ID H9G6K0_ANOCA Unreviewed; 890 AA.
AC H9G6K0;
DT 16-MAY-2012, integrated into UniProtKB/TrEMBL.
DT 29-SEP-2021, sequence version 2.
DT 27-MAR-2024, entry version 61.
DE RecName: Full=alpha-1,2-Mannosidase {ECO:0000256|RuleBase:RU361193};
DE EC=3.2.1.- {ECO:0000256|RuleBase:RU361193};
GN Name=EDEM3 {ECO:0000313|Ensembl:ENSACAP00000002602.3};
OS Anolis carolinensis (Green anole) (American chameleon).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC Iguania; Dactyloidae; Anolis.
OX NCBI_TaxID=28377 {ECO:0000313|Ensembl:ENSACAP00000002602.3, ECO:0000313|Proteomes:UP000001646};
RN [1] {ECO:0000313|Ensembl:ENSACAP00000002602.3}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JBL SC #1 {ECO:0000313|Ensembl:ENSACAP00000002602.3};
RG The Genome Sequencing Platform;
RA Di Palma F., Alfoldi J., Heiman D., Young S., Grabherr M., Johnson J.,
RA Lander E.S., Lindblad-Toh K.;
RT "The Genome Sequence of Anolis carolinensis (Green Anole Lizard).";
RL Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSACAP00000002602.3}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|PIRSR:PIRSR601382-2};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 47 family.
CC {ECO:0000256|ARBA:ARBA00007658, ECO:0000256|RuleBase:RU361193}.
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DR AlphaFoldDB; H9G6K0; -.
DR STRING; 28377.ENSACAP00000002602; -.
DR Ensembl; ENSACAT00000002668.4; ENSACAP00000002602.3; ENSACAG00000002542.4.
DR eggNOG; KOG2430; Eukaryota.
DR GeneTree; ENSGT00940000159391; -.
DR InParanoid; H9G6K0; -.
DR TreeFam; TF300807; -.
DR Proteomes; UP000001646; Unplaced.
DR Bgee; ENSACAG00000002542; Expressed in testis and 13 other cell types or tissues.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; IEA:InterPro.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0004571; F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity; IBA:GO_Central.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0036503; P:ERAD pathway; IEA:Ensembl.
DR GO; GO:0006058; P:mannoprotein catabolic process; IEA:Ensembl.
DR CDD; cd02126; PA_EDEM3_like; 1.
DR Gene3D; 1.50.10.10; -; 1.
DR Gene3D; 3.50.30.30; -; 1.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR044674; EDEM1/2/3.
DR InterPro; IPR037322; EDEM3_PA.
DR InterPro; IPR001382; Glyco_hydro_47.
DR InterPro; IPR046450; PA_dom_sf.
DR InterPro; IPR003137; PA_domain.
DR InterPro; IPR036026; Seven-hairpin_glycosidases.
DR PANTHER; PTHR45679; ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2; 1.
DR PANTHER; PTHR45679:SF2; ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 3; 1.
DR Pfam; PF01532; Glyco_hydro_47; 1.
DR Pfam; PF02225; PA; 1.
DR PRINTS; PR00747; GLYHDRLASE47.
DR SUPFAM; SSF52025; PA domain; 1.
DR SUPFAM; SSF48225; Seven-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|PIRSR:PIRSR601382-2};
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Glycosidase {ECO:0000256|RuleBase:RU361193};
KW Hydrolase {ECO:0000256|RuleBase:RU361193};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR601382-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000001646}.
FT DOMAIN 654..742
FT /note="PA"
FT /evidence="ECO:0000259|Pfam:PF02225"
FT REGION 767..875
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 767..787
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 810..870
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 114
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR601382-1"
FT ACT_SITE 261
FT /evidence="ECO:0000256|PIRSR:PIRSR601382-1"
FT ACT_SITE 355
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR601382-1"
FT ACT_SITE 373
FT /evidence="ECO:0000256|PIRSR:PIRSR601382-1"
FT BINDING 459
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR601382-2"
SQ SEQUENCE 890 AA; 100235 MW; 90C107548EA90805 CRC64;
MASLLLCHMI YLSTHLFSFP FTCSLFVCWF IACNQQEHAY PADELMPLTC RGRVRGQEPS
RGDVDDALGK FSLTLIDTLD TLVVLNKTKE FEDAVKKVIR DVNLDNDIVV SVFETNIRVL
GGLLGGHSVA IMLKEKGEVM QWYSGELLHM AKLLGYKLLP AFNTTSGLPY PRVNLKFGVR
SPEARTGTET DTCTACAGTL ILEFAALSRF TGASIFEEYA RKALDFLWEK RQRSSNLVGV
TINIHTGDWV RKDSGVGAGI DSYYEYLLKA YVLLGDDSFL ERFNTHYDAI MRYISQPPLL
LDVHIHKPML NARTWMDSLL AFFPGLQVLK GDIRPAIETH EMLYQVIKKH NFLPEAFTTD
FRVHWAQHPL RPEFAESTYF LYKATGDPYY LEVGKTLIEN LNKYARVPCG FAAMKDVRTG
SHEDRMDSFF LAEMFKYLYL LFTEKEDLTF DIEDYIFTTE AHLLPLWLST TNQTAPRKNI
TTEYTELDDS NFDWTCPNTQ ILFPNDPMYA QSIREPLKNV VDKSCPRSIS RVEESLGNGP
KPPLRARDFM ASNPEHLEIL KKMGVSLIHL KDGRVQLVQH AIQAASSLDA EDGLRFMQEM
IELSSQQQKE QQLPPRAVQI VSHPFFGRVV LTAGPAQFGM DLSKHKSGTR GFVAVSKPYN
GCSEITNPES LKEKIALMQR GQCMFAEKAR NIQKAGAIGG IVIDDNEGSS SDTAPLFQMA
GDGKNTEDIT IPMLFLFNKE GSIILDAIQE YEAVEVLLSD KAKDRDLEME SMDQKSSDND
SHNQRSEEIS TDLNLVPQDP EEGGLSSHAE LDSSRVSLSD EDSCIPKLQE TSSTNSPVSE
LDNPLEEQSK TETDSSPSNS WDNKGQSMDS ILADWNEDIE AFEMMEKDEL
//
