ID I3KEM2_ORENI Unreviewed; 324 AA.
AC I3KEM2;
DT 11-JUL-2012, integrated into UniProtKB/TrEMBL.
DT 17-JUN-2020, sequence version 2.
DT 27-MAR-2024, entry version 65.
DE RecName: Full=Mitochondrial glutamate carrier 1 {ECO:0000256|ARBA:ARBA00040262};
DE AltName: Full=Glutamate/H(+) symporter 1 {ECO:0000256|ARBA:ARBA00041922};
DE AltName: Full=Solute carrier family 25 member 22 {ECO:0000256|ARBA:ARBA00041877};
OS Oreochromis niloticus (Nile tilapia) (Tilapia nilotica).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Cichlomorphae; Cichliformes; Cichlidae; African cichlids;
OC Pseudocrenilabrinae; Oreochromini; Oreochromis.
OX NCBI_TaxID=8128 {ECO:0000313|Ensembl:ENSONIP00000019567.2, ECO:0000313|Proteomes:UP000005207};
RN [1] {ECO:0000313|Proteomes:UP000005207}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG Broad Institute Genome Assembly Team;
RG Broad Institute Sequencing Platform;
RA Di Palma F., Johnson J., Lander E.S., Lindblad-Toh K.;
RT "The Genome Sequence of Oreochromis niloticus (Nile Tilapia).";
RL Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSONIP00000019567.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(in) + L-glutamate(in) = H(+)(out) + L-glutamate(out);
CC Xref=Rhea:RHEA:70955, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985;
CC Evidence={ECO:0000256|ARBA:ARBA00036753};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}. Mitochondrion
CC inner membrane {ECO:0000256|ARBA:ARBA00004448}; Multi-pass membrane
CC protein {ECO:0000256|ARBA:ARBA00004448}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000256|ARBA:ARBA00006375, ECO:0000256|RuleBase:RU000488}.
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DR AlphaFoldDB; I3KEM2; -.
DR Ensembl; ENSONIT00000019584.2; ENSONIP00000019567.2; ENSONIG00000015550.2.
DR eggNOG; KOG0750; Eukaryota.
DR GeneTree; ENSGT00940000165631; -.
DR HOGENOM; CLU_015166_3_4_1; -.
DR TreeFam; TF313209; -.
DR Proteomes; UP000005207; Linkage group LG7.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR Gene3D; 1.50.40.10; Mitochondrial carrier domain; 1.
DR InterPro; IPR002067; Mit_carrier.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR PANTHER; PTHR45678; MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER 1-RELATED; 1.
DR PANTHER; PTHR45678:SF3; MITOCHONDRIAL GLUTAMATE CARRIER 1; 1.
DR Pfam; PF00153; Mito_carr; 3.
DR PRINTS; PR00926; MITOCARRIER.
DR SUPFAM; SSF103506; Mitochondrial carrier; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 3: Inferred from homology;
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|PROSITE-
KW ProRule:PRU00282}; Reference proteome {ECO:0000313|Proteomes:UP000005207};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|PROSITE-
KW ProRule:PRU00282}; Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|RuleBase:RU000488}.
FT TRANSMEM 12..29
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 293..319
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT REPEAT 6..93
FT /note="Solcar"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00282"
FT REPEAT 100..215
FT /note="Solcar"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00282"
FT REPEAT 224..313
FT /note="Solcar"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00282"
SQ SEQUENCE 324 AA; 35097 MW; 4DC647B5B60496A7 CRC64;
MADKQISLPA KLINGGIAGL IGVTCVFPID LAKTRLQNQQ NGSRLYTSMS DCLIKTIRSE
GYFGMYRGAA VNLTLVTPEK AIKLAANDFF RHHLSKDGKI TLFKEMLAGC GAGTCQVIVT
TPMEMLKIQL QDAGRIGKRR SNLAKTSYLV FFSVTNPKLA AGTVEMKSPT AMQITRQLLR
EKGIAGLYKG LGATLLRDVP FSIIYFPLFA NLNNFGKRGA EGPAPFYVSF ISGCVAGSTA
AVAVNPVDVI KTRLQSLTRG NTEDTYAGVT DCIRKIMRNE GPSAFLKGAY CRALVIAPLF
GIAQVVYFLG VGEFILSFLP KKDK
//
