ID I3J3V2_ORENI Unreviewed; 606 AA.
AC I3J3V2;
DT 11-JUL-2012, integrated into UniProtKB/TrEMBL.
DT 17-JUN-2020, sequence version 2.
DT 27-MAR-2024, entry version 65.
DE RecName: Full=78 kDa glucose-regulated protein {ECO:0000256|ARBA:ARBA00029695};
DE AltName: Full=Binding-immunoglobulin protein {ECO:0000256|ARBA:ARBA00031291};
DE AltName: Full=Heat shock protein 70 family protein 5 {ECO:0000256|ARBA:ARBA00031035};
DE AltName: Full=Heat shock protein family A member 5 {ECO:0000256|ARBA:ARBA00030223};
DE AltName: Full=Immunoglobulin heavy chain-binding protein {ECO:0000256|ARBA:ARBA00033037};
GN Name=LOC100708428 {ECO:0000313|Ensembl:ENSONIP00000003542.2};
OS Oreochromis niloticus (Nile tilapia) (Tilapia nilotica).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Cichlomorphae; Cichliformes; Cichlidae; African cichlids;
OC Pseudocrenilabrinae; Oreochromini; Oreochromis.
OX NCBI_TaxID=8128 {ECO:0000313|Ensembl:ENSONIP00000003542.2, ECO:0000313|Proteomes:UP000005207};
RN [1] {ECO:0000313|Proteomes:UP000005207}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG Broad Institute Genome Assembly Team;
RG Broad Institute Sequencing Platform;
RA Di Palma F., Johnson J., Lander E.S., Lindblad-Toh K.;
RT "The Genome Sequence of Oreochromis niloticus (Nile Tilapia).";
RL Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSONIP00000003542.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum lumen
CC {ECO:0000256|ARBA:ARBA00004319}.
CC -!- SIMILARITY: Belongs to the heat shock protein 70 family.
CC {ECO:0000256|ARBA:ARBA00007381, ECO:0000256|RuleBase:RU003322}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR AlphaFoldDB; I3J3V2; -.
DR STRING; 8128.ENSONIP00000031363; -.
DR Ensembl; ENSONIT00000003543.2; ENSONIP00000003542.2; ENSONIG00000039112.1.
DR eggNOG; KOG0100; Eukaryota.
DR GeneTree; ENSGT00940000154787; -.
DR HOGENOM; CLU_005965_0_0_1; -.
DR TreeFam; TF105044; -.
DR Proteomes; UP000005207; Linkage group LG7.
DR GO; GO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR CDD; cd10241; HSPA5-like_NBD; 1.
DR Gene3D; 3.30.420.40; -; 2.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR042050; BIP_NBD.
DR InterPro; IPR018181; Heat_shock_70_CS.
DR InterPro; IPR029047; HSP70_peptide-bd_sf.
DR InterPro; IPR013126; Hsp_70_fam.
DR PANTHER; PTHR19375:SF144; ENDOPLASMIC RETICULUM CHAPERONE BIP; 1.
DR PANTHER; PTHR19375; HEAT SHOCK PROTEIN 70KDA; 1.
DR Pfam; PF00012; HSP70; 1.
DR PRINTS; PR00301; HEATSHOCK70.
DR SUPFAM; SSF53067; Actin-like ATPase domain; 2.
DR SUPFAM; SSF100920; Heat shock protein 70kD (HSP70), peptide-binding domain; 1.
DR PROSITE; PS00297; HSP70_1; 1.
DR PROSITE; PS00329; HSP70_2; 1.
DR PROSITE; PS01036; HSP70_3; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU003322};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU003322};
KW Reference proteome {ECO:0000313|Proteomes:UP000005207};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..16
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 17..606
FT /note="78 kDa glucose-regulated protein"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5025545884"
FT TRANSMEM 581..603
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT COILED 277..304
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 606 AA; 66868 MW; B8E44CFC19B7164A CRC64;
MKLFWVVMLV VGTVFAADDE DKKDNVGTVV GIDLGTTYSC VGVFKNGRVE IIANDQGNRI
TPSYVAFTSE GERLIGDAAK NQLTSNPENT IFDAKRLIGR TWGDPSVQQD IKYLPFKVTE
KKSKPHIQVD IGGGQMKTFA PEEVSAMVLT KMKETAEAYL GKKVTHAVVT VPAYFNDAQR
QATKDAGTIA GLNVMRIINE PTAAAIAYGL DKKDGEKNIL VFDLGGGTFD VSLLTIDNGV
FEVVATNGDT HLGGEDFDQR VMEHFIKLYK KKTGKDVRKD NRAVQKLRRE VEKAKRALSA
QHQARIEIES FFEGEDFSET LTRAKFEELN MDLFRSTMKP VQKVLEDSDL KKSDIDEIVL
VGGSTRIPKI QQLVKEFFNG KEPSRGINPD EAVAYGAAVQ AGVLSGEEDT GDVVLLDVCP
LTLGIETVGG VMTKLIPRNT VVPTKKAQIF STASDNQPTV TIKVYEGERP LTKDNHLLGT
FDLTGIPPAP RGVPQIEVTF EIDVNGILRV TAEDKGTGNK NKITITNDQN RLTPEDIERM
VNDAERFADE DKKLKERIDA RNELESYAYS LKNQIGDKEK LGGLFMFTGS AKVYLLNLAI
VLLERH
//
