ID HOOK_DROPE Reviewed; 677 AA.
AC B4G831;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 07-JUL-2009, sequence version 2.
DT 24-JAN-2024, entry version 61.
DE RecName: Full=Protein hook;
GN Name=hook {ECO:0000250|UniProtKB:Q24185};
GN Synonyms=hk {ECO:0000250|UniProtKB:Q24185}; ORFNames=GL19224;
OS Drosophila persimilis (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7234;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MSH-3 / Tucson 14011-0111.49;
RX PubMed=17994087; DOI=10.1038/nature06341;
RG Drosophila 12 genomes consortium;
RT "Evolution of genes and genomes on the Drosophila phylogeny.";
RL Nature 450:203-218(2007).
CC -!- FUNCTION: Involved in endocytic trafficking by stabilizing organelles
CC of the endocytic pathway. Probably acts as a cytoskeletal linker
CC protein required to tether endosome vesicles to the cytoskeleton.
CC Involved in modulation of endocytosis at stages required for down-
CC regulation of membrane proteins that control synapse size. Not involved
CC in synaptic vesicle recycling. Required in R7 cells for boss
CC endocytosis into multivesicular bodies (MVBs). Has a role in regulating
CC adult longevity. {ECO:0000250|UniProtKB:Q24185}.
CC -!- SUBUNIT: Homodimer. Interacts with microtubules via its N-terminus.
CC {ECO:0000250|UniProtKB:Q24185}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000250|UniProtKB:Q24185}. Endosome
CC {ECO:0000250|UniProtKB:Q24185}. Synapse {ECO:0000250|UniProtKB:Q24185}.
CC Note=Enriched at neuromuscular synapses, in both presynaptic and
CC postsynaptic regions. {ECO:0000250|UniProtKB:Q24185}.
CC -!- DOMAIN: The coiled coil domain mediates homodimerization.
CC {ECO:0000250|UniProtKB:Q24185}.
CC -!- SIMILARITY: Belongs to the hook family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EDW28511.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=EDW28511.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; CH479180; EDW28511.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; XP_002014515.1; XM_002014479.1.
DR AlphaFoldDB; B4G831; -.
DR SMR; B4G831; -.
DR STRING; 7234.B4G831; -.
DR eggNOG; ENOG502QQM8; Eukaryota.
DR Proteomes; UP000008744; Unassembled WGS sequence.
DR GO; GO:0005768; C:endosome; ISS:UniProtKB.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0045202; C:synapse; ISS:UniProtKB.
DR GO; GO:0008017; F:microtubule binding; ISS:UniProtKB.
DR GO; GO:0031267; F:small GTPase binding; IEA:EnsemblMetazoa.
DR GO; GO:0031122; P:cytoplasmic microtubule organization; IEA:InterPro.
DR GO; GO:0030705; P:cytoskeleton-dependent intracellular transport; ISS:UniProtKB.
DR GO; GO:0008340; P:determination of adult lifespan; ISS:UniProtKB.
DR GO; GO:0006897; P:endocytosis; ISS:UniProtKB.
DR CDD; cd22222; HkD_Hook; 1.
DR Gene3D; 1.10.418.10; Calponin-like domain; 1.
DR InterPro; IPR001715; CH_dom.
DR InterPro; IPR036872; CH_dom_sf.
DR InterPro; IPR008636; Hook_C.
DR InterPro; IPR043936; HOOK_N.
DR PANTHER; PTHR18947; HOOK PROTEINS; 1.
DR PANTHER; PTHR18947:SF39; PROTEIN HOOK; 1.
DR Pfam; PF05622; HOOK; 1.
DR Pfam; PF19047; HOOK_N; 1.
DR SUPFAM; SSF116907; Hook domain; 1.
DR PROSITE; PS50021; CH; 1.
PE 3: Inferred from homology;
KW Coiled coil; Cytoplasm; Cytoskeleton; Developmental protein; Endocytosis;
KW Endosome; Microtubule; Reference proteome; Synapse.
FT CHAIN 1..677
FT /note="Protein hook"
FT /id="PRO_0000379066"
FT DOMAIN 6..123
FT /note="Calponin-homology (CH)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT COILED 135..436
FT /evidence="ECO:0000255"
FT COILED 478..588
FT /evidence="ECO:0000255"
SQ SEQUENCE 677 AA; 76821 MW; AE45AADAFA97AFCA CRC64;
MSAAKNEMYY SLLEWFKTLN LNAPHADAES LADGVAVAQA LNQFAPESFT DSWLAKIKAS
AVGINWRLRM SNLKKVTQSL YDYYSEVLNY TLSDFVKPDV QRIAEKCDLV ELERLLQLVL
GCAVNCAKKQ SYITEIMCLE EELQANIMRA LQELESSRNA AEGGIVTSLS RSSISGMLDG
KVLQEERDAM AQKCFETEKK MLLLIDEKTN LQQELQRVQK EFARLEHSST VIGDDGVSLG
PVQTGSVRYN ELRRQLDLLK EELLQSEGAR EDLKLKAQQQ ETDLWHMQMR IDELLKSTAE
VTTLKDEVDV LRESNDKLKI CEGQLDTYKK KLEDYNDLKK QVKILEERSA DYVQQNAQFE
EDAKRYANTK GQIELFKKEI QDLHTKLDSE SSKNVKLEFD NKNLEGKNLA LQRAKDSLLK
ERDNLRETVD ELKCGHLSSN SGLTGTTVSR ELQPPATVEK MQRLEAENKA LREGQGGQTA
LAQLLDDANK RCENLREQLK TANERILSLS HASQSDDPIL KESEFGKQIK QLMELNEQKT
LQLEESVTQS SSLQCKVTQL ETNLTAREQE VMAYDAKYRK CLEKAKEVIK SFDPRIASAI
DASALEKYFD VVEEEPKPKM SVMEEQLMTS AFYRLGVNAQ RDAVDSKLAI LMGSGQTFLA
RQRQSAPRKS LSAMKSK
//
