ID   H8GFT2_9PSEU            Unreviewed;       728 AA.
AC   H8GFT2;
DT   16-MAY-2012, integrated into UniProtKB/TrEMBL.
DT   16-MAY-2012, sequence version 1.
DT   27-MAR-2024, entry version 55.
DE   RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE            EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN   ORFNames=SacazDRAFT_04271 {ECO:0000313|EMBL:EHY91117.1};
OS   Saccharomonospora azurea NA-128.
OC   Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC   Pseudonocardiaceae; Saccharomonospora.
OX   NCBI_TaxID=882081 {ECO:0000313|EMBL:EHY91117.1};
RN   [1] {ECO:0000313|EMBL:EHY91117.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NA-128 {ECO:0000313|EMBL:EHY91117.1};
RX   PubMed=22768365; DOI=10.4056/sigs.2635833;
RA   Klenk H.P., Held B., Lucas S., Lapidus A., Copeland A., Hammon N.,
RA   Pitluck S., Goodwin L.A., Han C., Tapia R., Brambilla E.M., Potter G.,
RA   Land M., Ivanova N., Rohde M., Goker M., Detter J.C., Kyrpides N.C.,
RA   Woyke T.;
RT   "Genome sequence of the soil bacterium Saccharomonospora azurea type strain
RT   (NA-128(T)).";
RL   Stand. Genomic Sci. 6:220-229(2012).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC         Evidence={ECO:0000256|ARBA:ARBA00034618};
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DR   EMBL; CM001466; EHY91117.1; -; Genomic_DNA.
DR   RefSeq; WP_005444908.1; NZ_CM001466.1.
DR   AlphaFoldDB; H8GFT2; -.
DR   HOGENOM; CLU_023846_0_0_11; -.
DR   OrthoDB; 3196525at2; -.
DR   Proteomes; UP000004705; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR   Gene3D; 3.30.2310.20; RelE-like; 1.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR035093; RelE/ParE_toxin_dom_sf.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR   PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   SUPFAM; SSF143011; RelE-like; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
PE   4: Predicted;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW   ProRule:PRU00560};
KW   Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW   ProRule:PRU00560};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU00560};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW   ProRule:PRU00560}.
FT   DOMAIN          249..548
FT                   /note="UvrD-like helicase ATP-binding"
FT                   /evidence="ECO:0000259|PROSITE:PS51198"
FT   BINDING         270..277
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ   SEQUENCE   728 AA;  81613 MW;  5E613A17D3871158 CRC64;
     MTVDSGATLR ILERADKEIL KLSRADKGAV YEFQHKFRRN PNHPGLHLKQ LKGDTRLWSA
     RVTQDYRALL LSIQDQDFLL VSVRHRKNAY DDLERYTYRI NGVTGGIEVI DLAAIGDSIL
     GRVAGDGAEP EQAADAAPPL FAHVTDEQFE ELGVAPALFP AIAKVTTEEE LLELADCVPQ
     LTADVLLALY DGKTFDEVME QITAPVAVGN PVDTEDYAAA VVRPATQVTT DDAALQAVLA
     ESFARWQVYL HPTQRTLVEK NYNGPARVSG GPGTGKTIVA LHRVKHLAEQ LPEGRDKPIL
     LTTFNRNLAA DLRTRLLALG GEQLARRVDI VNIDKLASRV VAEGNDDTKR RIVGNDRALE
     EWRSLLLELD DQRFPPEFLA AEWAQVILGQ ALTSRTEYFR ARRTGRGRSL KRDERDHIWQ
     LVERFTQRLD EQGMWTMRQV AAAAARRELD RAARVEHAAA NGENSLSTPE YRYRHIVVDE
     AQDLSAAHWT MLRAMVAKGP NDIFITGDPH QRLYDNYVTL SSLGINIRGR SAKLTLSYRT
     TRQILRWSMG MLTGETYDDL DGGEDDLTGY RSLLNGRDPV AHGLSTWAEE RRWVAQQIKQ
     WQDDDAGSIA IAVPTRQMAD EMIRDLTDAG IDAVQVGPDG PARGDGFHVG TMHRFKGLEY
     QRMILSGVAD GILPRQAIRH FQDSDPKRYQ QERARERSLL FVAATRARDE LVVTWHGKAS
     EFLNQEGD
//