ID H6NA54_9BACL Unreviewed; 530 AA.
AC H6NA54;
DT 18-APR-2012, integrated into UniProtKB/TrEMBL.
DT 18-APR-2012, sequence version 1.
DT 24-JAN-2024, entry version 42.
DE SubName: Full=Xylan beta-1,4-xylosidase {ECO:0000313|EMBL:AFC28898.1};
GN ORFNames=PM3016_1998 {ECO:0000313|EMBL:AFC28898.1};
OS Paenibacillus mucilaginosus 3016.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=1116391 {ECO:0000313|EMBL:AFC28898.1, ECO:0000313|Proteomes:UP000007523};
RN [1] {ECO:0000313|EMBL:AFC28898.1, ECO:0000313|Proteomes:UP000007523}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3016 {ECO:0000313|EMBL:AFC28898.1,
RC ECO:0000313|Proteomes:UP000007523};
RX PubMed=22535950; DOI=10.1128/JB.00323-12;
RA Ma M., Wang Z., Li L., Jiang X., Guan D., Cao F., Chen H., Wang X.,
RA Shen D., Du B., Li J.;
RT "Complete Genome Sequence of Paenibacillus mucilaginosus 3016, a Bacterium
RT Functional as Microbial Fertilizer.";
RL J. Bacteriol. 194:2777-2778(2012).
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
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DR EMBL; CP003235; AFC28898.1; -; Genomic_DNA.
DR RefSeq; WP_014369345.1; NC_016935.1.
DR AlphaFoldDB; H6NA54; -.
DR STRING; 1116391.PM3016_1998; -.
DR KEGG; pmq:PM3016_1998; -.
DR HOGENOM; CLU_016508_2_1_9; -.
DR Proteomes; UP000007523; Chromosome.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd09000; GH43_SXA-like; 1.
DR Gene3D; 2.60.120.200; -; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR041542; GH43_C2.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR42812; BETA-XYLOSIDASE; 1.
DR PANTHER; PTHR42812:SF12; BETA-XYLOSIDASE-RELATED; 1.
DR Pfam; PF17851; GH43_C2; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361187};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361187};
KW Reference proteome {ECO:0000313|Proteomes:UP000007523}.
FT DOMAIN 327..525
FT /note="Beta-xylosidase C-terminal Concanavalin A-like"
FT /evidence="ECO:0000259|Pfam:PF17851"
FT ACT_SITE 17
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 189
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 130
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 530 AA; 59602 MW; 3268932762C43D9D CRC64;
MSTGNIANPI LAGFQPDASI LRVGDDYYIA TSTFEWFPGV VIHHSKDLVH WRTLTRALDR
VSQLDLLGAP SSGGIWAPAL SYDNGTFYLC ITDVKARRGV YKDLHNYIVS APSITGPWSD
PVYLNSSGFD HFLFHDTDGR KWLFNMQWDF RKNKKRFAGI VMQEYSVAER KLIGPVHKVT
EGTDLGVTEG PMVYKRGGYY YLLLAEGGTG INHAATLMRS RSITGPYEVD PDYPVLTTAG
HPEYPLQKAG HGSLVETQNG EWYMVHICSR QLPDGSGLSP LGRETAIQKV VWTEDGWLRL
AAGGRLPQLE APAPDLPPYP FPAEPARDEF GTAELGVHWH TLRVPADDSW LSLTARPGYL
RLTGRESLNS WHRQSLVARR IGSFRCSVET CVEFEPECYT QMAGLVLYYD EQDHFYLRIS
HDETYGKHLA VVTSERGQYD EAEDYVSVEG WGRCYLRAVI DNENTRFSYS GDGSDWKSIG
PALYTGQLAD EYQRKLSFTG AMAGVCVQDL RGTALHADFD YFEYREHETL
//
