ID I6CD23_SHIFL Unreviewed; 246 AA.
AC I6CD23;
DT 05-SEP-2012, integrated into UniProtKB/TrEMBL.
DT 05-SEP-2012, sequence version 1.
DT 24-JAN-2024, entry version 49.
DE RecName: Full=UDP-N-acetyl-D-mannosaminuronic acid transferase {ECO:0000256|HAMAP-Rule:MF_01001};
DE Short=UDP-ManNAcA transferase {ECO:0000256|HAMAP-Rule:MF_01001};
DE EC=2.4.1.180 {ECO:0000256|HAMAP-Rule:MF_01001};
GN Name=wecG {ECO:0000256|HAMAP-Rule:MF_01001};
GN Synonyms=rffM {ECO:0000256|HAMAP-Rule:MF_01001};
GN ORFNames=SFK315_4245 {ECO:0000313|EMBL:EIQ17415.1};
OS Shigella flexneri K-315.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=766150 {ECO:0000313|EMBL:EIQ17415.1, ECO:0000313|Proteomes:UP000005407};
RN [1] {ECO:0000313|EMBL:EIQ17415.1, ECO:0000313|Proteomes:UP000005407}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K-315 {ECO:0000313|EMBL:EIQ17415.1,
RC ECO:0000313|Proteomes:UP000005407};
RA Rasko D., Redman J., Daugherty S.C., Tallon L., Sadzewicz L., Jones K.,
RA Santana-Cruz I., Liu X.;
RL Submitted (MAR-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA (Lipid II),
CC the second lipid-linked intermediate involved in enterobacterial common
CC antigen (ECA) synthesis. {ECO:0000256|HAMAP-Rule:MF_01001}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N-acetyl-alpha-D-glucosaminyl-di-trans,octa-cis-undecaprenyl
CC diphosphate + UDP-N-acetyl-alpha-D-mannosaminouronate = beta-D-
CC ManNAcA-(1->4)-alpha-D-GlcNAc-di-trans,octa-cis-undecaprenyl
CC diphosphate + H(+) + UDP; Xref=Rhea:RHEA:28366, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:61495, ChEBI:CHEBI:62959,
CC ChEBI:CHEBI:70731; EC=2.4.1.180; Evidence={ECO:0000256|HAMAP-
CC Rule:MF_01001};
CC -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC antigen biosynthesis. {ECO:0000256|HAMAP-Rule:MF_01001}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 26 family.
CC {ECO:0000256|HAMAP-Rule:MF_01001}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EIQ17415.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AKMY01000070; EIQ17415.1; -; Genomic_DNA.
DR AlphaFoldDB; I6CD23; -.
DR PATRIC; fig|766150.3.peg.4090; -.
DR UniPathway; UPA00566; -.
DR Proteomes; UP000005407; Unassembled WGS sequence.
DR GO; GO:0047241; F:lipopolysaccharide N-acetylmannosaminouronosyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd06533; Glyco_transf_WecG_TagA; 1.
DR HAMAP; MF_01001; WecG_RffM; 1.
DR InterPro; IPR023085; UDP-ManNAcA_Trfase_WecG.
DR InterPro; IPR004629; WecG_TagA_CpsF.
DR NCBIfam; TIGR00696; wecG_tagA_cpsF; 1.
DR PANTHER; PTHR34136; -; 1.
DR PANTHER; PTHR34136:SF1; UDP-N-ACETYL-D-MANNOSAMINURONIC ACID TRANSFERASE; 1.
DR Pfam; PF03808; Glyco_tran_WecG; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase {ECO:0000256|HAMAP-Rule:MF_01001,
KW ECO:0000313|EMBL:EIQ17415.1};
KW Transferase {ECO:0000256|HAMAP-Rule:MF_01001, ECO:0000313|EMBL:EIQ17415.1}.
SQ SEQUENCE 246 AA; 27943 MW; E84F5C7834354728 CRC64;
MNNNTTAPTY TLRGLQLIGW RDMQHALDYL FADGQLKQGT LVAINAEKML TIEDNAEVRE
LINAAEFKYA DGISVVHSVR KKYPQAQVSR VAGADLWEEL MARAGKEGTP VFLVGGKPEV
LAQTEAKLRN QWNVNIVGSQ DGYFKPEQRQ ALFERIHASG AQIVTVAMGS PKQEIFMRDC
RLVHPDALYM GVGGTYDVFT GHVKRAPKIW QTLGLEWLYR LLSQPSRIKR QLRLLRYLRW
HYTGNL
//
