ID I1XKQ1_METNJ Unreviewed; 531 AA.
AC I1XKQ1;
DT 11-JUL-2012, integrated into UniProtKB/TrEMBL.
DT 11-JUL-2012, sequence version 1.
DT 27-MAR-2024, entry version 53.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN OrderedLocusNames=Q7A_2156 {ECO:0000313|EMBL:AFI84970.1};
OS Methylophaga nitratireducenticrescens.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Thiotrichales;
OC Piscirickettsiaceae; Methylophaga.
OX NCBI_TaxID=754476 {ECO:0000313|EMBL:AFI84970.1, ECO:0000313|Proteomes:UP000009144};
RN [1] {ECO:0000313|EMBL:AFI84970.1, ECO:0000313|Proteomes:UP000009144}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JAM1 {ECO:0000313|EMBL:AFI84970.1,
RC ECO:0000313|Proteomes:UP000009144};
RX PubMed=22815445; DOI=10.1128/JB.00726-12;
RA Villeneuve C., Martineau C., Mauffrey F., Villemur R.;
RT "Complete genome sequences of Methylophaga sp. strain JAM1 and Methylophaga
RT sp. strain JAM7.";
RL J. Bacteriol. 194:4126-4127(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; CP003390; AFI84970.1; -; Genomic_DNA.
DR RefSeq; WP_014707338.1; NC_017857.3.
DR AlphaFoldDB; I1XKQ1; -.
DR STRING; 754476.Q7A_2156; -.
DR REBASE; 48173; M.MspJAM1ORF2154P.
DR KEGG; mej:Q7A_2156; -.
DR PATRIC; fig|754476.3.peg.2133; -.
DR eggNOG; COG0286; Bacteria.
DR HOGENOM; CLU_013049_0_2_6; -.
DR OrthoDB; 5749002at2; -.
DR Proteomes; UP000009144; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR004546; Restrct_endonuc_T1M.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR NCBIfam; TIGR00497; hsdM; 1.
DR PANTHER; PTHR42933:SF1; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); 1.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:AFI84970.1};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:AFI84970.1}.
FT DOMAIN 9..177
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 189..493
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 531 AA; 60025 MW; 1D6AD62E4BCC7570 CRC64;
MTEDQKKKLE QKLWNIANEL RGKMNADEFR DYCLGFIFYK YLSEKLHTFA NGIMAEDGID
FMAIDENSPQ GQEMLEAISE ESIEELGYFL KPSELFTSLA KRGAHKVEDA NADDDVVVDN
FILDDLSKVL NSIERSTMGT ESEDDFDHLF EDLDLTSTKL GRTEDAKNKL IAKILVHLDN
IDFAISDSES DVLGDAYEYL IGQFAAGAGK KAGEFYTPQA VSTILAKLVT NGKTRLKSVY
DPTCGSGSLL LRVSKEVEHV GDFYGQEMNR TTFNLARMNM ILHGVHYRNF DLKQEDTLEH
PQHIDKKFEA VVANPPFSAK WSASKLFESD DRFARYGKLA PGSKADFAFV QHMLYQLEEN
GIMAVVLPHG VLFRGAAEGH IRRYIIEEGN WLDAVIGLPA NIFYGTSIPT CILVLKKCRE
HPDNILFIDA SNHFEKAKNQ NYLRPEHIEK IITAYRERKD EAKYSHVASL SEISENDYNL
NIPRYVDTFE EAEPVDLAAV TAELKKLDSE MVALDEKIQS FCTELGIETP V
//
