ID I6TVJ5_9EURY Unreviewed; 773 AA.
AC I6TVJ5;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 27-MAR-2024, entry version 54.
DE RecName: Full=Carbamoyltransferase {ECO:0000256|PIRNR:PIRNR006256};
DE EC=6.2.-.- {ECO:0000256|PIRNR:PIRNR006256};
GN ORFNames=PFC_01925 {ECO:0000313|EMBL:AFN03354.1};
OS Pyrococcus furiosus COM1.
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=1185654 {ECO:0000313|Proteomes:UP000006216};
RN [1] {ECO:0000313|EMBL:AFN03354.1, ECO:0000313|Proteomes:UP000006216}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=COM1 {ECO:0000313|EMBL:AFN03354.1,
RC ECO:0000313|Proteomes:UP000006216};
RX PubMed=22636780; DOI=10.1128/JB.00439-12;
RA Bridger S.L., Lancaster W.A., Poole F.L.II., Schut G.J., Adams M.W.;
RT "Genome Sequencing of a Genetically-Tractable Pyrococcus furiosus Strain
RT Reveals a Highly Dynamic Genome.";
RL J. Bacteriol. 194:4097-4106(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + C-terminal L-cysteinyl-[HypE protein] + carbamoyl
CC phosphate + H2O = AMP + C-terminal S-carboxamide-L-cysteinyl-[HypE
CC protein] + diphosphate + H(+) + phosphate; Xref=Rhea:RHEA:55636,
CC Rhea:RHEA-COMP:14247, Rhea:RHEA-COMP:14392, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58228, ChEBI:CHEBI:76913,
CC ChEBI:CHEBI:139126, ChEBI:CHEBI:456215;
CC Evidence={ECO:0000256|ARBA:ARBA00001186};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an acyl phosphate + H2O = a carboxylate + H(+) + phosphate;
CC Xref=Rhea:RHEA:14965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29067, ChEBI:CHEBI:43474, ChEBI:CHEBI:59918; EC=3.6.1.7;
CC Evidence={ECO:0000256|PROSITE-ProRule:PRU00520};
CC -!- PATHWAY: Protein modification; [NiFe] hydrogenase maturation.
CC {ECO:0000256|ARBA:ARBA00004711}.
CC -!- SIMILARITY: Belongs to the carbamoyltransferase HypF family.
CC {ECO:0000256|ARBA:ARBA00008097, ECO:0000256|PIRNR:PIRNR006256}.
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DR EMBL; CP003685; AFN03354.1; -; Genomic_DNA.
DR RefSeq; WP_014835167.1; NC_018092.1.
DR AlphaFoldDB; I6TVJ5; -.
DR GeneID; 41712364; -.
DR KEGG; pfi:PFC_01925; -.
DR PATRIC; fig|1185654.4.peg.393; -.
DR HOGENOM; CLU_009164_0_0_2; -.
DR UniPathway; UPA00335; -.
DR Proteomes; UP000006216; Chromosome.
DR GO; GO:0003998; F:acylphosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0016743; F:carboxyl- or carbamoyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003725; F:double-stranded RNA binding; IEA:InterPro.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR Gene3D; 3.30.110.120; -; 1.
DR Gene3D; 3.30.420.360; -; 1.
DR Gene3D; 3.30.420.40; -; 1.
DR Gene3D; 3.90.870.50; -; 1.
DR InterPro; IPR001792; Acylphosphatase-like_dom.
DR InterPro; IPR036046; Acylphosphatase-like_dom_sf.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR004421; Carbamoyltransferase_HypF.
DR InterPro; IPR017945; DHBP_synth_RibB-like_a/b_dom.
DR InterPro; IPR041440; HypF_C.
DR InterPro; IPR006070; Sua5-like_dom.
DR InterPro; IPR011125; Znf_HypF.
DR NCBIfam; TIGR00143; hypF; 1.
DR PANTHER; PTHR42959; CARBAMOYLTRANSFERASE; 1.
DR PANTHER; PTHR42959:SF1; CARBAMOYLTRANSFERASE HYPF; 1.
DR Pfam; PF00708; Acylphosphatase; 1.
DR Pfam; PF17788; HypF_C; 1.
DR Pfam; PF01300; Sua5_yciO_yrdC; 1.
DR Pfam; PF07503; zf-HYPF; 2.
DR PIRSF; PIRSF006256; CMPcnvr_hdrg_mat; 1.
DR SUPFAM; SSF53067; Actin-like ATPase domain; 2.
DR SUPFAM; SSF54975; Acylphosphatase/BLUF domain-like; 1.
DR SUPFAM; SSF55821; YrdC/RibB; 1.
DR PROSITE; PS51160; ACYLPHOSPHATASE_3; 1.
DR PROSITE; PS51163; YRDC; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|PROSITE-ProRule:PRU00520};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771}.
FT DOMAIN 3..90
FT /note="Acylphosphatase-like"
FT /evidence="ECO:0000259|PROSITE:PS51160"
FT DOMAIN 201..387
FT /note="YrdC-like"
FT /evidence="ECO:0000259|PROSITE:PS51163"
FT ACT_SITE 18
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00520"
FT ACT_SITE 36
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00520"
SQ SEQUENCE 773 AA; 87411 MW; 101186E44648F899 CRC64;
MKAYRIHVQG IVQAVGFRPF VYRIAHAHNL RGYVRNLGDA GVEIVVEGRE EDIEAFIKDL
YKKKPPLARI DKVEREEIPL QGFDRFYIEK SSTEKKGEGD SIIPPDIAIC EDCLRELFNP
TDKRYMYPFI VCTNCGPRFT IIEDLPYDRE NTAMREFPMC EFCRSEYEDP LNRRYHAEPV
ACPTCGPSYR LYTSDGNEII GDPLRKAAKL IDKGYIVAIK GIGGIHLACD ATREDVVAEL
RKRIFRPQKP FAIMAKDLET VRTFAYISPE EEEELTSYRR PIVALKKKEP FPLPENLAPG
LHTIGVMLPY AGTHYILFHW SKTPVYVMTS ANFPGMPMIK DNEEAFEKLR DVADYLLLHN
RRIPNRADDS VVRFVDGRRA VIRRSRGFVP LGIEIPFEYK GLAVGAELMN AFGVVKNGKV
YPSQYIGDTS KIEVLEFMRE AVRHFFKILR VDNLDLVVAD LHPSYNTTKL GMEIAEEFGA
EFLQVQHHYA HVASVMAEHN LEEVVGIALD GVGYGTDGKT WGGEVIYLSY EDVERLAHIE
YYPLPGGDLA SYYPLRALIG ILSLNHDLEE VEKIIREFCP NAIKSLKYGE TEFRVIMRQL
SSGINVAYAS STGRVLDAFS VLLNVSYRRH YEGEPAMKLE SFAYQGKNDL KLTAPIEGEE
IKVSELFEEV LELMGKANPK DIAYSVHLAL ARAFAEVSVE KAKEFGAKTV VLGGGVGYNE
LIVKTIRKIV EGRGLRFLTT YEVPRGDNGI NVGQAFLGGL YLEGYLNRED LSI
//
