ID I3QMG6_HELAN Unreviewed; 328 AA.
AC I3QMG6;
DT 05-SEP-2012, integrated into UniProtKB/TrEMBL.
DT 05-SEP-2012, sequence version 1.
DT 22-FEB-2023, entry version 39.
DE RecName: Full=Acyl-[acyl-carrier-protein] desaturase {ECO:0000256|ARBA:ARBA00030374, ECO:0000256|RuleBase:RU000582};
DE EC=1.14.19.- {ECO:0000256|RuleBase:RU000582};
DE Flags: Fragment;
GN Name=fab2 {ECO:0000313|EMBL:AFK12526.1};
OS Helianthus annuus (Common sunflower).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Asteroideae;
OC Heliantheae alliance; Heliantheae; Helianthus.
OX NCBI_TaxID=4232 {ECO:0000313|EMBL:AFK12526.1};
RN [1] {ECO:0000313|EMBL:AFK12526.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=280 {ECO:0000313|EMBL:AFK12535.1}, 801
RC {ECO:0000313|EMBL:AFK12536.1}, AR {ECO:0000313|EMBL:AFK12521.1}, HA8
RC {ECO:0000313|EMBL:AFK12537.1}, HAV {ECO:0000313|EMBL:AFK12529.1}, HID
RC {ECO:0000313|EMBL:AFK12530.1}, HO {ECO:0000313|EMBL:AFK12531.1}, KS
RC {ECO:0000313|EMBL:AFK12523.1}, MAN {ECO:0000313|EMBL:AFK12532.1}, MEN
RC {ECO:0000313|EMBL:AFK12538.1}, MEX {ECO:0000313|EMBL:AFK12524.1}, MO
RC {ECO:0000313|EMBL:AFK12525.1}, NEG {ECO:0000313|EMBL:AFK12533.1}, OH
RC {ECO:0000313|EMBL:AFK12526.1}, PER {ECO:0000313|EMBL:AFK12539.1}, SD
RC {ECO:0000313|EMBL:AFK12527.1}, SEN {ECO:0000313|EMBL:AFK12534.1}, TN
RC {ECO:0000313|EMBL:AFK12528.1}, and VNI {ECO:0000313|EMBL:AFK12540.1};
RX PubMed=22580969; DOI=10.1007/s00122-012-1881-z;
RA Chapman M.A., Burke J.M.;
RT "Evidence of selection on fatty acid biosynthetic genes during the
RT evolution of cultivated sunflower.";
RL Theor. Appl. Genet. 125:897-907(2012).
CC -!- FUNCTION: Introduction of a cis double bond between carbons of the acyl
CC chain. {ECO:0000256|RuleBase:RU000582}.
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|PIRSR:PIRSR000346-1};
CC Note=Binds 2 iron ions per subunit. {ECO:0000256|PIRSR:PIRSR000346-1};
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000256|RuleBase:RU000582};
CC Note=Binds 2 Fe(2+) ions per subunit. {ECO:0000256|RuleBase:RU000582};
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738,
CC ECO:0000256|RuleBase:RU000582}.
CC -!- SUBCELLULAR LOCATION: Plastid {ECO:0000256|ARBA:ARBA00004474}.
CC -!- SIMILARITY: Belongs to the fatty acid desaturase type 2 family.
CC {ECO:0000256|ARBA:ARBA00008749, ECO:0000256|RuleBase:RU000582}.
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DR EMBL; JQ974514; AFK12521.1; -; Genomic_DNA.
DR EMBL; JQ974516; AFK12523.1; -; Genomic_DNA.
DR EMBL; JQ974517; AFK12524.1; -; Genomic_DNA.
DR EMBL; JQ974518; AFK12525.1; -; Genomic_DNA.
DR EMBL; JQ974519; AFK12526.1; -; Genomic_DNA.
DR EMBL; JQ974520; AFK12527.1; -; Genomic_DNA.
DR EMBL; JQ974521; AFK12528.1; -; Genomic_DNA.
DR EMBL; JQ974522; AFK12529.1; -; Genomic_DNA.
DR EMBL; JQ974523; AFK12530.1; -; Genomic_DNA.
DR EMBL; JQ974524; AFK12531.1; -; Genomic_DNA.
DR EMBL; JQ974525; AFK12532.1; -; Genomic_DNA.
DR EMBL; JQ974526; AFK12533.1; -; Genomic_DNA.
DR EMBL; JQ974527; AFK12534.1; -; Genomic_DNA.
DR EMBL; JQ974528; AFK12535.1; -; Genomic_DNA.
DR EMBL; JQ974529; AFK12536.1; -; Genomic_DNA.
DR EMBL; JQ974530; AFK12537.1; -; Genomic_DNA.
DR EMBL; JQ974531; AFK12538.1; -; Genomic_DNA.
DR EMBL; JQ974532; AFK12539.1; -; Genomic_DNA.
DR EMBL; JQ974533; AFK12540.1; -; Genomic_DNA.
DR AlphaFoldDB; I3QMG6; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-KW.
DR GO; GO:0045300; F:acyl-[acyl-carrier-protein] desaturase activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW.
DR CDD; cd01050; Acyl_ACP_Desat; 1.
DR Gene3D; 1.10.620.20; Ribonucleotide Reductase, subunit A; 1.
DR InterPro; IPR005803; FADS-2_CS.
DR InterPro; IPR005067; Fatty_acid_desaturase-2.
DR InterPro; IPR009078; Ferritin-like_SF.
DR InterPro; IPR012348; RNR-like.
DR PANTHER; PTHR31155; ACYL- ACYL-CARRIER-PROTEIN DESATURASE-RELATED; 1.
DR PANTHER; PTHR31155:SF9; STEAROYL-[ACYL-CARRIER-PROTEIN] 9-DESATURASE 7, CHLOROPLASTIC; 1.
DR Pfam; PF03405; FA_desaturase_2; 1.
DR PIRSF; PIRSF000346; Dlt9_acylACP_des; 1.
DR SUPFAM; SSF47240; Ferritin-like; 1.
DR PROSITE; PS00574; FATTY_ACID_DESATUR_2; 1.
PE 3: Inferred from homology;
KW Chloroplast {ECO:0000256|RuleBase:RU000582};
KW Fatty acid biosynthesis {ECO:0000256|ARBA:ARBA00023160,
KW ECO:0000256|RuleBase:RU000582};
KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Iron {ECO:0000256|PIRSR:PIRSR000346-1};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516,
KW ECO:0000256|RuleBase:RU000582};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR000346-1};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000256|RuleBase:RU000582}; Plastid {ECO:0000256|RuleBase:RU000582};
KW Transit peptide {ECO:0000256|ARBA:ARBA00022946}.
FT BINDING 90
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR000346-1"
FT BINDING 128
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR000346-1"
FT BINDING 128
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR000346-1"
FT BINDING 131
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR000346-1"
FT BINDING 171
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR000346-1"
FT BINDING 204
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR000346-1"
FT BINDING 204
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR000346-1"
FT BINDING 207
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR000346-1"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:AFK12526.1"
FT NON_TER 328
FT /evidence="ECO:0000313|EMBL:AFK12526.1"
SQ SEQUENCE 328 AA; 37487 MW; 55CCA1AA6F53E103 CRC64;
KPFTPPREVH QQVLHSMPPQ KIEIFKSMEG WAEDNILVHL KPVEKCWQAQ DFLPDPASDG
FMEQVEELRA RAKEIPDDYF VVLVGDMITE EALPTYQTML NTLDGVRDET GASPTSWAIW
TRAWTAEENR HGDLLHQYLY LSGRVDMRQI QKTIQYLIGP YLGFIYTSFQ ERATFISHGN
TARHAKEHGD VKLAQMCGII AADEKRHETA YTKIVEKLFE IDPDGTVLAF ADMMRKKISM
PAHLMYDGRD DNLFENFSAV AQRLGVYTAK DYADILEFLV GRWKVADLTG LSGEGRKAQD
YVCGLAPRIR RLEERNSARA KESVNVPF
//
