ID I0SYV0_STRMT Unreviewed; 1169 AA.
AC I0SYV0;
DT 13-JUN-2012, integrated into UniProtKB/TrEMBL.
DT 13-JUN-2012, sequence version 1.
DT 24-JAN-2024, entry version 55.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969,
GN ECO:0000313|EMBL:EID28553.1};
GN ORFNames=HMPREF1048_0123 {ECO:0000313|EMBL:EID28553.1};
OS Streptococcus mitis SK575.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=1095736 {ECO:0000313|EMBL:EID28553.1, ECO:0000313|Proteomes:UP000005505};
RN [1] {ECO:0000313|EMBL:EID28553.1, ECO:0000313|Proteomes:UP000005505}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SK575 {ECO:0000313|EMBL:EID28553.1,
RC ECO:0000313|Proteomes:UP000005505};
RA Harkins D.M., Madupu R., Durkin A.S., Torralba M., Methe B., Sutton G.G.,
RA Nelson K.E.;
RL Submitted (FEB-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EID28553.1}.
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DR EMBL; AICU01000054; EID28553.1; -; Genomic_DNA.
DR RefSeq; WP_000212600.1; NZ_AICU01000054.1.
DR AlphaFoldDB; I0SYV0; -.
DR PATRIC; fig|1095736.3.peg.761; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000005505; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}.
FT DOMAIN 627..788
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 809..963
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1169 AA; 134991 MW; 3A600AD88D1DBFBC CRC64;
MATLLDLFSE NDQIQKWHQN LTDKKRQLML GLSTSTKALA IASSLKKEDK IVLLTSTYGE
AEGLVSDLIS ILGEELVYPF LVDDSPMVEF LISSQEKIIS RVEALRFLTD SSKRGILVCN
IAASRLILPS PNVFKDSIVK ISVGEEYDQH ALIHQLKEIG YRKVTQVQTQ GEFSLRGDIL
DIFEISQLEP CRIEFFGDEV DGIRLFEVET QLSKENQTEL TIFPASDVIL REKDYQRGRS
ALEKQVSKTL SPILKSYLEE ILSSFHQKQI HSDSRKFLSL CYDKTWTIFD YIEKDIPIFF
DDYQKLMNQY EVFERELAQY FTEELQNSKA FSEMKYFADI EQIYKKQSPV TFFSNLQKGL
GNLKFDQIYQ FNQYPMQEFF NQFSFLKEEI ERYKKMDYTI ILQSSNSMGS KTLEDVLEEY
QIKLDSRDKS RICQESVNLI EGNLRHGFHF VDEKVLLITE HEIFQKKLKR RFRRQHVSNA
ERLKDYNELE KGDYVVHHIH GIGQYLGIET IEIKGIHRDY VSVQYQNGDQ ISIPVEQIHL
LSKYVSSDGK APKLNKLNDG HFKKAKQKVK NQVEDIADDL IKLYSERSQL KGFAFSADDE
DQDAFDDAFP YVETDDQLRS IEEIKRDMQA SQPMDRLLVG DVGFGKTEVA MRAAFKAVND
HKQVVVLVPT TVLAQQHYTN FKERFQNFAV NIDVLSRFRS KKEQTETLEK LKNGQVDILI
GTHRVLSKDV VFADLGLMII DEEQRFGVKH KETLKELKKQ VDVLTLTATP IPRTLHMSML
GIRDLSVIET PPTNRYPVQT YVLEKNDSVI RDAVLREMER GGQVYYLYNK VDTIDQKVSE
LQELIPEASI GYVHGRMSEI QLENTLLDFI EGQYDILVTT TIIETGVDIP NANTLFIENA
DHMGLSTLYQ LRGRVGRSNR IAYAYLMYRP EKSISEVSEK RLEAIKGFTE LGSGFKIAMR
DLSIRGAGNL LGKSQSGFID SVGFELYSQL LEEAIAKRNG NANANTRTKG NAELILQIDA
YLPDTYISDQ RHKIEIYKKI RQIDNRVNYE ELQEELMDRF GEYPDVVVYL LEIGLVKSYL
DKVFVQRVER KDNKITIQFE KVTQRLFLAQ DYFKALSATN FKAGIAENKG LMELVFDVQN
KKDYEILESL LIFGESLLEI KESKKENSI
//
