ID I7BYJ7_PSEPT Unreviewed; 69 AA.
AC I7BYJ7;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 27-MAR-2024, entry version 44.
DE RecName: Full=2-hydroxymuconate tautomerase {ECO:0000256|ARBA:ARBA00015750, ECO:0000256|RuleBase:RU362032};
DE EC=5.3.2.6 {ECO:0000256|ARBA:ARBA00012667, ECO:0000256|RuleBase:RU362032};
DE AltName: Full=4-oxalocrotonate tautomerase {ECO:0000256|ARBA:ARBA00029674, ECO:0000256|RuleBase:RU362032};
GN OrderedLocusNames=T1E_3411 {ECO:0000313|EMBL:AFO49247.1};
OS Pseudomonas putida (strain DOT-T1E).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=1196325 {ECO:0000313|EMBL:AFO49247.1, ECO:0000313|Proteomes:UP000006503};
RN [1] {ECO:0000313|Proteomes:UP000006503}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DOT-T1E {ECO:0000313|Proteomes:UP000006503};
RX PubMed=23815283; DOI=10.1111/1751-7915.12061;
RA Udaondo Z., Molina L., Daniels C., Gomez M.J., Molina-Henares M.A.,
RA Matilla M.A., Roca A., Fernandez M., Duque E., Segura A., Ramos J.L.;
RT "Metabolic potential of the organic-solvent tolerant Pseudomonas putida
RT DOT-T1E deduced from its annotated genome.";
RL Microb. Biotechnol. 6:598-611(2013).
CC -!- FUNCTION: Catalyzes the ketonization of 2-hydroxymuconate
CC stereoselectively to yield 2-oxo-3-hexenedioate.
CC {ECO:0000256|ARBA:ARBA00003024}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2Z,4E)-2-hydroxyhexa-2,4-dienedioate = (3E)-2-oxohex-3-
CC enedioate; Xref=Rhea:RHEA:33431, ChEBI:CHEBI:28080,
CC ChEBI:CHEBI:64908; EC=5.3.2.6;
CC Evidence={ECO:0000256|ARBA:ARBA00001379,
CC ECO:0000256|RuleBase:RU362032};
CC -!- SUBUNIT: Homohexamer. {ECO:0000256|ARBA:ARBA00011643,
CC ECO:0000256|RuleBase:RU362032}.
CC -!- SIMILARITY: Belongs to the 4-oxalocrotonate tautomerase family.
CC {ECO:0000256|ARBA:ARBA00006723, ECO:0000256|RuleBase:RU362032}.
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DR EMBL; CP003734; AFO49247.1; -; Genomic_DNA.
DR RefSeq; WP_014753565.1; NZ_CP110782.1.
DR AlphaFoldDB; I7BYJ7; -.
DR KEGG; ppx:T1E_3411; -.
DR PATRIC; fig|1196325.3.peg.3381; -.
DR HOGENOM; CLU_148073_1_2_6; -.
DR Proteomes; UP000006503; Chromosome.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.30.429.10; Macrophage Migration Inhibitory Factor; 1.
DR InterPro; IPR018191; 4-OT.
DR InterPro; IPR004370; 4-OT-like_dom.
DR InterPro; IPR014347; Tautomerase/MIF_sf.
DR NCBIfam; TIGR00013; taut; 1.
DR PANTHER; PTHR35530:SF1; TAUTOMERASE BSL7456-RELATED; 1.
DR PANTHER; PTHR35530; TAUTOMERASE-RELATED; 1.
DR Pfam; PF01361; Tautomerase; 1.
DR SUPFAM; SSF55331; Tautomerase/MIF; 1.
PE 3: Inferred from homology;
KW Aromatic hydrocarbons catabolism {ECO:0000256|ARBA:ARBA00022797,
KW ECO:0000256|RuleBase:RU362032}; Isomerase {ECO:0000256|RuleBase:RU362032}.
FT DOMAIN 2..57
FT /note="4-oxalocrotonate tautomerase-like"
FT /evidence="ECO:0000259|Pfam:PF01361"
FT ACT_SITE 2
FT /note="Proton acceptor; via imino nitrogen"
FT /evidence="ECO:0000256|PIRSR:PIRSR618191-1"
SQ SEQUENCE 69 AA; 7895 MW; 826AAD39E5301089 CRC64;
MPFVSVRITR DGVTRDQKAQ VIKEITETLQ RVLGKKPELT HIVIEEVDTD NWGYAGMTTT
EYRSKKTQG
//
