ID I1YB50_ECOLX Unreviewed; 750 AA.
AC I1YB50;
DT 11-JUL-2012, integrated into UniProtKB/TrEMBL.
DT 11-JUL-2012, sequence version 1.
DT 24-JAN-2024, entry version 47.
DE SubName: Full=Conjugal transfer coupling protein TraD {ECO:0000313|EMBL:SPE00251.1};
DE SubName: Full=Conjugal transfer protein TraD {ECO:0000313|EMBL:AFI99259.1};
GN Name=traD {ECO:0000313|EMBL:AFI99259.1};
GN ORFNames=RCS57TR364_P0032 {ECO:0000313|EMBL:SPE00251.1}, RCS65_P0050
GN {ECO:0000313|EMBL:SPE01583.1};
OS Escherichia coli.
OG Plasmid RCS57TR364_p {ECO:0000313|EMBL:SPE00251.1},
OG Plasmid RCS65_p {ECO:0000313|EMBL:SPE01583.1},
OG Plasmid pCA08 {ECO:0000313|EMBL:AKD26831.1},
OG Plasmid pFOS-HK151325 {ECO:0000313|EMBL:AFV92849.1}, and
OG Plasmid pHK23a {ECO:0000313|EMBL:AFI99259.1}.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562 {ECO:0000313|EMBL:AFI99259.1};
RN [1] {ECO:0000313|EMBL:AFV92849.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ECBC1G4 {ECO:0000313|EMBL:AFV92849.1};
RC PLASMID=pFOS-HK151325 {ECO:0000313|EMBL:AFV92849.1};
RX PubMed=23988630; DOI=10.1099/jmm.0.062653-0;
RA Ho P.L., Chan J., Lo W.U., Lai E.L., Cheung Y.Y., Lau T.C., Chow K.H.;
RT "Prevalence and molecular epidemiology of plasmid-mediated fosfomycin
RT resistance genes among blood and urinary Escherichia coli isolates.";
RL J. Med. Microbiol. 62:1707-1713(2013).
RN [2] {ECO:0000313|EMBL:AFI99259.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=P0022_ST {ECO:0000313|EMBL:AFI99259.1};
RC PLASMID=pHK23a {ECO:0000313|EMBL:AFI99259.1};
RX PubMed=23078751; DOI=10.1016/j.vetmic.2012.09.023;
RA Ho P.L., Chan J., Lo W.U., Law P.Y., Chow K.H.;
RT "Plasmid-mediated fosfomycin resistance in Escherichia coli isolated from
RT pig.";
RL Vet. Microbiol. 162:964-967(2013).
RN [3] {ECO:0000313|EMBL:AKD26831.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CA08 {ECO:0000313|EMBL:AKD26831.1};
RC PLASMID=pCA08 {ECO:0000313|EMBL:AKD26831.1};
RX PubMed=25753630; DOI=10.1128/AAC.04772-14;
RA Li J.J., Spychala C.N., Hu F., Sheng J.F., Doi Y.;
RT "Complete nucleotide sequences of bla(CTX-M)-harboring IncF plasmids from
RT community-associated Escherichia coli strains in the United States.";
RL Antimicrob. Agents Chemother. 59:3002-3007(2015).
RN [4] {ECO:0000313|EMBL:AKD26831.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CA08 {ECO:0000313|EMBL:AKD26831.1};
RC PLASMID=pCA08 {ECO:0000313|EMBL:AKD26831.1};
RA Li J.-J., Doi Y.;
RT "CTX-M-encoding plasmid pCA08 from community associated E. coli.";
RL Submitted (APR-2015) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|EMBL:SPE00251.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=364 {ECO:0000313|EMBL:SPE00251.1}, and 522
RC {ECO:0000313|EMBL:SPE01583.1};
RC PLASMID=RCS57TR364_p {ECO:0000313|EMBL:SPE00251.1}, and RCS65_p
RC {ECO:0000313|EMBL:SPE01583.1};
RA Cohen D.B., Kent A.D.;
RL Submitted (FEB-2018) to the EMBL/GenBank/DDBJ databases.
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DR EMBL; JQ432559; AFI99259.1; -; Genomic_DNA.
DR EMBL; JX627737; AFV92849.1; -; Genomic_DNA.
DR EMBL; CP009233; AKD26831.1; -; Genomic_DNA.
DR EMBL; LT985265; SPE00251.1; -; Genomic_DNA.
DR EMBL; LT985277; SPE01583.1; -; Genomic_DNA.
DR RefSeq; WP_015059147.1; NZ_SSII01000064.1.
DR RefSeq; YP_006953979.1; NC_019090.1.
DR RefSeq; YP_006990791.1; NC_019424.1.
DR AlphaFoldDB; I1YB50; -.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR CDD; cd01127; TrwB_TraG_TraD_VirD4; 1.
DR Gene3D; 1.10.8.80; Magnesium chelatase subunit I, C-Terminal domain; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014128; T4SS_TraD.
DR InterPro; IPR019476; T4SS_TraD_DNA-bd.
DR InterPro; IPR022585; TraD_N.
DR NCBIfam; TIGR02759; TraD_Ftype; 1.
DR PANTHER; PTHR37937; CONJUGATIVE TRANSFER: DNA TRANSPORT; 1.
DR PANTHER; PTHR37937:SF1; CONJUGATIVE TRANSFER: DNA TRANSPORT; 1.
DR Pfam; PF12615; TraD_N; 1.
DR Pfam; PF10412; TrwB_AAD_bind; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 4: Predicted;
KW Membrane {ECO:0000256|SAM:Phobius}; Plasmid {ECO:0000313|EMBL:AFI99259.1};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 28..47
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 108..134
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 32..128
FT /note="TraD coupling protein N-terminal"
FT /evidence="ECO:0000259|Pfam:PF12615"
FT DOMAIN 172..561
FT /note="Type IV secretion system coupling protein TraD DNA-
FT binding"
FT /evidence="ECO:0000259|Pfam:PF10412"
FT REGION 607..702
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 624..653
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 750 AA; 85314 MW; D394795748121C48 CRC64;
MSFNAKDMTQ GGQIASMRIR MFSQIANIML YCLFIFFWIL IGLVLWVKIS WQTFINGCIY
WWCTSLEGMR DLIKSQPVYE IQYYGKTFRM NAAQVLHDKY MIWCGEQLWS AFVLASVVAL
VICLITFFVV SWILGRQGKQ QSENEVTGGR QLTDNPKDVA RMLKKDGKDS DIRIGDLPII
RDSEIQNFCL HGTVGAGKSE VIRRLANYAR QRGDMVVIYD RSGEFVKSYY DPSIDKILNP
LDARCAAWDL WKECLTQPDF DNTANTLIPM GTKEDPFWQG SGRTIFAEAA YLMRNDPNRS
YSKLVDTLLS IKIEKLRTFL RNSPAANLVE EKIEKTAISI RAVLTNYVKA IRYLQGIEHN
GDPFTIRDWM RGVREDQKNG WLFISSNADT HASLKPVISM WLSIAIRGLL AMGENRNRRV
WFFCDELPTL HKLPDLVEIL PEARKFGGCY VFGIQSYAQL EDIYGEKAAA TLFDVMNTRA
FFRSPSHKIA EFAAGEIGEK EHLKASEQYS YGADPVRDGV STGKDMERQT LVSYSDIQSL
PDLTCYVTLP GPYPAVKLSL KYQARPKVAP EFIPRDINPE MENRLSAVLA AREAEGRQMA
SLFEPEVASG EDVTQAEQPQ QPQQPQQPQQ PQQPQQPQQP QQPQQPQQPQ QPQQPQQPVS
SVINDKKSDA GVSVPAGGIE QELKMKPEEE MEQQLPPGIS ESGEVVDMAA YEAWQQENHP
DIQQHMQRRE EVNINVHRER GEDVEPGDDF
//
