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Database: UniProt
Entry: HM34_CAEEL
LinkDB: HM34_CAEEL
Original site: HM34_CAEEL 
ID   HM34_CAEEL              Reviewed;         256 AA.
AC   Q94165;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 2.
DT   27-MAR-2024, entry version 143.
DE   RecName: Full=Homeobox protein ceh-34;
GN   Name=ceh-34; ORFNames=C10G8.6;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [2]
RP   FUNCTION, INTERACTION WITH EYA-1, SUBCELLULAR LOCATION, DEVELOPMENTAL
RP   STAGE, AND DISRUPTION PHENOTYPE.
RX   PubMed=19427847; DOI=10.1016/j.ydbio.2009.05.538;
RA   Amin N.M., Lim S.E., Shi H., Chan T.L., Liu J.;
RT   "A conserved Six-Eya cassette acts downstream of Wnt signaling to direct
RT   non-myogenic versus myogenic fates in the C. elegans postembryonic
RT   mesoderm.";
RL   Dev. Biol. 331:350-360(2009).
RN   [3]
RP   FUNCTION, AND DEVELOPMENTAL STAGE.
RX   PubMed=20335356; DOI=10.1242/dev.048496;
RA   Amin N.M., Shi H., Liu J.;
RT   "The FoxF/FoxC factor LET-381 directly regulates both cell fate
RT   specification and cell differentiation in C. elegans mesoderm
RT   development.";
RL   Development 137:1451-1460(2010).
RN   [4]
RP   FUNCTION, INTERACTION WITH EYA-1, SUBCELLULAR LOCATION, DEVELOPMENTAL
RP   STAGE, AND DISRUPTION PHENOTYPE.
RX   PubMed=20713707; DOI=10.1073/pnas.1010023107;
RA   Hirose T., Galvin B.D., Horvitz H.R.;
RT   "Six and Eya promote apoptosis through direct transcriptional activation of
RT   the proapoptotic BH3-only gene egl-1 in Caenorhabditis elegans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 107:15479-15484(2010).
CC   -!- FUNCTION: Acts as a transcription regulator (PubMed:20713707). Binds to
CC       the sequence motif 5'-TCAGGTT-3' (PubMed:20335356). Binds to the cis-
CC       regulatory element of proapoptotic factor egl-1 gene and together with
CC       eya-1 activates egl-1 expression to promote motor neuron M4 sister cell
CC       apoptosis (PubMed:20713707). Also promotes apoptosis of I1 pharyngeal
CC       neuron sister cell (PubMed:20713707). Together with eya-1, required to
CC       specify the coelomocyte fate in embryonic and postembryonic precursors
CC       (PubMed:19427847, PubMed:20335356). {ECO:0000269|PubMed:19427847,
CC       ECO:0000269|PubMed:20335356, ECO:0000269|PubMed:20713707}.
CC   -!- SUBUNIT: Interacts (via N-terminus) with eya-1 (via C-terminus).
CC       {ECO:0000269|PubMed:19427847, ECO:0000269|PubMed:20713707}.
CC   -!- INTERACTION:
CC       Q94165; O17670: eya-1; NbExp=3; IntAct=EBI-2413136, EBI-311862;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19427847,
CC       ECO:0000269|PubMed:20713707}.
CC   -!- DEVELOPMENTAL STAGE: Expression starts at the embryonic bean stage and
CC       continues throughout larval and adult stages in few anterior body wall
CC       muscle cells and in unidentified cells in the head (PubMed:19427847).
CC       Expressed predominantly in pharyngeal cells during embryogenesis and
CC       throughout larval and adult stages (PubMed:20713707). In L1 larvae,
CC       expressed in all pharyngeal neurons (M4, I1, MI, I3, M3, NSM, MC, I2,
CC       I4, I5, I6, M1, M2, and M5), some pharyngeal muscle cells (pm1 and pm2)
CC       and pharyngeal epithelial cells (e1 and e3), and some body wall muscles
CC       around the anterior pharynx (PubMed:20713707). Expressed transiently in
CC       the M lineage-derived coelomocyte precursor cells M.dlpa and M.drpa
CC       before their differentiation into coelomocytes (PubMed:19427847,
CC       PubMed:20335356). Expressed in the M4 motor neuron sister cell
CC       (PubMed:20713707). {ECO:0000269|PubMed:19427847,
CC       ECO:0000269|PubMed:20335356, ECO:0000269|PubMed:20713707}.
CC   -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown in embryos causes lethal
CC       arrest at the embryonic 3-fold stage or at the L1 larval stage with
CC       abnormal anterior morphology (PubMed:19427847). The few surviving
CC       animals lack 1 or both pairs of embryonic MS lineage-derived
CC       coelomocytes as well as the 2 post embryonic M lineage-derived
CC       coelomocytes (PubMed:19427847). RNAi-mediated knockdown in L1 larvae
CC       causes the loss of the M lineage-derived coelomocytes due to the
CC       abnormal differentiation of coelomocyte precursors M.drpa and M.dlpa
CC       into body wall muscle cells (PubMed:19427847). In 38 percent of
CC       animals, the motor neuron M4 sister survived (PubMed:20713707).
CC       {ECO:0000269|PubMed:19427847, ECO:0000269|PubMed:20713707}.
CC   -!- SIMILARITY: Belongs to the SIX/Sine oculis homeobox family.
CC       {ECO:0000305}.
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DR   EMBL; FO080497; CCD64164.1; -; Genomic_DNA.
DR   PIR; F89046; F89046.
DR   RefSeq; NP_504419.1; NM_072018.6.
DR   AlphaFoldDB; Q94165; -.
DR   SMR; Q94165; -.
DR   BioGRID; 43968; 2.
DR   IntAct; Q94165; 2.
DR   STRING; 6239.C10G8.6.1; -.
DR   PaxDb; 6239-C10G8-6; -.
DR   EnsemblMetazoa; C10G8.6.1; C10G8.6.1; WBGene00000455.
DR   GeneID; 178919; -.
DR   KEGG; cel:CELE_C10G8.6; -.
DR   UCSC; C10G8.6; c. elegans.
DR   AGR; WB:WBGene00000455; -.
DR   WormBase; C10G8.6; CE20496; WBGene00000455; ceh-34.
DR   eggNOG; KOG0775; Eukaryota.
DR   GeneTree; ENSGT00940000167410; -.
DR   HOGENOM; CLU_046914_4_0_1; -.
DR   InParanoid; Q94165; -.
DR   OMA; YKIMESH; -.
DR   OrthoDB; 602349at2759; -.
DR   PhylomeDB; Q94165; -.
DR   PRO; PR:Q94165; -.
DR   Proteomes; UP000001940; Chromosome V.
DR   Bgee; WBGene00000455; Expressed in pharyngeal muscle cell (C elegans) and 3 other cell types or tissues.
DR   GO; GO:0005634; C:nucleus; IDA:WormBase.
DR   GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR   GO; GO:0007501; P:mesodermal cell fate specification; IMP:WormBase.
DR   GO; GO:1904747; P:positive regulation of apoptotic process involved in development; IMP:UniProtKB.
DR   GO; GO:0006355; P:regulation of DNA-templated transcription; IMP:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00086; homeodomain; 1.
DR   Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR031701; SIX1_SD.
DR   PANTHER; PTHR10390; HOMEOBOX PROTEIN SIX; 1.
DR   PANTHER; PTHR10390:SF61; HOMEOBOX PROTEIN SIX2; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   Pfam; PF16878; SIX1_SD; 1.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
PE   1: Evidence at protein level;
KW   Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..256
FT                   /note="Homeobox protein ceh-34"
FT                   /id="PRO_0000049000"
FT   DNA_BIND        134..193
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          187..233
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        187..203
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        219..233
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   256 AA;  29622 MW;  4283D31FD0A04D7C CRC64;
     MQQSYNPQNS LTATTSYSEQ EIVCICESLF NEGLQTGRTE QLANFIYNLP QCYQVMESVL
     KAQALVYFTT QNWKMLYKLL ECSKFSPHNH TVLQNLWLDA HYKEAAKTKD RELGAVCKYR
     IRKKNPFPNT IWDGEETNYC FKSKSRNVLR DAYKKCQYPS VEDKRRLAQQ TELSIIQVSN
     WFKNKRQRER AAGQLDRSSA RSNDSDDGSS GCESKPPMNI DSPAPPPLPT SFDLQPYYPS
     PYTFAPHCDF SYIQNL
//
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