ID I6S949_9ROSI Unreviewed; 392 AA.
AC I6S949;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=trihydroxystilbene synthase {ECO:0000256|ARBA:ARBA00044056};
DE EC=2.3.1.95 {ECO:0000256|ARBA:ARBA00044056};
OS Vitis quinquangularis.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; Vitales; Vitaceae; Viteae; Vitis.
OX NCBI_TaxID=239086 {ECO:0000313|EMBL:AFM56636.1};
RN [1] {ECO:0000313|EMBL:AFM56636.1}
RP NUCLEOTIDE SEQUENCE.
RA Wang Y.J., He M.Y., Cao J.L.;
RL Submitted (MAR-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:AFM56636.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=24246671; DOI=10.1016/j.plaphy.2013.10.021;
RA Shi J., He M., Cao J., Wang H., Ding J., Jiao Y., Li R., He J., Wang D.,
RA Wang Y.;
RT "The comparative analysis of the potential relationship between resveratrol
RT and stilbene synthase gene family in the development stages of grapes
RT (Vitis quinquangularis and Vitis vinifera).";
RL Plant Physiol. Biochem. 74:24-32(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-coumaroyl-CoA + 3 H(+) + 3 malonyl-CoA = 4 CO2 + 4 CoA +
CC trans-resveratrol; Xref=Rhea:RHEA:11936, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:45713, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57355, ChEBI:CHEBI:57384; EC=2.3.1.95;
CC Evidence={ECO:0000256|ARBA:ARBA00001104};
CC -!- PATHWAY: Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene
CC biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step
CC 2/2. {ECO:0000256|ARBA:ARBA00043953}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000256|ARBA:ARBA00005531,
CC ECO:0000256|RuleBase:RU003633}.
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DR EMBL; JQ868659; AFM56636.1; -; mRNA.
DR EMBL; JQ868670; AFM56647.1; -; mRNA.
DR AlphaFoldDB; I6S949; -.
DR GO; GO:0050350; F:trihydroxystilbene synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR CDD; cd00831; CHS_like; 1.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR InterPro; IPR011141; Polyketide_synthase_type-III.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR11877:SF14; CHALCONE SYNTHASE; 1.
DR PANTHER; PTHR11877; HYDROXYMETHYLGLUTARYL-COA SYNTHASE; 1.
DR Pfam; PF02797; Chal_sti_synt_C; 1.
DR Pfam; PF00195; Chal_sti_synt_N; 1.
DR PIRSF; PIRSF000451; PKS_III; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase {ECO:0000256|RuleBase:RU003633};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Plant defense {ECO:0000256|ARBA:ARBA00022821};
KW Stress response {ECO:0000256|ARBA:ARBA00023016};
KW Transferase {ECO:0000256|RuleBase:RU003633}.
FT DOMAIN 5..228
FT /note="Chalcone/stilbene synthase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00195"
FT DOMAIN 238..388
FT /note="Chalcone/stilbene synthase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02797"
FT ACT_SITE 164
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000451-1"
SQ SEQUENCE 392 AA; 42778 MW; E0A7561685AAA447 CRC64;
MASVEEIRNA QRAKGPATIL AIGTATPDHC VYQSDYADYY FRVTKSEHMT ELKKKFNRIC
DKSMIKKRYI HLTEEMLEEH PNIGAYMAPS LNIRQEIITA EVPKLGKEAA LKALKEWGQP
KSKITHLVFC TTSGVEMPGA DYKLANLLGL ETSVRRVMLY HQGCYAGGTV LRTAKDLAEN
NVGARVLVVC SEITVVTFRG PSEDALDSLV GQALFGDGSA AVIVGSDPDV SIERPLFQLV
SAAQTFIPNS AGAIAGNLRE VGLTFHLWPN VPTLISENIE KCLSQAFDPL GISDWNSLFW
IAHPGGPAIL DAVEAKLNLE KKKLEATRHV LSEYGNMSSA CVLFILDEMR KKSLKGENAT
TGEGLDWGVL FGFGPGLTIE TVVLHSIPMV SN
//
