ID   I3J660_ORENI            Unreviewed;       569 AA.
AC   I3J660;
DT   11-JUL-2012, integrated into UniProtKB/TrEMBL.
DT   17-JUN-2020, sequence version 2.
DT   27-MAR-2024, entry version 67.
DE   RecName: Full=Eukaryotic peptide chain release factor GTP-binding subunit {ECO:0000256|ARBA:ARBA00015765};
DE   AltName: Full=ERF2 {ECO:0000256|ARBA:ARBA00030845};
DE   AltName: Full=Polypeptide release factor 3 {ECO:0000256|ARBA:ARBA00029585};
DE   AltName: Full=Translation release factor 3 {ECO:0000256|ARBA:ARBA00030210};
GN   Name=LOC100701961 {ECO:0000313|Ensembl:ENSONIP00000004350.2};
OS   Oreochromis niloticus (Nile tilapia) (Tilapia nilotica).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Ovalentaria; Cichlomorphae; Cichliformes; Cichlidae; African cichlids;
OC   Pseudocrenilabrinae; Oreochromini; Oreochromis.
OX   NCBI_TaxID=8128 {ECO:0000313|Ensembl:ENSONIP00000004350.2, ECO:0000313|Proteomes:UP000005207};
RN   [1] {ECO:0000313|Proteomes:UP000005207}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG   Broad Institute Genome Assembly Team;
RG   Broad Institute Sequencing Platform;
RA   Di Palma F., Johnson J., Lander E.S., Lindblad-Toh K.;
RT   "The Genome Sequence of Oreochromis niloticus (Nile Tilapia).";
RL   Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|Ensembl:ENSONIP00000004350.2}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A
CC       subfamily. {ECO:0000256|ARBA:ARBA00007249}.
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DR   AlphaFoldDB; I3J660; -.
DR   STRING; 8128.ENSONIP00000046715; -.
DR   Ensembl; ENSONIT00000004352.2; ENSONIP00000004350.2; ENSONIG00000003456.2.
DR   eggNOG; KOG0459; Eukaryota.
DR   GeneTree; ENSGT00940000155582; -.
DR   HOGENOM; CLU_007265_3_8_1; -.
DR   TreeFam; TF300566; -.
DR   Proteomes; UP000005207; Linkage group LG4.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   CDD; cd01883; EF1_alpha; 1.
DR   CDD; cd03704; eRF3_C_III; 1.
DR   CDD; cd04089; eRF3_II; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   Gene3D; 2.40.30.10; Translation factors; 2.
DR   InterPro; IPR004161; EFTu-like_2.
DR   InterPro; IPR031157; G_TR_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR   InterPro; IPR009000; Transl_B-barrel_sf.
DR   InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR   InterPro; IPR004160; Transl_elong_EFTu/EF1A_C.
DR   PANTHER; PTHR23115:SF36; TR-TYPE G DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR23115; TRANSLATION FACTOR; 1.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF03144; GTP_EFTU_D2; 1.
DR   Pfam; PF03143; GTP_EFTU_D3; 1.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SUPFAM; SSF50465; EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   SUPFAM; SSF50447; Translation proteins; 1.
DR   PROSITE; PS00301; G_TR_1; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
PE   3: Inferred from homology;
KW   GTP-binding {ECO:0000256|ARBA:ARBA00023134};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Reference proteome {ECO:0000313|Proteomes:UP000005207}.
FT   DOMAIN          147..373
FT                   /note="Tr-type G"
FT                   /evidence="ECO:0000259|PROSITE:PS51722"
FT   REGION          57..125
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        89..103
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   569 AA;  62974 MW;  CC409E627A4DF6B6 CRC64;
     MDPRDTAPDS WEQEDDVEAT IDGQLESAFT TLNVNAKPFV PNVNAAEFVP AFGLKAHPGN
     VDSAGEYRGT TGESEAGSPV ENGDAEMTTE EPWDQKEVEP SEEEPGGGVC VDRGPEGPDE
     TAPEMMEEEE EVLVPKVPPT QPDAPKKEHI NVVFIGHVDA GKSTIGGQIM YLTGMVDKRT
     LEKYEREAKE KNRETWYLSW ALDTNQEERD KGKTVEVGRA YFETDKKHFT ILDAPGHKSF
     VPNMIGGASQ ADLAVLVISA RKGEFETGFE KGGQTREHAM LAKTAGVKHL IVLVNKMDDP
     TVNWSLERYE ECKEKLVPFL KKVGFNPKKD IHFMPCSGLT GANLKEPTNM CSWYTGLPFI
     PHLDSLPNFN RSSDGPVRLP IVDKYKDMGT VILGKLESGS ISKAQQLVMM PNRHVVEVLS
     LLSDDVETDD AGPGENLKLR LKGIEEEEIL PGFILCNAEN LCHSGRTFDA QIVIIEHKSI
     ICPGYNAVLH IHTCIEEVQI TALICLVDKK TGEKSKTRPR FVKQDQVCIA RLRTAGTICL
     ETFKDFPQMG RFTLRDEGVC AYISIILSV
//