ID I3KGH2_ORENI Unreviewed; 615 AA.
AC I3KGH2;
DT 11-JUL-2012, integrated into UniProtKB/TrEMBL.
DT 17-JUN-2020, sequence version 2.
DT 27-MAR-2024, entry version 63.
DE RecName: Full=Choline O-acetyltransferase {ECO:0000256|ARBA:ARBA00040495};
DE EC=2.3.1.6 {ECO:0000256|ARBA:ARBA00039091};
GN Name=CHAT {ECO:0000313|Ensembl:ENSONIP00000020217.2};
OS Oreochromis niloticus (Nile tilapia) (Tilapia nilotica).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Cichlomorphae; Cichliformes; Cichlidae; African cichlids;
OC Pseudocrenilabrinae; Oreochromini; Oreochromis.
OX NCBI_TaxID=8128 {ECO:0000313|Ensembl:ENSONIP00000020217.2, ECO:0000313|Proteomes:UP000005207};
RN [1] {ECO:0000313|Ensembl:ENSONIP00000020217.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Catalyzes the reversible synthesis of acetylcholine (ACh)
CC from acetyl CoA and choline at cholinergic synapses.
CC {ECO:0000256|ARBA:ARBA00037088}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + choline = acetylcholine + CoA;
CC Xref=Rhea:RHEA:18821, ChEBI:CHEBI:15354, ChEBI:CHEBI:15355,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.6;
CC Evidence={ECO:0000256|ARBA:ARBA00036720};
CC -!- SIMILARITY: Belongs to the carnitine/choline acetyltransferase family.
CC {ECO:0000256|ARBA:ARBA00005232, ECO:0000256|RuleBase:RU003801}.
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DR AlphaFoldDB; I3KGH2; -.
DR STRING; 8128.ENSONIP00000035085; -.
DR Ensembl; ENSONIT00000020234.2; ENSONIP00000020217.2; ENSONIG00000016047.2.
DR eggNOG; KOG3717; Eukaryota.
DR GeneTree; ENSGT01060000248556; -.
DR HOGENOM; CLU_013513_3_0_1; -.
DR TreeFam; TF313836; -.
DR Proteomes; UP000005207; Unplaced.
DR GO; GO:0016746; F:acyltransferase activity; IEA:UniProtKB-KW.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 1.
DR Gene3D; 3.30.559.70; Choline/Carnitine o-acyltransferase, domain 2; 1.
DR InterPro; IPR000542; Carn_acyl_trans.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR039551; Cho/carn_acyl_trans.
DR InterPro; IPR042231; Cho/carn_acyl_trans_2.
DR PANTHER; PTHR22589; CARNITINE O-ACYLTRANSFERASE; 1.
DR PANTHER; PTHR22589:SF14; CHOLINE O-ACETYLTRANSFERASE; 1.
DR Pfam; PF00755; Carn_acyltransf; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 2.
DR PROSITE; PS00439; ACYLTRANSF_C_1; 1.
DR PROSITE; PS00440; ACYLTRANSF_C_2; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315,
KW ECO:0000256|RuleBase:RU003801};
KW Neurotransmitter biosynthesis {ECO:0000256|ARBA:ARBA00022979};
KW Reference proteome {ECO:0000313|Proteomes:UP000005207};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU003801}.
FT DOMAIN 20..582
FT /note="Choline/carnitine acyltransferase"
FT /evidence="ECO:0000259|Pfam:PF00755"
FT ACT_SITE 328
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR600542-1"
SQ SEQUENCE 615 AA; 69235 MW; 7A7EF1C1A0DAE066 CRC64;
MPILERGTAR DQDSQVLPKV PVPLLKQTLD TYLKCVQHLV KEEQFKKTRA IVEKFGAPGG
VGETLQKKLL ERRDKMANWV YDYWLEDMYL NNRLALPVNS SPAMVFPKQM FKDHKDALRF
AARLISGVLE YKALIDGRSL PVDFTRGQLA VMPMCMEQYY RLFTSYRYPG LKTDTLTVQM
KAAFSAPEHM IVACKNQFFV LDVVANSKQL SEEEILSQLE KIVKMSENAE ERLPPFGILT
SDGRTEWAQA REALIKDQIN RDSLALIESC ICVVCLDEPS GLQPSDTNRA LLMLHGGGHE
KNGANRWYDK SMQFVVGMDG ICGVVCEHSP FEGIVLVQFS SLFRELPPPR RLRWKCNPHI
QGLLAASAER LQRLVNNLDM TVFKFRDYGK EFIKQQKMSP DAFIQVALQL AFFKCTGRLV
STYESASIRR FQEGRVDNIR SATAEALAFV KSMADERGTF SDSEKMKRLG DAIKAQTSYT
IAAITGMAID NHLLGLLRIS KELNMEKPEI FCDETYLASN HFILSTSQVP TTVEMFCCYG
PVVPNGYGAC YNPQSDHIIF CVSSFWDNTE TSSVVFVKAL NEGLLEIRDL CNRSSATASK
LAVASQGVAK EAVQM
//
