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Database: UniProt
Entry: H3FZ98_PRIPA
LinkDB: H3FZ98_PRIPA
Original site: H3FZ98_PRIPA 
ID   H3FZ98_PRIPA            Unreviewed;       981 AA.
AC   H3FZ98;
DT   18-APR-2012, integrated into UniProtKB/TrEMBL.
DT   18-APR-2012, sequence version 1.
DT   07-JUN-2017, entry version 28.
DE   RecName: Full=Non-lysosomal glucosylceramidase {ECO:0000256|PIRNR:PIRNR028944};
DE            Short=NLGase {ECO:0000256|PIRNR:PIRNR028944};
DE            EC=3.2.1.45 {ECO:0000256|PIRNR:PIRNR028944};
GN   Name=WBGene00117201 {ECO:0000313|EnsemblMetazoa:PPA27647};
OS   Pristionchus pacificus (Parasitic nematode).
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Diplogasterida;
OC   Neodiplogasteridae; Pristionchus.
OX   NCBI_TaxID=54126 {ECO:0000313|EnsemblMetazoa:PPA27647};
RN   [1] {ECO:0000313|EnsemblMetazoa:PPA27647}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PS312 {ECO:0000313|EnsemblMetazoa:PPA27647};
RX   PubMed=18806794; DOI=10.1038/ng.227;
RA   Dieterich C., Clifton S.W., Schuster L.N., Chinwalla A.,
RA   Delehaunty K., Dinkelacker I., Fulton L., Fulton R., Godfrey J.,
RA   Minx P., Mitreva M., Roeseler W., Tian H., Witte H., Yang S.P.,
RA   Wilson R.K., Sommer R.J.;
RT   "The Pristionchus pacificus genome provides a unique perspective on
RT   nematode lifestyle and parasitism.";
RL   Nat. Genet. 40:1193-1198(2008).
RN   [2] {ECO:0000313|EnsemblMetazoa:PPA27647}
RP   IDENTIFICATION.
RC   STRAIN=PS312 {ECO:0000313|EnsemblMetazoa:PPA27647};
RG   EnsemblMetazoa;
RL   Submitted (JUN-2015) to UniProtKB.
CC   -!- FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the
CC       conversion of glucosylceramide to free glucose and ceramide.
CC       {ECO:0000256|PIRNR:PIRNR028944}.
CC   -!- CATALYTIC ACTIVITY: D-glucosyl-N-acylsphingosine + H(2)O = D-
CC       glucose + N-acylsphingosine. {ECO:0000256|PIRNR:PIRNR028944}.
CC   -!- SIMILARITY: Belongs to the non-lysosomal glucosylceramidase
CC       family. {ECO:0000256|PIRNR:PIRNR028944}.
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DR   STRING; 54126.PPA27647; -.
DR   EnsemblMetazoa; PPA27647; PPA27647; PPA27647.
DR   eggNOG; KOG2119; Eukaryota.
DR   eggNOG; COG4354; LUCA.
DR   InParanoid; H3FZ98; -.
DR   OMA; HDYQDSS; -.
DR   OrthoDB; EOG091G01JK; -.
DR   Proteomes; UP000005239; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006680; P:glucosylceramide catabolic process; IEA:InterPro.
DR   InterPro; IPR008928; 6-hairpin_glycosidase-like.
DR   InterPro; IPR014551; B_Glucosidase_GBA2-typ.
DR   InterPro; IPR006775; GH116_catalytic.
DR   InterPro; IPR024462; GH116_N.
DR   Pfam; PF04685; DUF608; 1.
DR   Pfam; PF12215; Glyco_hydr_116N; 1.
DR   PIRSF; PIRSF028944; Beta_gluc_GBA2; 1.
DR   SUPFAM; SSF48208; SSF48208; 3.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000005239};
KW   Glycosidase {ECO:0000256|PIRNR:PIRNR028944};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR028944};
KW   Lipid metabolism {ECO:0000256|PIRNR:PIRNR028944};
KW   Membrane {ECO:0000256|PIRNR:PIRNR028944};
KW   Reference proteome {ECO:0000313|Proteomes:UP000005239}.
FT   DOMAIN      105    420       Glyco_hydr_116N. {ECO:0000259|Pfam:
FT                                PF12215}.
FT   DOMAIN      485    859       DUF608. {ECO:0000259|Pfam:PF04685}.
SQ   SEQUENCE   981 AA;  111059 MW;  73029A05ACD5242F CRC64;
     MCQPGAKAGK VKKSRSSRRT VENAEEEADR SGAPPACMSG AGWHARGDKL PEEKRVPFNR
     PSARQVYDAL PFVRRYFMYW VRHTGKKEKL FINTFQPLQH KPYYGVPCGG IGSGSIGRDF
     RGGFCKFGLR PGLIEHKVNV VPSDQFILSV RRPGDADACY QKVLCAADID RSAGQLSAWD
     FDFPETDIAF RGLYPRSWTC YYIAELSLVV VLRQISPVIP HNYKDSSLPA TVFVVDVENR
     SAEDYEVSIA FTFRNGTGYY RWEADPGCSS KEFQYNRDEQ GPGVHGITLE HNIDRMRCTY
     ALGLKTSENP LYHASICRSF QPSANGQGVW KHLRETGDLP DHADEASVTE QAVGICVRTT
     VAAGERRESA AEMALVWHMP VVKFGNGERT YKRRYTRYFD EREEGARALC TYALRERQRW
     EEEIEQWQRP VLEHEHLPLW YKSALFNELY FITDGGSIWF EWDEEWTKRE PHLSEYARNV
     MREHGRFGYL ESWEYRMVNT YDVHFYASFA LAHLWPQLEI SVQAEFIPNR FSIPADQVEH
     VDEVVVRFHM EGDRAPQKTR TRVPHDLGNP AADPWLLTNA YVMHDTGKWK DLNLKFVLTS
     WRDYVCIVKR ERAFLEHVYP AVKSLISQGL TTWDTDGDGM IENFGKADQT YDAWQMEGVS
     AYCGSLWLAA LRVAVELARE IGDDEKANEW NETLQRAKKV FVEKLWTGEY FRFCERSRSR
     DSVMADQLCG IWFLQSVSPE MAEELLPLGH VQSALTKIFE LNVQRFAGGR MGAVNGMRPD
     GKVDRAYIQA DEMWTGVTYA VAAFMIQQGD YQRAFDTAWG TYDACFNRFG LQYQTPEALY
     EKKFYRAIGY MRPLAVWAMQ WALEKHAGLG GANEKRGISE LRETTFATCS KTSVAAKSET
     TVESSTVVTA QPSPAATRSD DDGGSEGYGS DEKESSATVG HVLPPAMKST TTLTTLGGAA
     SDDAASSSGV SSGRDVVVEG L
//
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