ID I1N3A5_SOYBN Unreviewed; 462 AA.
AC I1N3A5;
DT 13-JUN-2012, integrated into UniProtKB/TrEMBL.
DT 09-JAN-2013, sequence version 2.
DT 27-MAR-2024, entry version 81.
DE RecName: Full=mannan endo-1,4-beta-mannosidase {ECO:0000256|ARBA:ARBA00012706};
DE EC=3.2.1.78 {ECO:0000256|ARBA:ARBA00012706};
GN Name=100811794 {ECO:0000313|EnsemblPlants:KRH00473};
GN ORFNames=GLYMA_18G215600 {ECO:0000313|EMBL:KRH00473.1};
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847 {ECO:0000313|EMBL:KRH00473.1};
RN [1] {ECO:0000313|EMBL:KRH00473.1, ECO:0000313|EnsemblPlants:KRH00473}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Williams 82 {ECO:0000313|EnsemblPlants:KRH00473};
RC TISSUE=Callus {ECO:0000313|EMBL:KRH00473.1};
RX PubMed=20075913; DOI=10.1038/nature08670;
RA Schmutz J., Cannon S.B., Schlueter J., Ma J., Mitros T., Nelson W.,
RA Hyten D.L., Song Q., Thelen J.J., Cheng J., Xu D., Hellsten U., May G.D.,
RA Yu Y., Sakurai T., Umezawa T., Bhattacharyya M.K., Sandhu D.,
RA Valliyodan B., Lindquist E., Peto M., Grant D., Shu S., Goodstein D.,
RA Barry K., Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N.,
RA Joshi T., Libault M., Sethuraman A., Zhang X.-C., Shinozaki K.,
RA Nguyen H.T., Wing R.A., Cregan P., Specht J., Grimwood J., Rokhsar D.,
RA Stacey G., Shoemaker R.C., Jackson S.A.;
RT "Genome sequence of the palaeopolyploid soybean.";
RL Nature 463:178-183(2010).
RN [2] {ECO:0000313|EnsemblPlants:KRH00473}
RP IDENTIFICATION.
RC STRAIN=Williams 82 {ECO:0000313|EnsemblPlants:KRH00473};
RG EnsemblPlants;
RL Submitted (FEB-2018) to UniProtKB.
RN [3] {ECO:0000313|EMBL:KRH00473.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Callus {ECO:0000313|EMBL:KRH00473.1};
RA Schmutz J., Cannon S., Schlueter J., Ma J., Mitros T., Nelson W., Hyten D.,
RA Song Q., Thelen J., Cheng J., Xu D., Hellsten U., May G., Yu Y.,
RA Sakurai T., Umezawa T., Bhattacharyya M., Sandhu D., Valliyodan B.,
RA Lindquist E., Peto M., Grant D., Shu S., Goodstein D., Barry K.,
RA Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N., Joshi T.,
RA Libault M., Sethuraman A., Zhang X., Shinozaki K., Nguyen H., Wing R.,
RA Cregan P., Specht J., Grimwood J., Rokhsar D., Stacey G., Shoemaker R.,
RA Jackson S.;
RT "WGS assembly of Glycine max.";
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in
CC mannans, galactomannans and glucomannans.; EC=3.2.1.78;
CC Evidence={ECO:0000256|ARBA:ARBA00001678};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC {ECO:0000256|ARBA:ARBA00005641, ECO:0000256|RuleBase:RU361153}.
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DR EMBL; CM000851; KRH00473.1; -; Genomic_DNA.
DR RefSeq; XP_003552321.1; XM_003552273.3.
DR AlphaFoldDB; I1N3A5; -.
DR SMR; I1N3A5; -.
DR STRING; 3847.I1N3A5; -.
DR PaxDb; 3847-GLYMA18G44810-1; -.
DR EnsemblPlants; KRH00473; KRH00473; GLYMA_18G215600.
DR GeneID; 100811794; -.
DR Gramene; KRH00473; KRH00473; GLYMA_18G215600.
DR KEGG; gmx:100811794; -.
DR eggNOG; ENOG502QU0Z; Eukaryota.
DR HOGENOM; CLU_031603_0_0_1; -.
DR InParanoid; I1N3A5; -.
DR OMA; ISNYGEM; -.
DR OrthoDB; 2717493at2759; -.
DR Proteomes; UP000008827; Chromosome 18.
DR ExpressionAtlas; I1N3A5; baseline and differential.
DR GO; GO:0016985; F:mannan endo-1,4-beta-mannosidase activity; IBA:GO_Central.
DR GO; GO:0071704; P:organic substance metabolic process; IEA:InterPro.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR001547; Glyco_hydro_5.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR045053; MAN-like.
DR PANTHER; PTHR31451:SF54; MANNAN ENDO-1,4-BETA-MANNOSIDASE 6; 1.
DR PANTHER; PTHR31451; MANNAN ENDO-1,4-BETA-MANNOSIDASE 7; 1.
DR Pfam; PF00150; Cellulase; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361153};
KW Hydrolase {ECO:0000256|RuleBase:RU361153};
KW Reference proteome {ECO:0000313|Proteomes:UP000008827};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..23
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 24..462
FT /note="mannan endo-1,4-beta-mannosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5014579280"
FT DOMAIN 75..375
FT /note="Glycoside hydrolase family 5"
FT /evidence="ECO:0000259|Pfam:PF00150"
SQ SEQUENCE 462 AA; 52490 MW; B95A68F124858E1F CRC64;
MERFGVRIIS LILLLMLTKN LSSNALGDSM KDEEGLKEDQ NEMENSLSNY DEMRGMKDDD
WQMVQKKGNH FVVNDKPFYV NGFNTYWLMV FAADESTRGK VTEVFKQASS VGMTVCRTWA
FNDGQWRALQ KSPSVYDEEV FKALDFVVSE AKKYKIRLIL SLANNWEAYG GKAQYVKWGN
AAGLNLTSDD DFFSHPTLRS YYKAHVKTVL NRVNTFTNIT YKEDPTIFAW ELMNEPRCTS
DPTGDKLQDW IQEMAFHVKK IDPKHLVEVG VEGFYGPSTP QRTQFNPNTY ATQVGTDFIR
NHLVLGVDFA SVHIYADSWI SQQIADTHIP FIKSWMEAHI EDAEKYLGMP VVFAEFGVSS
KDPGYNSSYR DTVISTVYST ILNSTKKGGS GAGSLLWQFF PDGTDNMDDG YAIVLSKSPS
TSGIIQLQST RLALFNSLCN TKCNWGCKKK KLLEEILYRD EL
//
