ID H3RGC3_PANSE Unreviewed; 183 AA.
AC H3RGC3;
DT 18-APR-2012, integrated into UniProtKB/TrEMBL.
DT 18-APR-2012, sequence version 1.
DT 24-JAN-2024, entry version 61.
DE RecName: Full=Glutathione-regulated potassium-efflux system ancillary protein KefG {ECO:0000256|HAMAP-Rule:MF_01415};
DE AltName: Full=Putative quinone oxidoreductase KefG {ECO:0000256|HAMAP-Rule:MF_01415};
DE EC=1.6.5.2 {ECO:0000256|HAMAP-Rule:MF_01415};
GN Name=kefG {ECO:0000256|HAMAP-Rule:MF_01415,
GN ECO:0000313|EMBL:EHT99480.1};
GN ORFNames=CKS_1606 {ECO:0000313|EMBL:EHT99480.1}, DSJ_00605
GN {ECO:0000313|EMBL:ARF48028.1};
OS Pantoea stewartii subsp. stewartii DC283.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Erwiniaceae; Pantoea.
OX NCBI_TaxID=660596 {ECO:0000313|EMBL:EHT99480.1, ECO:0000313|Proteomes:UP000005050};
RN [1] {ECO:0000313|EMBL:EHT99480.1, ECO:0000313|Proteomes:UP000005050}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DC283 {ECO:0000313|EMBL:EHT99480.1,
RC ECO:0000313|Proteomes:UP000005050};
RX PubMed=22111898; DOI=10.1111/j.1365-2958.2011.07926.x;
RA Wang X., Yang F., von Bodman S.B.;
RT "The genetic and structural basis of two distinct terminal side branch
RT residues in stewartan and amylovoran exopolysaccharides and their potential
RT role in host adaptation.";
RL Mol. Microbiol. 83:195-207(2012).
RN [2] {ECO:0000313|EMBL:EHT99480.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=DC283 {ECO:0000313|EMBL:EHT99480.1};
RA Biehl B.S., Ding Y., Dugan-Rocha S.P., Gibbs R.A., Glasner J.D., Kovar C.,
RA Muzny D.M., Neeno-Eckwall E.C., Perna N.T., Qin X., von Bodman S.B.,
RA Weinstock G.M.;
RL Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:ARF48028.1, ECO:0000313|Proteomes:UP000192380}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DC283 {ECO:0000313|EMBL:ARF48028.1,
RC ECO:0000313|Proteomes:UP000192380};
RA Duong D.A., Stevens A.M., Jensen R.V.;
RT "Complete Genome Assembly of Pantoea stewartii subsp. stewartii DC283, a
RT Corn Pathogen.";
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Regulatory subunit of a potassium efflux system that confers
CC protection against electrophiles. Required for full activity of KefB.
CC {ECO:0000256|HAMAP-Rule:MF_01415}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a quinone + H(+) + NADH = a quinol + NAD(+);
CC Xref=Rhea:RHEA:46160, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124; EC=1.6.5.2;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01415};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a quinone + H(+) + NADPH = a quinol + NADP(+);
CC Xref=Rhea:RHEA:46164, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:132124; EC=1.6.5.2;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01415};
CC -!- SUBUNIT: Interacts with KefB. {ECO:0000256|HAMAP-Rule:MF_01415}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000256|HAMAP-
CC Rule:MF_01415}; Peripheral membrane protein {ECO:0000256|HAMAP-
CC Rule:MF_01415}; Cytoplasmic side {ECO:0000256|HAMAP-Rule:MF_01415}.
CC -!- SIMILARITY: Belongs to the NAD(P)H dehydrogenase (quinone) family. KefG
CC subfamily. {ECO:0000256|HAMAP-Rule:MF_01415}.
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DR EMBL; CP017581; ARF48028.1; -; Genomic_DNA.
DR EMBL; AHIE01000025; EHT99480.1; -; Genomic_DNA.
DR RefSeq; WP_006120565.1; NZ_CP017581.1.
DR AlphaFoldDB; H3RGC3; -.
DR STRING; 660596.DSJ_00605; -.
DR GeneID; 61253023; -.
DR KEGG; pstw:DSJ_00605; -.
DR PATRIC; fig|660596.6.peg.3233; -.
DR eggNOG; COG2249; Bacteria.
DR OrthoDB; 9798454at2; -.
DR Proteomes; UP000005050; Unassembled WGS sequence.
DR Proteomes; UP000192380; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0003955; F:NAD(P)H dehydrogenase (quinone) activity; IEA:UniProtKB-UniRule.
DR GO; GO:1901381; P:positive regulation of potassium ion transmembrane transport; IEA:UniProtKB-UniRule.
DR GO; GO:0006813; P:potassium ion transport; IEA:InterPro.
DR Gene3D; 3.40.50.360; -; 1.
DR HAMAP; MF_01415; K_H_efflux_KefG; 1.
DR InterPro; IPR003680; Flavodoxin_fold.
DR InterPro; IPR029039; Flavoprotein-like_sf.
DR InterPro; IPR023947; K_H_efflux_KefG.
DR InterPro; IPR046980; KefG/KefF.
DR PANTHER; PTHR47307; GLUTATHIONE-REGULATED POTASSIUM-EFFLUX SYSTEM ANCILLARY PROTEIN KEFG; 1.
DR PANTHER; PTHR47307:SF1; GLUTATHIONE-REGULATED POTASSIUM-EFFLUX SYSTEM ANCILLARY PROTEIN KEFG; 1.
DR Pfam; PF02525; Flavodoxin_2; 1.
DR SUPFAM; SSF52218; Flavoproteins; 1.
PE 3: Inferred from homology;
KW Cell inner membrane {ECO:0000256|HAMAP-Rule:MF_01415};
KW Cell membrane {ECO:0000256|HAMAP-Rule:MF_01415};
KW Membrane {ECO:0000256|HAMAP-Rule:MF_01415};
KW NAD {ECO:0000256|HAMAP-Rule:MF_01415};
KW Oxidoreductase {ECO:0000256|HAMAP-Rule:MF_01415,
KW ECO:0000313|EMBL:EHT99480.1}.
FT DOMAIN 6..173
FT /note="Flavodoxin-like fold"
FT /evidence="ECO:0000259|Pfam:PF02525"
SQ SEQUENCE 183 AA; 21271 MW; F7F292EC98035D77 CRC64;
MSQPPKILLL FAHPEAQDSI ANRILLDAAR PLEHVTVHDL YAAYPDFFID IHHEQQLLRE
HDVIIFQHPL YTYSCPALLK EWLDRVLSRG FANGIDGNAL EGKYWRSIVT TGEPETAFQQ
HGLNRYSMND ILRPFELTAQ MCRMHWMSPM IIYWARRQQP DLLKKYARAY SDWLAAPLPN
GGL
//
