ID H2KQU2_CLOSI Unreviewed; 1009 AA.
AC H2KQU2;
DT 21-MAR-2012, integrated into UniProtKB/TrEMBL.
DT 21-MAR-2012, sequence version 1.
DT 27-MAR-2024, entry version 50.
DE RecName: Full=Coatomer subunit beta' {ECO:0000256|PIRNR:PIRNR005567};
GN ORFNames=CLF_104710 {ECO:0000313|EMBL:GAA38251.2}, CSKR_102765
GN {ECO:0000313|EMBL:KAG5441137.1};
OS Clonorchis sinensis (Chinese liver fluke).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Platyhelminthes; Trematoda;
OC Digenea; Opisthorchiida; Opisthorchiata; Opisthorchiidae; Clonorchis.
OX NCBI_TaxID=79923 {ECO:0000313|EMBL:GAA38251.2, ECO:0000313|Proteomes:UP000008909};
RN [1] {ECO:0000313|EMBL:GAA38251.2}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Henan {ECO:0000313|EMBL:GAA38251.2};
RX PubMed=22023798; DOI=10.1186/gb-2011-12-10-r107;
RA Wang X., Chen W., Huang Y., Sun J., Men J., Liu H., Luo F., Guo L., Lv X.,
RA Deng C., Zhou C., Fan Y., Li X., Huang L., Hu Y., Liang C., Hu X., Xu J.,
RA Yu X.;
RT "The draft genome of the carcinogenic human liver fluke Clonorchis
RT sinensis.";
RL Genome Biol. 12:R107-R107(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Henan;
RA Wang X., Huang Y., Chen W., Liu H., Guo L., Chen Y., Luo F., Zhou W.,
RA Sun J., Mao Q., Liang P., Zhou C., Tian Y., Men J., Lv X., Huang L.,
RA Zhou J., Hu Y., Li R., Zhang F., Lei H., Li X., Hu X., Liang C., Xu J.,
RA Wu Z., Yu X.;
RT "The genome and transcriptome sequence of Clonorchis sinensis provide
RT insights into the carcinogenic liver fluke.";
RL Submitted (OCT-2011) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:KAG5441137.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Cs-k2 {ECO:0000313|EMBL:KAG5441137.1};
RX PubMed=29454982;
RA Wang D., Korhonen P.K., Gasser R.B., Young N.D.;
RT "Improved genomic resources and new bioinformatic workflow for the
RT carcinogenic parasite Clonorchis sinensis: Biotechnological implications.";
RL Biotechnol. Adv. 36:894-904(2018).
RN [4] {ECO:0000313|EMBL:KAG5441137.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Cs-k2 {ECO:0000313|EMBL:KAG5441137.1};
RX PubMed=33677057;
RA Young N.D., Stroehlein A.J., Kinkar L., Wang T., Sohn W.M., Chang B.C.H.,
RA Kaur P., Weisz D., Dudchenko O., Aiden E.L., Korhonen P.K., Gasser R.B.;
RT "High-quality reference genome for Clonorchis sinensis.";
RL Genomics 0:0-0(2021).
CC -!- FUNCTION: The coatomer is a cytosolic protein complex that binds to
CC dilysine motifs and reversibly associates with Golgi non-clathrin-
CC coated vesicles, which further mediate biosynthetic protein transport
CC from the ER, via the Golgi up to the trans Golgi network. Coatomer
CC complex is required for budding from Golgi membranes, and is essential
CC for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.
CC {ECO:0000256|PIRNR:PIRNR005567}.
CC -!- SUBUNIT: Oligomeric complex that consists of at least the alpha, beta,
CC beta', gamma, delta, epsilon and zeta subunits.
CC {ECO:0000256|PIRNR:PIRNR005567}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, COPI-coated vesicle membrane
CC {ECO:0000256|ARBA:ARBA00004347, ECO:0000256|PIRNR:PIRNR005567};
CC Peripheral membrane protein {ECO:0000256|ARBA:ARBA00004347,
CC ECO:0000256|PIRNR:PIRNR005567}; Cytoplasmic side
CC {ECO:0000256|ARBA:ARBA00004347, ECO:0000256|PIRNR:PIRNR005567}. Golgi
CC apparatus membrane {ECO:0000256|PIRNR:PIRNR005567}; Peripheral membrane
CC protein {ECO:0000256|PIRNR:PIRNR005567}; Cytoplasmic side
CC {ECO:0000256|PIRNR:PIRNR005567}. Membrane
CC {ECO:0000256|ARBA:ARBA00004287}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004287}; Cytoplasmic side
CC {ECO:0000256|ARBA:ARBA00004287}. Note=The coatomer is cytoplasmic or
CC polymerized on the cytoplasmic side of the Golgi, as well as on the
CC vesicles/buds originating from it. {ECO:0000256|PIRNR:PIRNR005567}.
CC -!- SIMILARITY: Belongs to the WD repeat COPB2 family.
CC {ECO:0000256|ARBA:ARBA00010844, ECO:0000256|PIRNR:PIRNR005567}.
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DR EMBL; DF143056; GAA38251.2; -; Genomic_DNA.
DR EMBL; NIRI02000077; KAG5441137.1; -; Genomic_DNA.
DR AlphaFoldDB; H2KQU2; -.
DR STRING; 79923.H2KQU2; -.
DR InParanoid; H2KQU2; -.
DR Proteomes; UP000008909; Unassembled WGS sequence.
DR Proteomes; UP000286415; Chromosome 7.
DR GO; GO:0030663; C:COPI-coated vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030117; C:membrane coat; IEA:InterPro.
DR GO; GO:0005198; F:structural molecule activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006886; P:intracellular protein transport; IEA:UniProtKB-UniRule.
DR GO; GO:0016192; P:vesicle-mediated transport; IEA:UniProtKB-KW.
DR CDD; cd22947; Coatomer_WDAD_beta-like; 1.
DR CDD; cd00200; WD40; 1.
DR Gene3D; 1.25.40.470; -; 1.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR006692; Coatomer_WD-assoc_reg.
DR InterPro; IPR016453; COPB2.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR011044; Quino_amine_DH_bsu.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR001680; WD40_rpt.
DR PANTHER; PTHR19876; COATOMER; 1.
DR PANTHER; PTHR19876:SF2; COATOMER SUBUNIT BETA; 1.
DR Pfam; PF04053; Coatomer_WDAD; 1.
DR Pfam; PF00400; WD40; 6.
DR PIRSF; PIRSF005567; Coatomer_beta'_subunit; 2.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF50978; WD40 repeat-like; 1.
DR SUPFAM; SSF50969; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 5.
DR PROSITE; PS50294; WD_REPEATS_REGION; 4.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|PIRNR:PIRNR005567};
KW Cytoplasmic vesicle {ECO:0000256|PIRNR:PIRNR005567};
KW ER-Golgi transport {ECO:0000256|ARBA:ARBA00022892,
KW ECO:0000256|PIRNR:PIRNR005567};
KW Golgi apparatus {ECO:0000256|ARBA:ARBA00023034,
KW ECO:0000256|PIRNR:PIRNR005567};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|PIRNR:PIRNR005567};
KW Protein transport {ECO:0000256|ARBA:ARBA00022927,
KW ECO:0000256|PIRNR:PIRNR005567};
KW Reference proteome {ECO:0000313|Proteomes:UP000008909};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|PIRNR:PIRNR005567};
KW WD repeat {ECO:0000256|ARBA:ARBA00022574, ECO:0000256|PROSITE-
KW ProRule:PRU00221}.
FT REPEAT 11..52
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REPEAT 95..127
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REPEAT 138..180
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REPEAT 181..224
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REPEAT 225..266
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT DOMAIN 331..774
FT /note="Coatomer WD associated region"
FT /evidence="ECO:0000259|Pfam:PF04053"
FT REGION 899..1009
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 913..971
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1009 AA; 112979 MW; AC6C0F38FD66BDE5 CRC64;
MPLRLDIKRK LLARSDRVKA VDLHPTEPWI CAALYNGNVH VWNIEGQQLI KTLEVCTLPV
RAVKFVARKN WIVTGSDDLQ LRVFNYNTLE RVQQIEAHSD YIRSIAVHPT QPFLLTASDD
MLIRLWDWEK NWTCAQVFEG HSHYVMQLVF NPKDNNTFAS ASLDHTVKVW SLGSSSPNFT
LEGHERGVNC VDYYIFGDKP YLASGADDKT VRIWDYQTKA CVQTLEGHAQ NISAVVFHPE
LPIILTGSED GTVRVWHSGT YRLESTLNYG LERIWAMACY RGKQTVAIGY DEGTIVISLG
RDEPAMSMDA SGKVVCARHT ELMQANLRSL TLGPGGVDDI QDGERLPVTF KDMGTSEIYP
QTIEHNANGR FVAVCGDGEY IIYTAMALRN KTFGQAQEFV WSQADASMYA VRESNAIVKV
YKQFKEVRTF KLDYGAEQIF GGHLLGVRSL TGLTFYDWNT GRLVRRIDIS PRSVYWNEGG
QLVSLCTNET AFILRYAADA IPEAEPAPGS IEDTDGFEQA FQVVPNGEVN VAVHTGFWYG
DAFLFTTTAN RLCYYVGGEL VTLAHLDRPM HLLGYLAKEN RVYLSDRDLQ IASYSLLLSV
LEYETAVMRG DFSAADTIFP SIPKEQRTKV AQFLEKQGFR SQAMRVTTDV DHKFDLALQL
GDLELCRDLA ADGDPEVNES KWKQLAEAAC RSCRFDLAEE CLARIKDYAS LLLLASSSGN
GQMVKWIGDQ ASTESKDNVA FLARFLLSDL EGCLELLVKA DRLPEAAFFA RTYLPSHVPE
IVELWREWLK KTTKSSSKVA QALANPQEYP NLFPGMENAL AAEKRMKMER AARSRLPAST
YPSQPAMWER DVLSELHTKP EPLFESVPSH TLLNGGTLGS ESEANVARAS PTIEVMIASQ
QNNAKPTLEE DNTPEEKDNE EEEEADEEEE EVDDEGDGED EDDDEDEEDE EEEEENGDAE
EPEDDTLDAE LEQEIATLQL NKMKSKPTTA VAAPPSADAD WGDAGSDFE
//
