ID HCBT3_DIACA Reviewed; 445 AA.
AC O23918;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 13-SEP-2023, entry version 67.
DE RecName: Full=Anthranilate N-benzoyltransferase protein 3;
DE EC=2.3.1.144;
DE AltName: Full=Anthranilate N-hydroxycinnamoyl/benzoyltransferase 3;
GN Name=HCBT3;
OS Dianthus caryophyllus (Carnation) (Clove pink).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC Caryophyllales; Caryophyllaceae; Caryophylleae; Dianthus.
OX NCBI_TaxID=3570;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 7-18; 27-45; 74-84;
RP 204-209; 299-306; 343-368; 373-385 AND 441-445, AND FUNCTION.
RX PubMed=9426598; DOI=10.1023/a:1005878622437;
RA Yang Q., Reinhard K., Schiltz E., Matern U.;
RT "Characterization and heterologous expression of hydroxycinnamoyl/benzoyl-
RT CoA:anthranilate N-hydroxycinnamoyl/benzoyltransferase from elicited cell
RT cultures of carnation, Dianthus caryophyllus L.";
RL Plant Mol. Biol. 35:777-789(1997).
RN [2]
RP INDUCTION.
RX PubMed=9869425; DOI=10.1023/a:1006003731919;
RA Yang Q., Grimmig B., Matern U.;
RT "Anthranilate N-hydroxycinnamoyl/benzoyltransferase gene from carnation:
RT rapid elicitation of transcription and promoter analysis.";
RL Plant Mol. Biol. 38:1201-1214(1998).
CC -!- FUNCTION: Catalyzes the formation of N-benzoylanthranilate, in the
CC course of methoxydianthramide B, a phytoalexin. Phytoalexins are
CC produced in response to infection by parasites, and are essential for
CC the expression of disease resistance. {ECO:0000269|PubMed:9426598}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=anthranilate + benzoyl-CoA = CoA + N-benzoylanthranilate;
CC Xref=Rhea:RHEA:21600, ChEBI:CHEBI:16567, ChEBI:CHEBI:17331,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57369; EC=2.3.1.144;
CC -!- PATHWAY: Phytoalexin biosynthesis; methoxydianthramide B biosynthesis.
CC -!- INDUCTION: By fungal elicitors. Elicitation triggers a rapid, transient
CC induction, reaching maximal abundances within about 0.5 hours and
CC returning to basal levels within 4 hours. {ECO:0000269|PubMed:9869425}.
CC -!- PTM: N-terminus is blocked. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR EMBL; Z84571; CAB06538.1; -; mRNA.
DR PIR; T10719; T10719.
DR AlphaFoldDB; O23918; -.
DR SMR; O23918; -.
DR BioCyc; MetaCyc:MONOMER-15062; -.
DR UniPathway; UPA00900; -.
DR GO; GO:0047672; F:anthranilate N-benzoyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 2.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR PANTHER; PTHR31642:SF11; SHIKIMATE O-HYDROXYCINNAMOYLTRANSFERASE; 1.
DR PANTHER; PTHR31642; TRICHOTHECENE 3-O-ACETYLTRANSFERASE; 1.
DR Pfam; PF02458; Transferase; 1.
PE 1: Evidence at protein level;
KW Acyltransferase; Direct protein sequencing; Flavonoid biosynthesis;
KW Transferase.
FT CHAIN 1..445
FT /note="Anthranilate N-benzoyltransferase protein 3"
FT /id="PRO_0000147365"
FT ACT_SITE 164
FT /note="Proton acceptor"
FT /evidence="ECO:0000255"
FT ACT_SITE 392
FT /note="Proton acceptor"
FT /evidence="ECO:0000255"
SQ SEQUENCE 445 AA; 50149 MW; BE3C82F236CFDD7A CRC64;
MSIHIKQSTM VRPAEETPNK SLWLSKIDMI LRTPYSHTGA VLIYKQPDNN EDNIQPSSSM
YFDANILIEA LSKALVPYYP MAGRLKINGD RYEIDCNGEG ALFVEAESSH VLEDFGDFRP
NDELHRVMVP TCDYSKGISS FPLLMVQLTR FRCGGVSIGF AQHHHVCDRM SHFEFNNSWA
RIAKGLLPAL EPVHDRYLHL CPRNPPQIKY THSQFEPFVP SLPKELLDGK TSKSQTLFKL
SREQINTLKQ KLDWSNTTTR LSTYEVVAGH VWRSVSKARG LSDHEEIKLI MPVDGRSRIN
NPSLPKGYCG NVVFLAVCTA TVGDLACNPL TDTAGKVQEA LKGLDDDYLR SAIDHTESKP
DLPVPYMGSP EKTLYPNVLV NSWGRIPYQA MDFGWGNPTF FGISNIFYDG QCFLIPSQNG
DGSMTLAINL FSSHLSLFKK HFYDF
//
