERELKP GGANIPVTEK NKKEYIERMV KWRIERGVVQ QTESLVRGFY EVVDARLVSV
FDARELELVI AGTAEIDLSD WRNNTEYRGG YHDNHIVIRW FWAAVERFNN EQRLRLLQFV
TGTSSIPYEG FASLRGSNGP RRFCVEKWGK ITALPRAHTC FNRLDLPPYP SFSMLYEKLL
TAVEETSTFG LE
//
