ID H1G4K1_9GAMM Unreviewed; 728 AA.
AC H1G4K1;
DT 21-MAR-2012, integrated into UniProtKB/TrEMBL.
DT 21-MAR-2012, sequence version 1.
DT 24-JAN-2024, entry version 49.
DE SubName: Full=Radical SAM protein {ECO:0000313|EMBL:EHQ52738.1};
GN ORFNames=ECTPHS_08608 {ECO:0000313|EMBL:EHQ52738.1};
OS Ectothiorhodospira sp. PHS-1.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Chromatiales;
OC Ectothiorhodospiraceae; Ectothiorhodospira.
OX NCBI_TaxID=519989 {ECO:0000313|EMBL:EHQ52738.1, ECO:0000313|Proteomes:UP000005314};
RN [1] {ECO:0000313|EMBL:EHQ52738.1, ECO:0000313|Proteomes:UP000005314}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PHS-1 {ECO:0000313|EMBL:EHQ52738.1,
RC ECO:0000313|Proteomes:UP000005314};
RA Saltikov C.W., Zargar K., Conrad A., Bernick D., Lowe T.M., Stolc V.,
RA Hoeft S., Oremland R.S., Stolz J.;
RT "ArxA, a new clade of arsenite oxidase within the DMSO family of molybdenum
RT oxidoreductases.";
RL Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01251};
CC Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC cysteines and an exchangeable S-adenosyl-L-methionine.
CC {ECO:0000256|HAMAP-Rule:MF_01251};
CC -!- SIMILARITY: Belongs to the UPF0313 family. {ECO:0000256|HAMAP-
CC Rule:MF_01251}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EHQ52738.1}.
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DR EMBL; AGBG01000016; EHQ52738.1; -; Genomic_DNA.
DR RefSeq; WP_008932305.1; NZ_AGBG01000016.1.
DR AlphaFoldDB; H1G4K1; -.
DR STRING; 519989.ECTPHS_08608; -.
DR PATRIC; fig|519989.5.peg.1704; -.
DR eggNOG; COG1032; Bacteria.
DR OrthoDB; 9803479at2; -.
DR Proteomes; UP000005314; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR HAMAP; MF_01251; UPF0313; 1.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR020612; Methylthiotransferase_CS.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR InterPro; IPR022946; UPF0313.
DR InterPro; IPR024560; UPF0313_C.
DR InterPro; IPR013704; UPF0313_N.
DR NCBIfam; TIGR03904; SAM_YgiQ; 1.
DR PANTHER; PTHR32331; UPF0313 PROTEIN YGIQ; 1.
DR PANTHER; PTHR32331:SF0; UPF0313 PROTEIN YGIQ; 1.
DR Pfam; PF11842; DUF3362; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR Pfam; PF08497; Radical_SAM_N; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SFLD; SFLDG01069; UPF0313; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS01278; MTTASE_RADICAL; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485, ECO:0000256|HAMAP-Rule:MF_01251};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|HAMAP-Rule:MF_01251};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|HAMAP-
KW Rule:MF_01251};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|HAMAP-
KW Rule:MF_01251};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691, ECO:0000256|HAMAP-
KW Rule:MF_01251}.
FT DOMAIN 374..639
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
FT REGION 674..728
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 695..713
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 388
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01251"
FT BINDING 392
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01251"
FT BINDING 395
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01251"
SQ SEQUENCE 728 AA; 82360 MW; 48FD4544E5A4A702 CRC64;
MTPSAVFRPL FSYRSYWAAR FGVAPFLPMS RHEMDQLGWD SCDVILVTGD AYIDHPSFGV
AIIGRLLEAQ GFRVGVISQP DWRSSEPFQA LGRPNLFFGV TAGNMDSMVN RYTADRRLRS
NDAYSPDDEG GRRPDRAVIV YSQRVREAYR DVPVIIGGIE ASLRRVAHYD YWSDKVRRSV
LLDSRADLLI YGNGERALVE AAHRIARGEH PRDMKDLRGT AFLLDTPPGD WHVLDSTLVD
RPGPVQRHRD PYCEEIPEQC GPGQAVAWGK AAGAQVIRVQ RRALTAHPRE RTVIRLPDCD
TVQDDPVLYA HASRLIHLET NPGNARALVQ GHGDKDLWIN PPPIPLSTIE LDQLFDLPFA
RAPHPAYRGA RIPAWEMIRF SVNIMRGCFG GCTFCSITEH EGRIIQSRSE DSIEREVDEI
RDKVEGFTGI ISDLGGPTAN MYQLGCKDKA IEAHCRRLSC VHPRICRNLN TDHGPLIRLY
RRIRQRPGVK KVLIASGLRY DLALESKEYV RELVTHHVGG YLKIAPEHTQ PGPLSRMMKP
GIEVYERFRE LFERFSREAG KEQYLVPYFI AAHPGTTDED MLHLALWLKH NGFRLDQVQA
FLPGPMALAT AMYHAERDPL KPVTRGSERV STAKGLRQRR LHKAFLRYHD PENWPLLREA
LQRMGRADLI GNGKRHLVPR FQPAGTGTTG EGRRRPVQAA ERPASRRKSP RGPAPDRGAV
AGRTRKRR
//
