ID I3K5G4_ORENI Unreviewed; 685 AA.
AC I3K5G4;
DT 11-JUL-2012, integrated into UniProtKB/TrEMBL.
DT 17-JUN-2020, sequence version 2.
DT 27-MAR-2024, entry version 55.
DE RecName: Full=glutamine--fructose-6-phosphate transaminase (isomerizing) {ECO:0000256|ARBA:ARBA00012916};
DE EC=2.6.1.16 {ECO:0000256|ARBA:ARBA00012916};
GN Name=LOC100692652 {ECO:0000313|Ensembl:ENSONIP00000016359.2};
OS Oreochromis niloticus (Nile tilapia) (Tilapia nilotica).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Cichlomorphae; Cichliformes; Cichlidae; African cichlids;
OC Pseudocrenilabrinae; Oreochromini; Oreochromis.
OX NCBI_TaxID=8128 {ECO:0000313|Ensembl:ENSONIP00000016359.2, ECO:0000313|Proteomes:UP000005207};
RN [1] {ECO:0000313|Proteomes:UP000005207}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG Broad Institute Genome Assembly Team;
RG Broad Institute Sequencing Platform;
RA Di Palma F., Johnson J., Lander E.S., Lindblad-Toh K.;
RT "The Genome Sequence of Oreochromis niloticus (Nile Tilapia).";
RL Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSONIP00000016359.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-
CC phosphate + L-glutamate; Xref=Rhea:RHEA:13237, ChEBI:CHEBI:29985,
CC ChEBI:CHEBI:58359, ChEBI:CHEBI:58725, ChEBI:CHEBI:61527; EC=2.6.1.16;
CC Evidence={ECO:0000256|ARBA:ARBA00001031};
CC -!- PATHWAY: Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-
CC glucosamine biosynthesis; alpha-D-glucosamine 6-phosphate from D-
CC fructose 6-phosphate: step 1/1. {ECO:0000256|ARBA:ARBA00004775}.
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DR RefSeq; XP_019222572.1; XM_019367027.1.
DR AlphaFoldDB; I3K5G4; -.
DR STRING; 8128.ENSONIP00000081467; -.
DR Ensembl; ENSONIT00000016374.2; ENSONIP00000016359.2; ENSONIG00000013006.2.
DR GeneID; 100692652; -.
DR KEGG; onl:100692652; -.
DR CTD; 9945; -.
DR eggNOG; KOG1268; Eukaryota.
DR GeneTree; ENSGT00940000156413; -.
DR HOGENOM; CLU_012520_5_2_1; -.
DR OrthoDB; 1705390at2759; -.
DR TreeFam; TF300864; -.
DR UniPathway; UPA00113; UER00528.
DR Proteomes; UP000005207; Linkage group LG2.
DR GO; GO:0097367; F:carbohydrate derivative binding; IEA:InterPro.
DR GO; GO:0004360; F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity; IEA:UniProtKB-EC.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0006048; P:UDP-N-acetylglucosamine biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00714; GFAT; 1.
DR CDD; cd05008; SIS_GlmS_GlmD_1; 1.
DR CDD; cd05009; SIS_GlmS_GlmD_2; 1.
DR Gene3D; 3.60.20.10; Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1; 1.
DR InterPro; IPR017932; GATase_2_dom.
DR InterPro; IPR005855; GFAT.
DR InterPro; IPR047084; GFAT_N.
DR InterPro; IPR035466; GlmS/AgaS_SIS.
DR InterPro; IPR035490; GlmS/FrlB_SIS.
DR InterPro; IPR029055; Ntn_hydrolases_N.
DR InterPro; IPR001347; SIS_dom.
DR InterPro; IPR046348; SIS_dom_sf.
DR NCBIfam; TIGR01135; glmS; 1.
DR PANTHER; PTHR10937; GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING; 1.
DR PANTHER; PTHR10937:SF10; GLUTAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE [ISOMERIZING] 2; 1.
DR Pfam; PF13522; GATase_6; 1.
DR Pfam; PF01380; SIS; 2.
DR SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1.
DR SUPFAM; SSF53697; SIS domain; 1.
DR PROSITE; PS51278; GATASE_TYPE_2; 1.
DR PROSITE; PS51464; SIS; 2.
PE 4: Predicted;
KW Glutamine amidotransferase {ECO:0000256|ARBA:ARBA00022962};
KW Reference proteome {ECO:0000313|Proteomes:UP000005207};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 2..291
FT /note="Glutamine amidotransferase type-2"
FT /evidence="ECO:0000259|PROSITE:PS51278"
FT DOMAIN 363..502
FT /note="SIS"
FT /evidence="ECO:0000259|PROSITE:PS51464"
FT DOMAIN 534..675
FT /note="SIS"
FT /evidence="ECO:0000259|PROSITE:PS51464"
FT REGION 230..249
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 685 AA; 77202 MW; E7DC2EBEB9D647AE CRC64;
MCGIFAYLNY RVPRTRKEIF ETLVKGLQRL EYRGYDSAGI AVDGPSKTTS DQNNNSICLI
KKTGKVKALD EELYKKDSLN LDEELCTHFG IAHTRWATHG EPSALNSHPQ RSDKDNEFVV
IHNGIITNYK ELKKYLINKG YEFESETDTE VIPKLIKYIY DNRENDSITF SKLVERVIKQ
LEGAFALVFK SRHFPGEAVA TRRGSPMVIG VRSEHELLTE HIPVQYNSAH HKEEVHEKNT
RNRADSSSNI NSVGDGRAVE FYFASDASAI IEHTNKVLYM EDDDIAVVTG GKLSIHRMNR
QAGEDPVRAI MTLQMELQQI MKGNFDAFMQ KEIFEQPESV VNTMRGRICF DTKKVVLGGL
KDHLKEIKRC RRLIMIGCGT SFHAAVATRQ ILEELTELPV MVELASDFLD RITPVFRDDV
CFFISQSGET ADTLMALRYC KDRGALTVGV TNTVGSSICR ETDCGVHINA GPEIGVASTK
AYTSQFVSLI MFGLMMSEDR LSLQKRREEI INSLKMLPEL IKKVLSLDEK IKAIANELYQ
QRSLLVMGRG FNYATCLEGA LKIKEITYMH SEGILAGELK HGPLALVDKH MPVIMIIMRD
ACYTKCQNAL QQVTARSGRP IIICCQDDTE LTKNAYQTIE LPQTVDCLQG ILSVIPLQLM
SFHLAVLRGY DVDFPRNLAK SVTVE
//
