ID H6LH77_ACEWD Unreviewed; 1241 AA.
AC H6LH77;
DT 18-APR-2012, integrated into UniProtKB/TrEMBL.
DT 18-APR-2012, sequence version 1.
DT 27-MAR-2024, entry version 56.
DE SubName: Full=Phosphoribosylformylglycinamidine synthase II {ECO:0000313|EMBL:AFA48415.1};
DE EC=6.3.5.3 {ECO:0000313|EMBL:AFA48415.1};
GN Name=purL {ECO:0000313|EMBL:AFA48415.1};
GN OrderedLocusNames=Awo_c16330 {ECO:0000313|EMBL:AFA48415.1};
OS Acetobacterium woodii (strain ATCC 29683 / DSM 1030 / JCM 2381 / KCTC 1655
OS / WB1).
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Eubacteriaceae;
OC Acetobacterium.
OX NCBI_TaxID=931626 {ECO:0000313|EMBL:AFA48415.1, ECO:0000313|Proteomes:UP000007177};
RN [1] {ECO:0000313|Proteomes:UP000007177}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29683 / DSM 1030 / JCM 2381 / KCTC 1655 / WB1
RC {ECO:0000313|Proteomes:UP000007177};
RA Poehlein A., Schmidt S., Kaster A.-K., Goenrich M., Vollmers J.,
RA Thuermer A., Gottschalk G., Thauer R.K., Daniel R., Mueller V.;
RT "Complete genome sequence of Acetobacterium woodii.";
RL Submitted (JUL-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:AFA48415.1, ECO:0000313|Proteomes:UP000007177}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29683 / DSM 1030 / JCM 2381 / KCTC 1655 / WB1
RC {ECO:0000313|Proteomes:UP000007177};
RX PubMed=22479398; DOI=10.1371/journal.pone.0033439;
RA Poehlein A., Schmidt S., Kaster A.K., Goenrich M., Vollmers J., Thurmer A.,
RA Bertsch J., Schuchmann K., Voigt B., Hecker M., Daniel R., Thauer R.K.,
RA Gottschalk G., Muller V.;
RT "An ancient pathway combining carbon dioxide fixation with the generation
RT and utilization of a sodium ion gradient for ATP synthesis.";
RL PLoS ONE 7:E33439-E33439(2012).
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DR EMBL; CP002987; AFA48415.1; -; Genomic_DNA.
DR RefSeq; WP_014356018.1; NC_016894.1.
DR AlphaFoldDB; H6LH77; -.
DR STRING; 931626.Awo_c16330; -.
DR KEGG; awo:Awo_c16330; -.
DR eggNOG; COG0046; Bacteria.
DR eggNOG; COG0047; Bacteria.
DR HOGENOM; CLU_003100_2_0_9; -.
DR OrthoDB; 9804441at2; -.
DR Proteomes; UP000007177; Chromosome.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004642; F:phosphoribosylformylglycinamidine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006164; P:purine nucleotide biosynthetic process; IEA:UniProtKB-KW.
DR CDD; cd02203; PurL_repeat1; 1.
DR CDD; cd02204; PurL_repeat2; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.90.650.10; PurM-like C-terminal domain; 2.
DR Gene3D; 3.30.1330.10; PurM-like, N-terminal domain; 2.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR010141; FGAM_synthase.
DR InterPro; IPR041609; PurL_linker.
DR InterPro; IPR010918; PurM-like_C_dom.
DR InterPro; IPR036676; PurM-like_C_sf.
DR InterPro; IPR036921; PurM-like_N_sf.
DR NCBIfam; TIGR01857; FGAM-synthase; 1.
DR PANTHER; PTHR10099; PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE; 1.
DR PANTHER; PTHR10099:SF1; PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE; 1.
DR Pfam; PF02769; AIRS_C; 1.
DR Pfam; PF18072; FGAR-AT_linker; 1.
DR Pfam; PF13507; GATase_5; 1.
DR SMART; SM01211; GATase_5; 1.
DR SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
DR SUPFAM; SSF56042; PurM C-terminal domain-like; 2.
DR SUPFAM; SSF55326; PurM N-terminal domain-like; 2.
DR PROSITE; PS51273; GATASE_TYPE_1; 1.
PE 4: Predicted;
KW Glutamine amidotransferase {ECO:0000256|PROSITE-ProRule:PRU00605};
KW Ligase {ECO:0000313|EMBL:AFA48415.1};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Purine biosynthesis {ECO:0000256|ARBA:ARBA00022755};
KW Reference proteome {ECO:0000313|Proteomes:UP000007177}.
FT DOMAIN 177..226
FT /note="Phosphoribosylformylglycinamidine synthase linker"
FT /evidence="ECO:0000259|Pfam:PF18072"
FT DOMAIN 441..592
FT /note="PurM-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02769"
FT ACT_SITE 1082
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
FT ACT_SITE 1212
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
FT ACT_SITE 1214
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
SQ SEQUENCE 1241 AA; 136176 MW; D9D3E24DBD00C5FD CRC64;
MIKRIFVEKK DKYQVEAKSL KQTFQRILKI QGLESMRILY RYDVEGVKED LFKQIIGTIL
SEPNVDKVFE DSIQIEEGDK IFGISYLPGQ YDQHGDSAVQ CIQIVSGQRA LVKVAKIVIL
TGALTQTEFD KIKHYMINPV DSQETSLEPF ETLTDEIREP QNILPLVGFI TLTSDQLEAF
RQDNGFAMTT EDLQFVQNYF NNDEKRNPTI TELKVIDTYW SDHCRHTTFL TQIQQVTFLG
DDPISQEMKQ AYDDYSQART ELYGKNTNRP ISLMDLAVMG TKILKKQERI LDLDESEEIN
ACSINIVVDH DGVDENWLLM FKNETHNHPT EIEPFGGAAT CLGGAIRDPL SGRSYVYQAM
RVTGAWDPRS AIEDTLPGKL PQRKISTEAA HGYSSYGNQI GLATGQVTEI YDPGFLAKRM
EVGAVIAATP KENVIRERPV PGDVVLLVGG RTGRDGCGGA TGSSKAHTEE SILESGAEVQ
KGNPVEERKI QRLFRNKKLA NLIKRCNDFG AGGVSVAIGE LADSLEIDLD QVPKKYEGLD
GTELAISESQ ERMAVVVEAQ DVETFIAMGN AENLEVTPVA VVTDSGRLVM KWREAEILNI
SRAFLETNGA PQFIDVVVEA PHKITEAKTA VFIDFKSKML ETLKDLNVAS QRGMVEMFDS
TIGAGTVAMP YGGKNALSPM DGMVAKIPMC EGDTTTCSMM TFGYNPVIAK QSPYLGGMMG
VLESLSKLAA MGGDYKQARL SFQEYFERLG TEPVKWGRPF AALLGAYQVQ TALGTPAIGG
KDSMSGTFGE LTVPPTLISF AVATAHVEAV ITSEIKSTES LIQLFTLPLD DNGVPELTVV
KAVYDILIKA NQEGKILSAK TVGLGGLAEA VAKMSFGNGI GVTLNDHIEL SFLFKPLYGG
ILIESFEILE GSTPIGKTNN KGEIIYAHEA ININELVEAW ETPLRSVYPE KVECQGDVKT
LSYEQTPFIY SKAKILKPQV FIPVFPGTNC EYDSVKAFEN AGAKAVTTIF RNQTVQDISQ
SIDEMVATIK ASQMIMIPGG FSAGDQPNGS GKFIAAVFRN PKMMDAVMEL LNKRDGLVLG
ICNGFQALIK LGLVPNGEIC EITDAMPTLT FNTIGRHVST IPMTRISSNL SPWLANTEVG
DVYRMPMSHG EGRFVASDEV LETLIKNGQI ATQYVDFEGN ATLDGRFNPN GSVYGVEGIT
SLDGRVLGKM GHSERIGKNL YKNIPGNMDQ QIFKAGVNYF K
//
