ID I8YFA9_9BACE Unreviewed; 1075 AA.
AC I8YFA9;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 27-MAR-2024, entry version 54.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU361154};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU361154};
DE AltName: Full=Lactase {ECO:0000256|ARBA:ARBA00032230, ECO:0000256|RuleBase:RU361154};
GN ORFNames=HMPREF1071_02938 {ECO:0000313|EMBL:EIY61067.1};
OS Bacteroides salyersiae CL02T12C01.
OC Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC Bacteroides.
OX NCBI_TaxID=997887 {ECO:0000313|EMBL:EIY61067.1, ECO:0000313|Proteomes:UP000005150};
RN [1] {ECO:0000313|EMBL:EIY61067.1, ECO:0000313|Proteomes:UP000005150}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CL02T12C01 {ECO:0000313|EMBL:EIY61067.1,
RC ECO:0000313|Proteomes:UP000005150};
RG The Broad Institute Genome Sequencing Platform;
RA Earl A., Ward D., Feldgarden M., Gevers D., Zitomersky N.L., Coyne M.J.,
RA Comstock L.E., Young S.K., Zeng Q., Gargeya S., Fitzgerald M., Haas B.,
RA Abouelleil A., Alvarado L., Arachchi H.M., Berlin A., Chapman S.B.,
RA Gearin G., Goldberg J., Griggs A., Gujja S., Hansen M., Heiman D.,
RA Howarth C., Larimer J., Lui A., MacDonald P.J.P., McCowen C.,
RA Montmayeur A., Murphy C., Neiman D., Pearson M., Priest M., Roberts A.,
RA Saif S., Shea T., Sisk P., Stolte C., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The Genome Sequence of Bacteroides salyersiae CL02T12C01.";
RL Submitted (FEB-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|RuleBase:RU361154};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family.
CC {ECO:0000256|ARBA:ARBA00007401, ECO:0000256|RuleBase:RU361154}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EIY61067.1}.
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DR EMBL; AGXV01000035; EIY61067.1; -; Genomic_DNA.
DR RefSeq; WP_007480870.1; NZ_JH724308.1.
DR AlphaFoldDB; I8YFA9; -.
DR PATRIC; fig|997887.3.peg.3039; -.
DR HOGENOM; CLU_002346_0_2_10; -.
DR OrthoDB; 9801077at2; -.
DR Proteomes; UP000005150; Unassembled WGS sequence.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:1901575; P:organic substance catabolic process; IEA:UniProt.
DR Gene3D; 2.70.98.10; -; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR004199; B-gal_small/dom_5.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR014718; GH-type_carb-bd.
DR InterPro; IPR006101; Glyco_hydro_2.
DR InterPro; IPR023232; Glyco_hydro_2_AS.
DR InterPro; IPR006103; Glyco_hydro_2_cat.
DR InterPro; IPR023230; Glyco_hydro_2_CS.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR006104; Glyco_hydro_2_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR032312; LacZ_4.
DR PANTHER; PTHR46323; BETA-GALACTOSIDASE; 1.
DR PANTHER; PTHR46323:SF2; BETA-GALACTOSIDASE; 1.
DR Pfam; PF02929; Bgal_small_N; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF02836; Glyco_hydro_2_C; 1.
DR Pfam; PF02837; Glyco_hydro_2_N; 1.
DR Pfam; PF16353; LacZ_4; 1.
DR PRINTS; PR00132; GLHYDRLASE2.
DR SMART; SM01038; Bgal_small_N; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 2.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR PROSITE; PS00719; GLYCOSYL_HYDROL_F2_1; 1.
DR PROSITE; PS00608; GLYCOSYL_HYDROL_F2_2; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361154};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361154};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..19
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 20..1075
FT /note="Beta-galactosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003717176"
FT DOMAIN 774..1060
FT /note="Beta galactosidase small chain/"
FT /evidence="ECO:0000259|SMART:SM01038"
FT REGION 987..1010
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 996..1010
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1075 AA; 122715 MW; 9D55917742F35DF4 CRC64;
MKNLLTVACL VILPLTSIAQ GERYKQITDP QLTSIRKEAP RSSFTSYTNE KDAAVNNRKD
GTFRLSLNGK WKFNYVDSFS TRPTDFMLPE KNVSDWADIQ VPGNWERQGF GIPIYVNTSY
EFCSPGYAPY WDRPNPPYVP EEWNPTGTYR RDFTLPADWN GKEIFLSADG TKGAAFFYLN
GQFAGMSKDA KTPARFNITQ FVKPGKNVIA VQVHRFSDAN YLECQDFWRL SGFERDVYLY
AQPKLRMADF KVESPLDADY KDGILKLKVK VADSGKPVST SYVLSYKLLD KDGKQVATSD
YAVEAMPVSA PSQSTNEFIF DDNIIRNPKQ WTAETPYLYT LIISLREPEN GEVLEATSVK
VGFRTVEMKN HQLCVNGKPI LVKGVNVHEH NEYTGHYVTE ELMLKDFELW KKYNVNTVRT
CHYPQQERFY ELCDEYGMYV IDEANIESHG MGYNREVGGT LANNPLFMDA HVARTVNMYE
RDKNHPSVIV WSLGNEAGNG VNFYKTYSLL KELDSRPVQY EQAHLEWNTD ICCPMYSRPA
DIEKYAKNPK HTRPLILCEY AHAMGNSLGN FQEYWDIIEK YPLLQGGCIW DWVDQGFAEK
TADGRKYWAY GGDYGATGTP SDGDFCINGV VYPDRSVKPQ TTEMGKVYQN IKFVNFKKEA
GTVDVRNDFS FTNLDKYDYH YIIRDHGKEI YTDKFRASGA PGETVTVQLK GIPQEQTNTG
DVRIEFYATV RTPEPFLPAG TVIAREQNYI YTFHKDNAAL QAAAPVKETE TQAIYSGKNF
KAVFDKKSGM LVSYKYNGME YILNGQGLHP NFWRAPIDND YGAGLPHKLG VWKEVSYGEV
TAAVFNARNG EVTCSYKFPK ADASWDITYK IYADGVIKVN NRFVAANEKT PMIPRIGLRM
QMPETFTTLT YYGRGPEENY RDRRTSQFFG EYSLPIKDMY EPYIRPQENN HRTDIYWCAF
TNKSGAGLLL VADRTFELNA SNHPLETLDS GDDLHNAAPR TAETDHRHLT DPKPEKVVDV
FVDYRMMGIG GDNSWGALPH EPYLIRTGSG NAIEYGFTLV PFGRKVDFRN LIYQY
//
