ID I1GU88_BRADI Unreviewed; 1267 AA.
AC I1GU88;
DT 13-JUN-2012, integrated into UniProtKB/TrEMBL.
DT 13-JUN-2012, sequence version 1.
DT 27-MAR-2024, entry version 62.
DE SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:KQK16145.1, ECO:0000313|EnsemblPlants:KQK16145};
GN Name=100838876 {ECO:0000313|EnsemblPlants:KQK16145};
GN ORFNames=BRADI_1g27027v3 {ECO:0000313|EMBL:KQK16145.1};
OS Brachypodium distachyon (Purple false brome) (Trachynia distachya).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Stipodae; Brachypodieae; Brachypodium.
OX NCBI_TaxID=15368 {ECO:0000313|EMBL:KQK16145.1};
RN [1] {ECO:0000313|EMBL:KQK16145.1, ECO:0000313|EnsemblPlants:KQK16145}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bd21 {ECO:0000313|EMBL:KQK16145.1,
RC ECO:0000313|EnsemblPlants:KQK16145};
RX PubMed=20148030; DOI=10.1038/nature08747;
RG International Brachypodium Initiative;
RT "Genome sequencing and analysis of the model grass Brachypodium
RT distachyon.";
RL Nature 463:763-768(2010).
RN [2] {ECO:0000313|EMBL:KQK16145.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Bd21 {ECO:0000313|EMBL:KQK16145.1};
RG The International Brachypodium Initiative;
RA Lucas S., Harmon-Smith M., Lail K., Tice H., Grimwood J., Bruce D.,
RA Barry K., Shu S., Lindquist E., Wang M., Pitluck S., Vogel J.P.,
RA Garvin D.F., Mockler T.C., Schmutz J., Rokhsar D., Bevan M.W.;
RT "WGS assembly of Brachypodium distachyon.";
RL Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EnsemblPlants:KQK16145}
RP IDENTIFICATION.
RC STRAIN=cv. Bd21 {ECO:0000313|EnsemblPlants:KQK16145};
RG EnsemblPlants;
RL Submitted (AUG-2018) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00000600};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:13066;
CC Evidence={ECO:0000256|ARBA:ARBA00000600};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the DNA2/NAM7 helicase family.
CC {ECO:0000256|ARBA:ARBA00007913}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU01341}.
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DR EMBL; CM000880; KQK16145.1; -; Genomic_DNA.
DR RefSeq; XP_003563076.1; XM_003563028.3.
DR AlphaFoldDB; I1GU88; -.
DR STRING; 15368.I1GU88; -.
DR EnsemblPlants; KQK16145; KQK16145; BRADI_1g27027v3.
DR GeneID; 100838876; -.
DR Gramene; KQK16145; KQK16145; BRADI_1g27027v3.
DR KEGG; bdi:100838876; -.
DR eggNOG; KOG1802; Eukaryota.
DR HOGENOM; CLU_001666_4_3_1; -.
DR InParanoid; I1GU88; -.
DR OMA; VRSNDNQ; -.
DR OrthoDB; 170190at2759; -.
DR Proteomes; UP000008810; Chromosome 1.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IBA:GO_Central.
DR CDD; cd21407; 1B_UPF1-like; 1.
DR CDD; cd18039; DEXXQc_UPF1; 1.
DR CDD; cd18808; SF1_C_Upf1; 1.
DR CDD; cd21400; ZBD_UPF1-like; 1.
DR Gene3D; 2.40.30.230; -; 1.
DR Gene3D; 6.10.140.1240; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR045055; DNA2/NAM7-like.
DR InterPro; IPR041679; DNA2/NAM7-like_C.
DR InterPro; IPR041677; DNA2/NAM7_AAA_11.
DR InterPro; IPR006935; Helicase/UvrB_N.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047187; SF1_C_Upf1.
DR InterPro; IPR040812; UPF1_1B_dom.
DR InterPro; IPR018999; UPF1_CH/ZBD.
DR PANTHER; PTHR10887; DNA2/NAM7 HELICASE FAMILY; 1.
DR PANTHER; PTHR10887:SF364; REGULATOR OF NONSENSE TRANSCRIPTS 1; 1.
DR Pfam; PF13086; AAA_11; 1.
DR Pfam; PF13087; AAA_12; 1.
DR Pfam; PF04851; ResIII; 1.
DR Pfam; PF18141; UPF1_1B_dom; 1.
DR Pfam; PF09416; UPF1_Zn_bind; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51997; UPF1_CH_RICH; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|PROSITE-
KW ProRule:PRU01341}; Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000008810};
KW Zinc {ECO:0000256|PROSITE-ProRule:PRU01341};
KW Zinc-finger {ECO:0000256|PROSITE-ProRule:PRU01341}.
FT DOMAIN 147..306
FT /note="Upf1"
FT /evidence="ECO:0000259|PROSITE:PS51997"
FT DOMAIN 512..644
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT REGION 1..112
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 217..247
FT /note="C4"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01341"
FT REGION 1218..1242
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 87..110
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1267 AA; 138294 MW; 9E3FC501A7B25F12 CRC64;
MATQPPSSAA AADLYETASQ PDPSASAAGD AYTFLEFNTQ GDDFEYPDFP ELSQPARSAP
PPATVTSSTS SSWPAPPPPP DASQDPDLVP QDSTPLASSS SPSPRSSASK ARASAAVADG
LAAGVAALSF EEPPGAGAGE DGYDYGKGDF VEHACRYCGI HSPACVARCN VPSCRKWFCN
SRGNTSGSHL VNHLVRAKHK EVCLHKDSPL GETILECYNC GCRNVFLLGF ISAKAENVVV
LLCREPCLNV NALKDMNWDL SQWLPLIDDR CFLSWLVKVP SEQEQLRARQ ISAQQINKVE
ELWKTNPDAS LEDLEKPGVD DEPQPVVLKY EDAYQYQNVF APLIKLEADY DKMMKESQSK
DSVTVRWDIG LNKKRVAYFV FPKEDNELRL VPGDELRLRY SGGTSHPAWQ SVGHVIKLTA
QEEVALELRA SQGVPVELSH GFSVDFVWKS TSFDRMQGAM KTFAVDETSV SGYIYHHLLG
HEVEHQIIRN TLPKRFGAPG LPELNASQVL AVKSVLQKPV SLIQGPPGTG KTVTSAAIVY
HMAKQGQGQV LVCAPSNVAV DQLAEKISST GLKVVRLCAK SREAVSSPVE HLTLHYQVRH
LDTSEKSEMH KLQQLKDEQG ELSSSDEKKY KALKRATERE ILQSADVICC TCVGAGDPRL
SNFRFRQVLI DESTQATEPE CLIPLVLGVK QVVLVGDHCQ LGPVIMCKKA ARAGLAQSLF
ERLVILGVKP FRLQVQYRMH PCLSEFPSNC FYEGTLQNGV TVNERQSTGI DFPWPVPNRP
MFFYVQMGVE EISASGTSYL NRTEAANVEK IVTTFLRSGV VPSQIGVITP YEGQRAYIVN
YMSRNGSLRQ QLYKEIEVAS VDSFQGREKD YIILSCVRSN EHQGIGFLND PRRLNVALTR
ARYGIVVLGN PKVLSKQPLW NSLLTHYKEH ECLVEGPLNN LKQSMVQFQK PKKIYNDRRL
FLGGGQGVMH GSSFGTAGSV DKRSGRGKAH PFVPFGPPNG AHKPGVHPSY PLPRMPFPPF
PGSPHSQPYA IPTRGSLHGP IGAVPAVPQP GNRNFGAPRA NTGPIGGHLA AHQQNSQQAM
GNVGPAYNFT GLENPSSQPS GGGPMSQPGL MTQMPVQGLS QTFRDGFAIG GMSQDFFGDD
FKSQGSHVAY NIADFSTQAS QGGYGVEYTQ GPQSGYPGNY LNQSAHPGYP HMGATNEIVS
QDHMAHGSHG MFTQAGYNDP SQDESSQMHY GMGAPGHLQS QSMMNPMYSQ SYAHYNTQPQ
SLQPPPQ
//
