ID H1PTN8_9FUSO Unreviewed; 569 AA.
AC H1PTN8;
DT 21-MAR-2012, integrated into UniProtKB/TrEMBL.
DT 21-MAR-2012, sequence version 1.
DT 27-MAR-2024, entry version 48.
DE RecName: Full=Urocanate reductase {ECO:0000256|RuleBase:RU366062};
DE EC=1.3.99.33 {ECO:0000256|RuleBase:RU366062};
GN ORFNames=HMPREF0402_01781 {ECO:0000313|EMBL:EHO80708.1};
OS Fusobacterium ulcerans 12-1B.
OC Bacteria; Fusobacteriota; Fusobacteriia; Fusobacteriales; Fusobacteriaceae;
OC Fusobacterium.
OX NCBI_TaxID=457404 {ECO:0000313|EMBL:EHO80708.1, ECO:0000313|Proteomes:UP000003233};
RN [1] {ECO:0000313|EMBL:EHO80708.1, ECO:0000313|Proteomes:UP000003233}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=12_1B {ECO:0000313|EMBL:EHO80708.1,
RC ECO:0000313|Proteomes:UP000003233};
RG The Broad Institute Genome Sequencing Platform;
RA Earl A., Ward D., Feldgarden M., Gevers D., Strauss J., Ambrose C.E.,
RA Allen-Vercoe E., Walker B., Young S.K., Zeng Q., Gargeya S., Fitzgerald M.,
RA Haas B., Abouelleil A., Alvarado L., Arachchi H.M., Berlin A.M.,
RA Chapman S.B., Goldberg J., Griggs A., Gujja S., Hansen M., Howarth C.,
RA Imamovic A., Larimer J., McCowen C., Montmayeur A., Murphy C., Neiman D.,
RA Pearson M., Priest M., Roberts A., Saif S., Shea T., Sisk P., Sykes S.,
RA Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Fusobacterium ulcerans 12_1B.";
RL Submitted (JUL-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=A + dihydrourocanate = AH2 + urocanate; Xref=Rhea:RHEA:36059,
CC ChEBI:CHEBI:13193, ChEBI:CHEBI:17499, ChEBI:CHEBI:27247,
CC ChEBI:CHEBI:72991; EC=1.3.99.33;
CC Evidence={ECO:0000256|RuleBase:RU366062};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|RuleBase:RU366062};
CC Note=Binds 1 FAD per subunit. {ECO:0000256|RuleBase:RU366062};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|RuleBase:RU366062};
CC Note=Binds 1 FMN covalently per subunit.
CC {ECO:0000256|RuleBase:RU366062};
CC -!- SIMILARITY: Belongs to the FAD-dependent oxidoreductase 2 family.
CC FRD/SDH subfamily. {ECO:0000256|RuleBase:RU366062}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EHO80708.1}.
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DR EMBL; AGWJ02000021; EHO80708.1; -; Genomic_DNA.
DR RefSeq; WP_008697321.1; NZ_KE161008.1.
DR AlphaFoldDB; H1PTN8; -.
DR PATRIC; fig|457404.5.peg.2321; -.
DR HOGENOM; CLU_011398_4_0_0; -.
DR BioCyc; FSP457404-HMP:GTSQ-1791-MONOMER; -.
DR Proteomes; UP000003233; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:InterPro.
DR GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR Gene3D; 3.90.1010.20; -; 1.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR Gene3D; 3.90.700.10; Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain; 1.
DR InterPro; IPR003953; FAD-binding_2.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR010960; Flavocytochrome_c.
DR InterPro; IPR007329; FMN-bd.
DR InterPro; IPR027477; Succ_DH/fumarate_Rdtase_cat_sf.
DR NCBIfam; TIGR01813; flavo_cyto_c; 1.
DR PANTHER; PTHR43400:SF7; FAD_BINDING_2 DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR43400; FUMARATE REDUCTASE; 1.
DR Pfam; PF00890; FAD_binding_2; 1.
DR Pfam; PF04205; FMN_bind; 1.
DR PRINTS; PR00368; FADPNR.
DR PRINTS; PR00411; PNDRDTASEI.
DR SMART; SM00900; FMN_bind; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR SUPFAM; SSF56425; Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|RuleBase:RU366062};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630,
KW ECO:0000256|RuleBase:RU366062};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000256|RuleBase:RU366062}; Signal {ECO:0000256|RuleBase:RU366062}.
FT SIGNAL 1..22
FT /evidence="ECO:0000256|RuleBase:RU366062"
FT CHAIN 23..569
FT /note="Urocanate reductase"
FT /evidence="ECO:0000256|RuleBase:RU366062"
FT /id="PRO_5022261665"
FT DOMAIN 31..104
FT /note="FMN-binding"
FT /evidence="ECO:0000259|SMART:SM00900"
SQ SEQUENCE 569 AA; 59707 MW; DD5E6ADDCED71AFB CRC64;
MRKKLLCLLS ALTIMISSVA SAKTIEGVGA GYKGDIKVNV TYEGNEIKGV EILKHEETAF
TKKAMEQVTK EIVENQSVEV DNVAGATYTS EGIKEAVGAA VAQAGIKLVS KAPAKKADAV
LTDISTDIVV VGGGGAGLTA AIAAKEKGAN VILLEKMAML GGNTNYATGG INAANTSLQK
KLGIEDSEEL FYNDTMKGGK NKNNPELLKK MTDESKHIID WLISKGTDLT EVSFSGGQSV
KRIHRPTGGK AVGPVVVAAL SDTADKLGIE IRTESEVTKL IKTGDKVTGV EVKHKGQTYV
ISAKAVVMAT GGFGANPAMI EEYNPALKGF GSTNSPAITG EGIKMVKAAG ADLVDMPEIQ
THPTVVHNNT AMITESVRGE GAILINRDGK RFINELETRD VVSKAELAQK GASAFLVFDQ
GTRENLSAIN GYVKQGFAVE ASTLEELAGK VGIEPKTFVE TITTYNGYVK AGEDKDFGKK
GLPRELVKAP FYAIEVSPAI HHTMGGVRIN TDTQVLTPEG KVIPGLYAAG EITGGVHGGN
RIGGNAVTDI TVFGKIAGEN AADFVKNSK
//
