ID I7M407_TETTS Unreviewed; 1047 AA.
AC I7M407;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 2.
DT 27-MAR-2024, entry version 53.
DE RecName: Full=E1 ubiquitin-activating enzyme {ECO:0000256|ARBA:ARBA00012990};
DE EC=6.2.1.45 {ECO:0000256|ARBA:ARBA00012990};
GN ORFNames=TTHERM_00241900 {ECO:0000313|EMBL:EAS04669.2};
OS Tetrahymena thermophila (strain SB210).
OC Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata;
OC Oligohymenophorea; Hymenostomatida; Tetrahymenina; Tetrahymenidae;
OC Tetrahymena.
OX NCBI_TaxID=312017 {ECO:0000313|EMBL:EAS04669.2, ECO:0000313|Proteomes:UP000009168};
RN [1] {ECO:0000313|Proteomes:UP000009168}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SB210 {ECO:0000313|Proteomes:UP000009168};
RX PubMed=16933976; DOI=10.1371/journal.pbio.0040286;
RA Eisen J.A., Coyne R.S., Wu M., Wu D., Thiagarajan M., Wortman J.R.,
RA Badger J.H., Ren Q., Amedeo P., Jones K.M., Tallon L.J., Delcher A.L.,
RA Salzberg S.L., Silva J.C., Haas B.J., Majoros W.H., Farzad M.,
RA Carlton J.M., Smith R.K. Jr., Garg J., Pearlman R.E., Karrer K.M., Sun L.,
RA Manning G., Elde N.C., Turkewitz A.P., Asai D.J., Wilkes D.E., Wang Y.,
RA Cai H., Collins K., Stewart B.A., Lee S.R., Wilamowska K., Weinberg Z.,
RA Ruzzo W.L., Wloga D., Gaertig J., Frankel J., Tsao C.-C., Gorovsky M.A.,
RA Keeling P.J., Waller R.F., Patron N.J., Cherry J.M., Stover N.A.,
RA Krieger C.J., del Toro C., Ryder H.F., Williamson S.C., Barbeau R.A.,
RA Hamilton E.P., Orias E.;
RT "Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a
RT model eukaryote.";
RL PLoS Biol. 4:1620-1642(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine
CC = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-
CC L-cysteine.; EC=6.2.1.45; Evidence={ECO:0000256|ARBA:ARBA00000488};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the ubiquitin-activating E1 family.
CC {ECO:0000256|ARBA:ARBA00005673, ECO:0000256|RuleBase:RU000519}.
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DR EMBL; GG662443; EAS04669.2; -; Genomic_DNA.
DR RefSeq; XP_001024914.2; XM_001024914.2.
DR AlphaFoldDB; I7M407; -.
DR STRING; 312017.I7M407; -.
DR GeneID; 7825316; -.
DR KEGG; tet:TTHERM_00241900; -.
DR eggNOG; KOG2012; Eukaryota.
DR InParanoid; I7M407; -.
DR OrthoDB; 20494at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000009168; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008641; F:ubiquitin-like modifier activating enzyme activity; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 2.40.30.180; Ubiquitin-activating enzyme E1, FCCH domain; 1.
DR Gene3D; 3.50.50.80; Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 1; 1.
DR Gene3D; 3.40.50.12550; Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 2; 1.
DR Gene3D; 1.10.10.2660; Ubiquitin-activating enzyme E1, SCCH domain; 1.
DR Gene3D; 3.10.290.60; Ubiquitin-activating enzyme E1, UFD domain; 1.
DR InterPro; IPR032420; E1_4HB.
DR InterPro; IPR032418; E1_FCCH.
DR InterPro; IPR042302; E1_FCCH_sf.
DR InterPro; IPR045886; ThiF/MoeB/HesA.
DR InterPro; IPR000594; ThiF_NAD_FAD-bd.
DR InterPro; IPR018965; Ub-activating_enz_E1_C.
DR InterPro; IPR042449; Ub-E1_IAD_1.
DR InterPro; IPR038252; UBA_E1_C_sf.
DR InterPro; IPR019572; UBA_E1_SCCH.
DR InterPro; IPR042063; Ubi_acti_E1_SCCH.
DR InterPro; IPR035985; Ubiquitin-activating_enz.
DR InterPro; IPR018075; UBQ-activ_enz_E1.
DR InterPro; IPR033127; UBQ-activ_enz_E1_Cys_AS.
DR InterPro; IPR000011; UBQ/SUMO-activ_enz_E1-like.
DR NCBIfam; TIGR01408; Ube1; 1.
DR PANTHER; PTHR10953:SF4; UBA_E1_C DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR10953; UBIQUITIN-ACTIVATING ENZYME E1; 1.
DR Pfam; PF16191; E1_4HB; 1.
DR Pfam; PF16190; E1_FCCH; 1.
DR Pfam; PF09358; E1_UFD; 1.
DR Pfam; PF00899; ThiF; 2.
DR Pfam; PF10585; UBA_E1_SCCH; 1.
DR PRINTS; PR01849; UBIQUITINACT.
DR SMART; SM00985; UBA_e1_C; 1.
DR SUPFAM; SSF69572; Activating enzymes of the ubiquitin-like proteins; 2.
DR PROSITE; PS00865; UBIQUITIN_ACTIVAT_2; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|RuleBase:RU000519};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU000519};
KW Nucleotide-binding {ECO:0000256|RuleBase:RU000519};
KW Reference proteome {ECO:0000313|Proteomes:UP000009168};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|RuleBase:RU000519}.
FT DOMAIN 907..1042
FT /note="Ubiquitin-activating enzyme E1 C-terminal"
FT /evidence="ECO:0000259|SMART:SM00985"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 10..26
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 614
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10132"
SQ SEQUENCE 1047 AA; 118636 MW; 36EC1BB920CDAED2 CRC64;
MGCGLSNETK KTKEPTETKK KDNPDENLYS RQMAVYGAET QGKLMKMKVF IYGLQGVGIE
VAKNLVLAGP SQVVIYDDNI CKSVDQGVNF YIQEKHVKNN STRAEASAEQ LQQLNPYCQV
TILKGEIDTQ VLSSYNVVVF TDYFNKEKLI EFNNFCREKG IGFIYTANLG LYGCAFVDFG
QKHKVFDNNG EDPKHSIVVS ITQDKEGLVT THEDKRHGLV DGDHVTFKEV QGMTEVNDQV
YKVTVKSPFT FTIAQDTSKF KAYQREGIVQ QVKVCEEIQF NSLQQSLNNP IAPGKDCLEM
CDFEKIGRPE QLHIILNGIF EFCKHNNGQL PQLLNQDHSK QLKEIVHKLL ESNKADASNK
FKVEEIPDEL IQNVSLYARA HISPVASFWG GVVAQEIVKF TGKFTPLRQW LHHEVFECLP
DSQVTREVVD SQNGHYVAIF GKEFQESLSK IKLFLVGAGA LGCEYLKMFA LMGMSTGQSG
LVSVTDDDNI ETSNLNRQFL FRKENVGKSK SETACQVAKN MNNRLNVKSY KLRVAPENEQ
FFNDDFWVSL DFVVNAVDNV KARLFVDAQC VWFEKPLFES GTLGTKCNSQ IVIPKLTQSY
GDSADPPEES IPLCTLKNFP HQIEHTIQWA RDYFEGIMVE GPNELSQFIK NPQEYLSKMQ
RENEGKSGIL RAKLEILQKL AIAFNGGTYQ NCVTLSRELF QEMFTNQIAQ LLHSFPLDHK
TEEGQPFWSG PKRPPQIIYF DENDEEHINF IQSSANIFAY LFGLKYNTNR EEIKKMAKSV
HVREFKPGNV KISTNQNDNT QNVAEDDEQI CTKIADELLK LKISSSKKIN TTEFEKDDPT
NYHIDYVSAI ANLRARNYKI TEVDKFKVKL IAGKIIPALA TTTAMVVGAV GLEIIKYILK
KPITQIKNSF MNLALPMWLF SEPLPPMKHK DKDYDEILLG PVKAIPPGFT NWDKIDVVGP
LTVQGLLDYF SQQYQVKLSI ISVAKICIYN SYAGDSERLT QDIAALYEKL NKAPISQFKK
FLEITASGET LNDGVDVNMP IVKYKYK
//
