ID H8GQ91_METAL Unreviewed; 789 AA.
AC H8GQ91;
DT 16-MAY-2012, integrated into UniProtKB/TrEMBL.
DT 16-MAY-2012, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=Metal_0715 {ECO:0000313|EMBL:EIC28550.1};
OS Methylomicrobium album BG8.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Methylococcales;
OC Methylococcaceae; Methylomicrobium.
OX NCBI_TaxID=686340 {ECO:0000313|EMBL:EIC28550.1, ECO:0000313|Proteomes:UP000005090};
RN [1] {ECO:0000313|EMBL:EIC28550.1, ECO:0000313|Proteomes:UP000005090}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BG8 {ECO:0000313|EMBL:EIC28550.1,
RC ECO:0000313|Proteomes:UP000005090};
RX PubMed=23580712;
RA Kits K.D., Kalyuzhnaya M.G., Klotz M.G., Jetten M.S., Op den Camp H.J.,
RA Vuilleumier S., Bringel F., Dispirito A.A., Murrell J.C., Bruce D.,
RA Cheng J.F., Copeland A., Goodwin L., Hauser L., Lajus A., Land M.L.,
RA Lapidus A., Lucas S., Medigue C., Pitluck S., Woyke T., Zeytun A.,
RA Stein L.Y.;
RT "Genome Sequence of the Obligate Gammaproteobacterial Methanotroph
RT Methylomicrobium album Strain BG8.";
RL Genome Announc. 1:E0017013-E0017013(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; CM001475; EIC28550.1; -; Genomic_DNA.
DR RefSeq; WP_005369675.1; NZ_CM001475.1.
DR AlphaFoldDB; H8GQ91; -.
DR STRING; 686340.Metal_0715; -.
DR REBASE; 62992; M.MalBG8ORF715P.
DR eggNOG; COG0286; Bacteria.
DR HOGENOM; CLU_012122_0_0_6; -.
DR Proteomes; UP000005090; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF3; TYPE I RESTRICTION ENZYME MJAVIII METHYLASE SUBUNIT; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:EIC28550.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000005090};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:EIC28550.1}.
FT DOMAIN 9..149
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 177..490
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 789 AA; 89519 MW; BA188710CB1326A6 CRC64;
MNHAAHNKLV SFIWSIADDC LRDVYVRGKY RDVILPMVVL RRLDALLEPS KDKVLGELAF
QKNDMGLTEL DDNGLKEASG YVFYNTSKWT LTQLLKTATN NQQILLANVE DYLNGYSANV
KEIVDKFNLK SQVRHMAGKD VLLGVLEKFT SPYINLTPYD KGDPDGNRLP ALTNLGMGYV
FEELIRKFNE ENNEEAGEHF TPREVIELMT HLIFDPVKDQ LPPVMTIYDP ACGSGGMLTE
SQNFIKDEEG AIRATGDVYL YGKEINDETY AICKSDMMIK GNNPANIRVG STLSSDEFSG
TRFDFMLSNP PYGKSWASEQ KYIKDGGDVI DSRFRVTLKD YWGKPETLDA TPRSSDGQLL
FLMEMVSKMK SLDQSPHGSR IASVHNGSSL FTGDAGSGES NIRRHIIEND LLEAIIQLPN
NLFYNTGITT YIWLLSNRKA PSRKNKVQLT DASQLYRKLR KNLGNKNCEF APEHIREIVD
TYLELVTKER ETDDSGIAAQ VFDNSDFGYY KVNIERPDRR KAQFSLERIE TLRFDKALNQ
PMQWIYRQWG DAVYQAGWLG KHEKTILAWC EDNELGLNAK NRGKLLSLDT WLKQQNLMQT
AQTLMAAIGT DESSDFNQFK SSVDAVLKAQ TIKLTASDKN AILNAVSWYD ETAEKVIAQQ
LKLSGDKLDA LLHHLDCSED GLPDFGYYPT GKKGEYLTYE THADLRDSES VPLNDRIHRY
FLAEVKPHVE EAWINLDSTK IGYEISFNKY FYRHKPLRGM DEVAKDIIAL EKQAEGLIAD
ILGIDVNLL
//
