ID   H9D2I1_MOMCH            Unreviewed;       719 AA.
AC   H9D2I1;
DT   16-MAY-2012, integrated into UniProtKB/TrEMBL.
DT   16-MAY-2012, sequence version 1.
DT   24-JAN-2024, entry version 42.
DE   RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
DE            EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
OS   Momordica charantia (Bitter gourd) (Balsam pear).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Momordiceae; Momordica.
OX   NCBI_TaxID=3673 {ECO:0000313|EMBL:AFD54987.1};
RN   [1] {ECO:0000313|EMBL:AFD54987.1}
RP   NUCLEOTIDE SEQUENCE.
RC   TISSUE=Fruit {ECO:0000313|EMBL:AFD54987.1};
RA   Gao S., Li Y.Z., Pan D.M., Chen G.X., Chen Y.Z.;
RT   "Cloning and expressing analysis of beta-galactosidase gene in bitter
RT   gourd.";
RL   Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC         in beta-D-galactosides.; EC=3.2.1.23;
CC         Evidence={ECO:0000256|ARBA:ARBA00001412,
CC         ECO:0000256|RuleBase:RU000675};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC       {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
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DR   EMBL; JQ581596; AFD54987.1; -; mRNA.
DR   AlphaFoldDB; H9D2I1; -.
DR   GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR048913; BetaGal_gal-bd.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR041392; GHD.
DR   InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR   InterPro; IPR019801; Glyco_hydro_35_CS.
DR   InterPro; IPR001944; Glycoside_Hdrlase_35.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR23421:SF63; BETA-GALACTOSIDASE 12; 1.
DR   PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR   Pfam; PF21467; BetaGal_gal-bd; 2.
DR   Pfam; PF17834; GHD; 1.
DR   Pfam; PF01301; Glyco_hydro_35; 1.
DR   PRINTS; PR00742; GLHYDRLASE35.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF49785; Galactose-binding domain-like; 2.
DR   PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE   2: Evidence at transcript level;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU000675};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000675};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           21..719
FT                   /note="Beta-galactosidase"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5003618585"
FT   DOMAIN          29..332
FT                   /note="Glycoside hydrolase 35 catalytic"
FT                   /evidence="ECO:0000259|Pfam:PF01301"
FT   DOMAIN          355..411
FT                   /note="Beta-galactosidase beta-sandwich"
FT                   /evidence="ECO:0000259|Pfam:PF17834"
FT   DOMAIN          462..529
FT                   /note="Beta-galactosidase galactose-binding"
FT                   /evidence="ECO:0000259|Pfam:PF21467"
FT   DOMAIN          611..697
FT                   /note="Beta-galactosidase galactose-binding"
FT                   /evidence="ECO:0000259|Pfam:PF21467"
SQ   SEQUENCE   719 AA;  80354 MW;  439F01BA480CE2A1 CRC64;
     MSKCVLLFLG LLSWVCYAMA TVTYDQKAII INGKRRILVS GSIHYPRSTP QMWPSLIQNA
     KDGGLDIIET YVFWNGHEPT QGKYYFEDRY DLVRFIKLVQ QAGLYVHLRI GPYVCAEWNY
     GGFPIWLKHV PGIVFRTENE PFKAAMQKFT EKIVGMMKSE KLYESQGGPI ILSQIENEYG
     PVEWEIGAPG KSYTKWAAQM ALGLDTGVPW VMCKQEDAPD PVIDTCNGFY CENFKPNREN
     KPKIWTEVWS GWYTAFGGAV PYRPAEDLAF SVARFVQNGG SLFNYYMYHG GTNFGRSSGL
     FIANSYDFDA PIDEYGLKRE PKWEHLRDLH KAIKLCEPAL VSADPNVTWL GKNLEARVFK
     SSSGACAAFL ANYDISTSSK VSFWNTQYDL PPWSISILSD CKSAIFNTAR IGAQSAPMKM
     MLVSSFWWLS YKEEVASGYA TDTTTKDGLV EQVNFTWDST DYLWYMTDIQ IDPNEAFIKS
     GQWPLLNISS AGHVLHVFVN GQLSGTVYGS LENPKVAFSK YVNLKAGVNK LSMLSVTVGL
     PNVGLHFESW NAGVLGPVTL KGLNEGIRDM SGYKWSHKVG LKGENMNLHT IGGSNSVQWA
     KGSGLVQKQP LTWYKTNFNT PAGNEPLALD MSSMGKGQIW INGRSIGRYW PAYAASGSCG
     KCSYAGIFTE KKCLSNCGQP SQKWYHVPRE WLESKGNFLV VFEELGGNPG GISLVKRSP
//