ID I1MC96_SOYBN Unreviewed; 507 AA.
AC I1MC96;
DT 13-JUN-2012, integrated into UniProtKB/TrEMBL.
DT 13-JUN-2012, sequence version 1.
DT 27-MAR-2024, entry version 63.
DE RecName: Full=Ent-kaurene oxidase {ECO:0008006|Google:ProtNLM};
GN Name=100814223 {ECO:0000313|EnsemblPlants:KRH09625};
GN ORFNames=GLYMA_15G002200 {ECO:0000313|EMBL:KRH09625.1};
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847 {ECO:0000313|EMBL:KRH09625.1};
RN [1] {ECO:0000313|EMBL:KRH09625.1, ECO:0000313|EnsemblPlants:KRH09625}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Williams 82 {ECO:0000313|EnsemblPlants:KRH09625};
RC TISSUE=Callus {ECO:0000313|EMBL:KRH09625.1};
RX PubMed=20075913; DOI=10.1038/nature08670;
RA Schmutz J., Cannon S.B., Schlueter J., Ma J., Mitros T., Nelson W.,
RA Hyten D.L., Song Q., Thelen J.J., Cheng J., Xu D., Hellsten U., May G.D.,
RA Yu Y., Sakurai T., Umezawa T., Bhattacharyya M.K., Sandhu D.,
RA Valliyodan B., Lindquist E., Peto M., Grant D., Shu S., Goodstein D.,
RA Barry K., Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N.,
RA Joshi T., Libault M., Sethuraman A., Zhang X.-C., Shinozaki K.,
RA Nguyen H.T., Wing R.A., Cregan P., Specht J., Grimwood J., Rokhsar D.,
RA Stacey G., Shoemaker R.C., Jackson S.A.;
RT "Genome sequence of the palaeopolyploid soybean.";
RL Nature 463:178-183(2010).
RN [2] {ECO:0000313|EnsemblPlants:KRH09625}
RP IDENTIFICATION.
RC STRAIN=Williams 82 {ECO:0000313|EnsemblPlants:KRH09625};
RG EnsemblPlants;
RL Submitted (FEB-2018) to UniProtKB.
RN [3] {ECO:0000313|EMBL:KRH09625.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Callus {ECO:0000313|EMBL:KRH09625.1};
RA Schmutz J., Cannon S., Schlueter J., Ma J., Mitros T., Nelson W., Hyten D.,
RA Song Q., Thelen J., Cheng J., Xu D., Hellsten U., May G., Yu Y.,
RA Sakurai T., Umezawa T., Bhattacharyya M., Sandhu D., Valliyodan B.,
RA Lindquist E., Peto M., Grant D., Shu S., Goodstein D., Barry K.,
RA Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N., Joshi T.,
RA Libault M., Sethuraman A., Zhang X., Shinozaki K., Nguyen H., Wing R.,
RA Cregan P., Specht J., Grimwood J., Rokhsar D., Stacey G., Shoemaker R.,
RA Jackson S.;
RT "WGS assembly of Glycine max.";
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family.
CC {ECO:0000256|RuleBase:RU000461}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CM000848; KRH09625.1; -; Genomic_DNA.
DR RefSeq; XP_003546599.1; XM_003546551.3.
DR AlphaFoldDB; I1MC96; -.
DR SMR; I1MC96; -.
DR STRING; 3847.I1MC96; -.
DR PaxDb; 3847-GLYMA15G00450-1; -.
DR EnsemblPlants; KRH09625; KRH09625; GLYMA_15G002200.
DR GeneID; 100814223; -.
DR Gramene; KRH09625; KRH09625; GLYMA_15G002200.
DR KEGG; gmx:100814223; -.
DR eggNOG; KOG0156; Eukaryota.
DR HOGENOM; CLU_001570_4_0_1; -.
DR InParanoid; I1MC96; -.
DR OMA; ACHMDER; -.
DR OrthoDB; 1584852at2759; -.
DR Proteomes; UP000008827; Chromosome 15.
DR GO; GO:0009707; C:chloroplast outer membrane; IBA:GO_Central.
DR GO; GO:0052615; F:ent-kaurene oxidase activity; IEA:InterPro.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0016709; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen; IBA:GO_Central.
DR GO; GO:0010241; P:ent-kaurene oxidation to kaurenoic acid; IBA:GO_Central.
DR GO; GO:0009686; P:gibberellin biosynthetic process; IBA:GO_Central.
DR CDD; cd11075; CYP77_89; 1.
DR Gene3D; 1.10.630.10; Cytochrome P450; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR InterPro; IPR044225; KO_chloroplastic.
DR PANTHER; PTHR47283; ENT-KAURENE OXIDASE, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47283:SF1; ENT-KAURENE OXIDASE, CHLOROPLASTIC; 1.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; Cytochrome P450; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 3: Inferred from homology;
KW Heme {ECO:0000256|RuleBase:RU000461}; Iron {ECO:0000256|RuleBase:RU000461};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Metal-binding {ECO:0000256|RuleBase:RU000461};
KW Monooxygenase {ECO:0000256|RuleBase:RU000461};
KW Oxidoreductase {ECO:0000256|RuleBase:RU000461};
KW Reference proteome {ECO:0000313|Proteomes:UP000008827};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989}.
SQ SEQUENCE 507 AA; 57554 MW; 528C9105DD60ADB2 CRC64;
MESLTLSVTV AVVAFSILLF LLLLTRRHAG VGAGAGAGSL PPVPAVPGLP VIGNLLQLKE
KKPYKTFTHM THKHGPIYSI RTGASTLIVL NSPHLAKEAM VTRFSSISTR KLSNALKILS
SDKCMVATSD YNEFHKTVKR HILTNLSGAN AQKRHRIRRE AMMENILSQF SEHIKTFSDL
AANFRKIFAT QLFGLALKQA LGSNVETIYV EELGSTLSKE DIYKILVVDI SEGAIEVDWR
DFFPYLKWIP NRRMEMKIQN LHVRRKAVMK ALMNEQKNRM ASGKKVHCYF DYLVSEAKEL
TEDQISMLIW ETIIGTSDTT LVTTEWAMYE LAKDKTRQDR LYEELQYVCG HENVIEDQLS
KLPYLGAVFH ETLRKHSPAP MVPPRYVHED TQLGGYHIPA GSEIAINIYG CNMDSNRWEN
PYEWMPERFL DEKYDPVDLF KTMAFGAGKR VCAGSLQAML IACTAIGRLV QEFEWELGQG
EEENVNTQCF TTRKLHPLLV KLKPRIK
//
