ID H1HM69_9BACT Unreviewed; 543 AA.
AC H1HM69;
DT 21-MAR-2012, integrated into UniProtKB/TrEMBL.
DT 21-MAR-2012, sequence version 1.
DT 27-MAR-2024, entry version 47.
DE RecName: Full=Extracellular endo-alpha-(1->5)-L-arabinanase C-terminal domain-containing protein {ECO:0000259|Pfam:PF16369};
GN ORFNames=HMPREF9944_01263 {ECO:0000313|EMBL:EHO70789.1};
OS Segatella maculosa OT 289.
OC Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Prevotellaceae;
OC Segatella.
OX NCBI_TaxID=999422 {ECO:0000313|EMBL:EHO70789.1, ECO:0000313|Proteomes:UP000003167};
RN [1] {ECO:0000313|EMBL:EHO70789.1, ECO:0000313|Proteomes:UP000003167}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=OT 289 {ECO:0000313|EMBL:EHO70789.1,
RC ECO:0000313|Proteomes:UP000003167};
RG The Broad Institute Genome Sequencing Platform;
RA Earl A., Ward D., Feldgarden M., Gevers D., Izard J., Blanton J.M.,
RA Mathney J., Tanner A.C., Dewhirst F.E., Young S.K., Zeng Q., Gargeya S.,
RA Fitzgerald M., Haas B., Abouelleil A., Alvarado L., Arachchi H.M.,
RA Berlin A., Chapman S.B., Gearin G., Goldberg J., Griggs A., Gujja S.,
RA Hansen M., Heiman D., Howarth C., Larimer J., Lui A., MacDonald P.J.P.,
RA McCowen C., Montmayeur A., Murphy C., Neiman D., Pearson M., Priest M.,
RA Roberts A., Saif S., Shea T., Sisk P., Stolte C., Sykes S., Wortman J.,
RA Nusbaum C., Birren B.;
RT "The Genome Sequence of Prevotella maculosa OT 289.";
RL Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- PATHWAY: Glycan metabolism; L-arabinan degradation.
CC {ECO:0000256|ARBA:ARBA00004834}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EHO70789.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AGEK01000024; EHO70789.1; -; Genomic_DNA.
DR AlphaFoldDB; H1HM69; -.
DR STRING; 999422.HMPREF9944_01263; -.
DR PATRIC; fig|999422.3.peg.1304; -.
DR HOGENOM; CLU_009397_1_3_10; -.
DR OrthoDB; 9801455at2; -.
DR Proteomes; UP000003167; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR CDD; cd08998; GH43_Arb43a-like; 1.
DR Gene3D; 2.40.128.10; -; 1.
DR InterPro; IPR032291; Abn2_C.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR43301; ARABINAN ENDO-1,5-ALPHA-L-ARABINOSIDASE; 1.
DR PANTHER; PTHR43301:SF3; ARABINOSIDASE-RELATED; 1.
DR Pfam; PF16369; GH43_C; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|RuleBase:RU361187};
KW Hydrolase {ECO:0000256|RuleBase:RU361187};
KW Reference proteome {ECO:0000313|Proteomes:UP000003167};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..18
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 19..543
FT /note="Extracellular endo-alpha-(1->5)-L-arabinanase C-
FT terminal domain-containing protein"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003550515"
FT DOMAIN 436..540
FT /note="Extracellular endo-alpha-(1->5)-L-arabinanase C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF16369"
FT ACT_SITE 70
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 310
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 243
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 543 AA; 61971 MW; 60DA9CF344F09BEA CRC64;
MKTNSMVWIS FLAAIAFAGC KEDDNIEYEN PGSKVQTDVT VTDGQNMADD YTPIADWGNR
FRWNLANVHD PSVVRADDGY YYMYQTDASY GNAHAGEGQS RGHFYCRRSK DLINWEFMGP
TLHGVPTWVK SKLNEIRKDM GLAPSSIDFH NQTQFGFWAP CVRRIRRDLY RMYYVVTLPG
TINGTGTWSE RCFIGLMETS NPADIDTWTD KGYVITNYSD RGLDFKVNAT DYARCYFKYN
AIDPSLLINE KGEHWLVYGS WHSGFAALQL DPVTGKPLHA LGNPWGAENE TAYGKLIYTR
KFGDRWQASE APEVVWHEGY YYLFMAYDEL SVAYNTRVVR SRNIEGPYYD ITGRDVTNRG
GDAYPILTHP YRFGSDHGWV GISHCAVFND DKGNWYYASQ QRFPENYKGN AGSNALMVGG
VRAIVWTSDG WPLVLPERYA GIAQKTISES ELLGDWQHIN LVYNYKRQDA SVSMTLAANH
TVKSGWKTGK AWRFDASKNV LTIDNEQFYL RRELDWEANP RKATIVYVGL SSNGKTTYWG
KKV
//
