ID   I3IVM8_ORENI            Unreviewed;       447 AA.
AC   I3IVM8;
DT   11-JUL-2012, integrated into UniProtKB/TrEMBL.
DT   17-JUN-2020, sequence version 2.
DT   27-MAR-2024, entry version 60.
DE   RecName: Full=cysteine desulfurase {ECO:0000256|ARBA:ARBA00012239};
DE            EC=2.8.1.7 {ECO:0000256|ARBA:ARBA00012239};
GN   Name=nfs1 {ECO:0000313|Ensembl:ENSONIP00000000668.2};
OS   Oreochromis niloticus (Nile tilapia) (Tilapia nilotica).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Ovalentaria; Cichlomorphae; Cichliformes; Cichlidae; African cichlids;
OC   Pseudocrenilabrinae; Oreochromini; Oreochromis.
OX   NCBI_TaxID=8128 {ECO:0000313|Ensembl:ENSONIP00000000668.2, ECO:0000313|Proteomes:UP000005207};
RN   [1] {ECO:0000313|Proteomes:UP000005207}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG   Broad Institute Genome Assembly Team;
RG   Broad Institute Sequencing Platform;
RA   Di Palma F., Johnson J., Lander E.S., Lindblad-Toh K.;
RT   "The Genome Sequence of Oreochromis niloticus (Nile Tilapia).";
RL   Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|Ensembl:ENSONIP00000000668.2}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000256|ARBA:ARBA00001933,
CC         ECO:0000256|RuleBase:RU004504};
CC   -!- SIMILARITY: Belongs to the class-V pyridoxal-phosphate-dependent
CC       aminotransferase family. NifS/IscS subfamily.
CC       {ECO:0000256|ARBA:ARBA00006490}.
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DR   RefSeq; XP_003447759.1; XM_003447711.4.
DR   AlphaFoldDB; I3IVM8; -.
DR   STRING; 8128.ENSONIP00000000668; -.
DR   Ensembl; ENSONIT00000000667.2; ENSONIP00000000668.2; ENSONIG00000000523.2.
DR   GeneID; 100697694; -.
DR   KEGG; onl:100697694; -.
DR   CTD; 9054; -.
DR   eggNOG; KOG1549; Eukaryota.
DR   GeneTree; ENSGT00940000155740; -.
DR   HOGENOM; CLU_003433_0_2_1; -.
DR   InParanoid; I3IVM8; -.
DR   OMA; KGLYWAR; -.
DR   OrthoDB; 212394at2759; -.
DR   TreeFam; TF105658; -.
DR   Proteomes; UP000005207; Linkage group LG5.
DR   GO; GO:0031071; F:cysteine desulfurase activity; IEA:UniProtKB-EC.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR   GO; GO:0044571; P:[2Fe-2S] cluster assembly; IEA:InterPro.
DR   Gene3D; 3.90.1150.10; Aspartate Aminotransferase, domain 1; 1.
DR   Gene3D; 3.40.640.10; Type I PLP-dependent aspartate aminotransferase-like (Major domain); 1.
DR   HAMAP; MF_00331; Cys_desulf_IscS; 1.
DR   InterPro; IPR000192; Aminotrans_V_dom.
DR   InterPro; IPR020578; Aminotrans_V_PyrdxlP_BS.
DR   InterPro; IPR010240; Cys_deSase_IscS.
DR   InterPro; IPR016454; Cysteine_dSase.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR   NCBIfam; TIGR02006; IscS; 1.
DR   PANTHER; PTHR11601:SF34; CYSTEINE DESULFURASE, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR11601; CYSTEINE DESULFURYLASE FAMILY MEMBER; 1.
DR   Pfam; PF00266; Aminotran_5; 1.
DR   PIRSF; PIRSF005572; NifS; 1.
DR   SUPFAM; SSF53383; PLP-dependent transferases; 1.
DR   PROSITE; PS00595; AA_TRANSFER_CLASS_5; 1.
PE   3: Inferred from homology;
KW   Iron {ECO:0000256|ARBA:ARBA00023004};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Pyridoxal phosphate {ECO:0000256|ARBA:ARBA00022898};
KW   Reference proteome {ECO:0000313|Proteomes:UP000005207}.
FT   DOMAIN          50..411
FT                   /note="Aminotransferase class V"
FT                   /evidence="ECO:0000259|Pfam:PF00266"
SQ   SEQUENCE   447 AA;  49745 MW;  A26AE9B5B89B2A3E CRC64;
     MLASGRTVSS RLLRPTAWLP LRLSSERTAA SSPQKELIKK HEQDELRPLY MDFQATTPMD
     PRVLDAMLPY QVNYYGNPHS RTHAYGWESE SAMETARKQV ADLIGADPRE IVFTSGATES
     NNMAIKGVAR FYKAKKRHVI TTQTEHKCVL DSCRILESEG FDVTYLPVQK NGLLDLELLE
     ASIRTDTSLV SVMTVNNEIG VKQPIKEIGQ ICRSKGVFFH TDAAQAVGKI PISISDWKID
     LMSISGHKIY GPKGVGALYV RRRPRVRLEP IQNGGGQERG LRSGTVPTPL AVGLGAACSV
     AQKEMEYDHQ RVSMLANRLV QKIMSEIPDV IMNGDPEQRY PGCINLSFAY VEGESLLMAL
     KDVALSSGSA CTSASLEPSY VLRAIGADED LAHSSIRFGI GRFTTEEEVD YTAEKCIEQV
     RRLREMSPLW EMIQEGIDLK SIKWTQH
//