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Database: UniProt
Entry: I7KDB2_METBM
LinkDB: I7KDB2_METBM
Original site: I7KDB2_METBM 
ID   I7KDB2_METBM            Unreviewed;       568 AA.
AC   I7KDB2;
DT   03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT   03-OCT-2012, sequence version 1.
DT   25-OCT-2017, entry version 31.
DE   RecName: Full=Methyl-coenzyme M reductase subunit alpha {ECO:0000256|PIRNR:PIRNR000262};
DE            EC=2.8.4.1 {ECO:0000256|PIRNR:PIRNR000262};
GN   Name=mcrA3 {ECO:0000313|EMBL:CCJ36661.1};
GN   OrderedLocusNames=BN140_1738 {ECO:0000313|EMBL:CCJ36661.1};
OS   Methanoculleus bourgensis (strain ATCC 43281 / DSM 3045 / OCM 15 /
OS   MS2) (Methanogenium bourgense).
OC   Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
OC   Methanomicrobiaceae; Methanoculleus.
OX   NCBI_TaxID=1201294 {ECO:0000313|EMBL:CCJ36661.1, ECO:0000313|Proteomes:UP000009007};
RN   [1] {ECO:0000313|Proteomes:UP000009007}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43281 / DSM 3045 / OCM 15 / MS2
RC   {ECO:0000313|Proteomes:UP000009007};
RX   PubMed=22965103; DOI=10.1128/JB.01292-12;
RA   Maus I., Wibberg D., Stantscheff R., Eikmeyer F.G., Seffner A.,
RA   Boelter J., Szczepanowski R., Blom J., Jaenicke S., Konig H.,
RA   Puhler A., Schluter A.;
RT   "Complete genome sequence of the hydrogenotrophic, methanogenic
RT   archaeon Methanoculleus bourgensis strain MS2T, isolated from a sewage
RT   sludge digester.";
RL   J. Bacteriol. 194:5487-5488(2012).
CC   -!- FUNCTION: Reduction of methyl-coenzyme M (2-(methylthio)
CC       ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate
CC       to methane and a heterodisulfide. {ECO:0000256|PIRNR:PIRNR000262}.
CC   -!- CATALYTIC ACTIVITY: Methyl-CoM + CoB = CoM-S-S-CoB + methane.
CC       {ECO:0000256|PIRNR:PIRNR000262}.
CC   -!- COFACTOR:
CC       Name=coenzyme F430; Xref=ChEBI:CHEBI:60540;
CC         Evidence={ECO:0000256|PIRNR:PIRNR000262};
CC       Note=Binds 1 coenzyme F430 noncovalently per subunit. Coenzyme
CC       F430 is a yellow nickel porphinoid.
CC       {ECO:0000256|PIRNR:PIRNR000262};
CC   -!- PATHWAY: One-carbon metabolism; methyl-coenzyme M reduction;
CC       methane from methyl-coenzyme M: step 1/1.
CC       {ECO:0000256|PIRNR:PIRNR000262}.
CC   -!- SUBUNIT: Hexamer of two alpha, two beta, and two gamma chains.
CC       {ECO:0000256|PIRNR:PIRNR000262}.
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DR   EMBL; HE964772; CCJ36661.1; -; Genomic_DNA.
DR   RefSeq; WP_014867635.1; NC_018227.2.
DR   EnsemblBacteria; CCJ36661; CCJ36661; BN140_1738.
DR   GeneID; 13355127; -.
DR   KEGG; mbg:BN140_1738; -.
DR   PATRIC; fig|1201294.9.peg.1906; -.
DR   KO; K00399; -.
DR   OMA; GRVCDGG; -.
DR   OrthoDB; POG093Z00ZI; -.
DR   BioCyc; MBOU1201294:GLGD-1742-MONOMER; -.
DR   UniPathway; UPA00646; UER00699.
DR   Proteomes; UP000009007; Chromosome.
DR   GO; GO:0050524; F:coenzyme-B sulfoethylthiotransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0015948; P:methanogenesis; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.840.10; -; 1.
DR   Gene3D; 3.30.70.470; -; 1.
DR   Gene3D; 3.90.390.10; -; 1.
DR   InterPro; IPR016212; Me_CoM_Rdtase_asu.
DR   InterPro; IPR008924; Me_CoM_Rdtase_asu/bsu_C.
DR   InterPro; IPR009047; Me_CoM_Rdtase_asu_C.
DR   InterPro; IPR003183; Me_CoM_Rdtase_asu_N.
DR   InterPro; IPR015811; Me_CoM_Rdtase_asu_N_sub1.
DR   InterPro; IPR015823; Me_CoM_Rdtase_asu_N_sub2.
DR   InterPro; IPR009024; Me_CoM_Rdtase_Fd-like_fold.
DR   Pfam; PF02249; MCR_alpha; 1.
DR   Pfam; PF02745; MCR_alpha_N; 1.
DR   PIRSF; PIRSF000262; MCR_alpha; 1.
DR   SUPFAM; SSF48081; SSF48081; 1.
DR   SUPFAM; SSF55088; SSF55088; 1.
DR   TIGRFAMs; TIGR03256; met_CoM_red_alp; 1.
PE   4: Predicted;
KW   Complete proteome {ECO:0000313|Proteomes:UP000009007};
KW   Metal-binding {ECO:0000256|PIRNR:PIRNR000262,
KW   ECO:0000256|PIRSR:PIRSR000262-1};
KW   Methanogenesis {ECO:0000256|PIRNR:PIRNR000262};
KW   Nickel {ECO:0000256|PIRNR:PIRNR000262, ECO:0000256|PIRSR:PIRSR000262-
KW   1}; Reference proteome {ECO:0000313|Proteomes:UP000009007};
KW   Transferase {ECO:0000256|PIRNR:PIRNR000262,
KW   ECO:0000313|EMBL:CCJ36661.1}.
FT   DOMAIN        8    278       MCR_alpha_N. {ECO:0000259|Pfam:PF02745}.
FT   DOMAIN      326    459       MCR_alpha. {ECO:0000259|Pfam:PF02249}.
FT   METAL       157    157       Nickel. {ECO:0000256|PIRSR:PIRSR000262-
FT                                1}.
FT   MOD_RES     267    267       Pros-methylhistidine. {ECO:0000256|PIRSR:
FT                                PIRSR000262-2}.
FT   MOD_RES     281    281       5-methylarginine. {ECO:0000256|PIRSR:
FT                                PIRSR000262-2}.
SQ   SEQUENCE   568 AA;  62754 MW;  8BA7537F892D1719 CRC64;
     MAKIERSQKL FLKALKEKFQ GQDVQSDTAE YYKYNGVRQS PRKMEFMKAS RAIEMDRGIS
     MYDPERAHLG GIPMGQRQLM TYEVSGTGVF VEGDDLHFVN NAAMQQFWDD IRRTVIVNMD
     LAHQTLQKRL GKEVTPETIN EYLHILNHAM PGAAVVQEHM VETHPGLVDD CYVKVFTGDQ
     ELADDLEPQF VLDVEKLFPA KQAEALSAAV GKSLWQAIHI PTAVSRTCDG GTTSRWSAMQ
     IGMSFIAAYR MCAGEAAVAD LSFAAKHAGV IQMASLLPAR RARGPNEPGG LAFGLFSDIV
     QTNRKYPNDP AKASLEVVGA GTMLYDQIWL GSYMSGGVGF TQYATAAYTD NILDEFTYYG
     MDYIKDKYKV DWKNPSANDK VKPTYDIVND MATEVTLNAM EQYEQFPTMM EDHFGGSQRA
     GVIAAASGLT TAIATGNSNA GLNGWYLSML LHKDGWSRLG FFGYDLQDQC GSANSLSIRG
     DEGAIGELRG PNYPNYAMNV GHQGEYAAIV GGSHYGRGDA WCFDPRVKIC FADPALKFDF
     AEPRREFAKG AIREFMPAGE RSLIIPAR
//
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