ID IL13_HUMAN Reviewed; 146 AA.
AC P35225; O43644; Q4VB52; Q9UDC7;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 2.
DT 01-MAY-2013, entry version 143.
DE RecName: Full=Interleukin-13;
DE Short=IL-13;
DE Flags: Precursor;
GN Name=IL13; Synonyms=NC30;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8096327; DOI=10.1038/362248a0;
RA Minty A.J., Chalon P., Derocq J.-M., Dumont X., Guillemot J.-C.,
RA Kaghad M., Labit C., Leplatois P., Liauzun P., Miloux B., Minty C.,
RA Casellas P., Loison G., Lupker J., Shire D., Ferrara P., Caput D.;
RT "Interleukin-13 is a new human lymphokine regulating inflammatory and
RT immune responses.";
RL Nature 362:248-250(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8097324; DOI=10.1073/pnas.90.8.3735;
RA McKenzie A.N., Culpepper J.A., Waal Malefyt R., Briere F.,
RA Punnonen J., Aversa G., Sato A., Dang W., Cocks B.G., Menon S.,
RA de Vries J.E., Banchereau J., Zurawski G.R.;
RT "Interleukin 13, a T-cell-derived cytokine that regulates human
RT monocyte and B-cell function.";
RL Proc. Natl. Acad. Sci. U.S.A. 90:3735-3739(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Blood;
RX PubMed=7721105; DOI=10.1016/0378-1119(94)00720-D;
RA Smirnov D.V., Smirnova M.G., Korobko V.G., Frolova E.I.;
RT "Tandem arrangement of human genes for interleukin-4 and interleukin-
RT 13: resemblance in their organization.";
RL Gene 155:277-281(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8605348;
RA Dolganov G., Bort S., Lovett M., Burr J., Schubert L., Short D.,
RA McGurn M., Gibson C., Lewis D.B.;
RT "Coexpression of the interleukin-13 and interleukin-4 genes correlates
RT with their physical linkage in the cytokine gene cluster on human
RT chromosome 5q23-31.";
RL Blood 87:3316-3326(1996).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT GLN-144.
RG SeattleSNPs variation discovery resource;
RL Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT GLN-144.
RX PubMed=15372022; DOI=10.1038/nature02919;
RA Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S.,
RA Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M.,
RA She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.,
RA Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M.,
RA Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T.,
RA Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M.,
RA Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K.,
RA Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C.,
RA Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M.,
RA Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A.,
RA Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M.,
RA Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M.,
RA Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S.,
RA Richardson P., Lucas S.M., Myers R.M., Rubin E.M.;
RT "The DNA sequence and comparative analysis of human chromosome 5.";
RL Nature 431:268-274(2004).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT GLN-144.
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [8]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 14-146.
RA Jang J.S., Kim B.E.;
RL Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
RN [9]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 15-63.
RC TISSUE=Peripheral blood lymphocyte;
RX PubMed=1408833; DOI=10.1093/nar/20.19.5173;
RA Morgan J.G., Dolganov G.M., Robbins S.E., Hinton L.M., Lovett M.;
RT "The selective isolation of novel cDNAs encoded by the regions
RT surrounding the human interleukin 4 and 5 genes.";
RL Nucleic Acids Res. 20:5173-5179(1992).
RN [10]
RP STRUCTURE BY NMR OF 35-146, AND DISULFIDE BONDS.
RX PubMed=11419948; DOI=10.1006/jmbi.2001.4764;
RA Moy F.J., Diblasio E., Wilhelm J., Powers R.;
RT "Solution structure of human IL-13 and implication for receptor
RT binding.";
RL J. Mol. Biol. 310:219-230(2001).
RN [11]
RP X-RAY CRYSTALLOGRAPHY (3.05 ANGSTROMS) OF 15-146 IN COMPLEX WITH
RP IL13RA2, AND DISULFIDE BONDS.
RX PubMed=20223216; DOI=10.1016/j.str.2010.01.003;
RA Lupardus P.J., Birnbaum M.E., Garcia K.C.;
RT "Molecular basis for shared cytokine recognition revealed in the
RT structure of an unusually high affinity complex between IL-13 and IL-
RT 13Ralpha2.";
RL Structure 18:332-342(2010).
RN [12]
RP VARIANT GLN-144.
RX PubMed=10699178; DOI=10.1093/hmg/9.4.549;
RA Heinzmann A., Mao X.-Q., Akaiwa M., Kreomer R.T., Gao P.-S.,
RA Ohshima K., Umeshita R., Abe Y., Braun S., Yamashita T., Roberts M.H.,
RA Sugimoto R., Arima K., Arinobu Y., Yu B., Kruse S., Enomoto T.,
RA Dake Y., Kawai M., Shimazu S., Sasaki S., Adra C.N., Kitaichi M.,
RA Inoue H., Yamauchi K., Tomichi N., Kurimoto F., Hamasaki N.,
RA Hopkin J.M., Izuhara K., Shirakawa T., Deichmann K.A.;
RT "Genetic variants of IL-13 signalling and human asthma and atopy.";
RL Hum. Mol. Genet. 9:549-559(2000).
RN [13]
RP VARIANT GLN-144.
RX PubMed=11588017;
RA Howard T.D., Whittaker P.A., Zaiman A.L., Koppelman G.H., Xu J.,
RA Hanley M.T., Meyers D.A., Postma D.S., Bleecker E.R.;
RT "Identification and association of polymorphisms in the interleukin-13
RT gene with asthma and atopy in a Dutch population.";
RL Am. J. Respir. Cell Mol. Biol. 25:377-384(2001).
RN [14]
RP VARIANT GLN-144.
RX PubMed=12928861; DOI=10.1007/s00439-003-1001-x;
RA Wang M., Xing Z.-M., Lu C., Ma Y.-X., Yu D.-L., Yan Z., Wang S.-W.,
RA Yu L.-S.;
RT "A common IL-13 Arg130Gln single nucleotide polymorphism among Chinese
RT atopy patients with allergic rhinitis.";
RL Hum. Genet. 113:387-390(2003).
CC -!- FUNCTION: Cytokine. Inhibits inflammatory cytokine production.
CC Synergizes with IL2 in regulating interferon-gamma synthesis. May
CC be critical in regulating inflammatory and immune responses.
CC -!- SUBUNIT: Interacts with IL13RA2.
CC -!- INTERACTION:
CC P78552:IL13RA1; NbExp=4; IntAct=EBI-1647828, EBI-1391535;
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- DISEASE: Allergic rhinitis (ALRH) [MIM:607154]: A common disease
CC with complex inheritance characterized by mucosal inflammation
CC caused by allergen exposure. Note=Disease susceptibility is
CC associated with variations affecting the gene represented in this
CC entry.
CC -!- SIMILARITY: Belongs to the IL-4/IL-13 family.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-15 is the initiator.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA36107.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
CC Sequence=AAA83738.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
CC Sequence=AAB01681.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
CC Sequence=AAC03535.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
CC Sequence=AAK53823.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
CC Sequence=CAA48824.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
CC -!- WEB RESOURCE: Name=Wikipedia; Note=Interleukin-13 entry;
CC URL="http://en.wikipedia.org/wiki/Interleukin_13";
CC -!- WEB RESOURCE: Name=SeattleSNPs;
CC URL="http://pga.gs.washington.edu/data/il13/";
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DR EMBL; X69079; CAA48823.1; -; mRNA.
DR EMBL; X69079; CAA48824.1; ALT_INIT; mRNA.
DR EMBL; L06801; AAA36107.1; ALT_INIT; mRNA.
DR EMBL; U10307; AAA83738.1; ALT_INIT; Genomic_DNA.
DR EMBL; U31120; AAB01681.1; ALT_INIT; Genomic_DNA.
DR EMBL; AF377331; AAK53823.1; ALT_INIT; Genomic_DNA.
DR EMBL; AC004039; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC096139; AAH96139.1; -; mRNA.
DR EMBL; AF043334; AAC03535.1; ALT_INIT; mRNA.
DR IPI; IPI00807607; -.
DR PIR; I38060; A47481.
DR RefSeq; NP_002179.2; NM_002188.2.
DR UniGene; Hs.845; -.
DR PDB; 1GA3; NMR; -; A=35-146.
DR PDB; 1IJZ; NMR; -; A=35-146.
DR PDB; 1IK0; NMR; -; A=35-146.
DR PDB; 1J9U; Model; -; A=38-146.
DR PDB; 3BPO; X-ray; 3.00 A; A=34-146.
DR PDB; 3G6D; X-ray; 3.20 A; A=35-146.
DR PDB; 3ITR; Model; -; A=38-146.
DR PDB; 3ITS; Model; -; A=38-146.
DR PDB; 3L5W; X-ray; 2.00 A; I/J=35-146.
DR PDB; 3L5X; X-ray; 1.90 A; A=35-146.
DR PDB; 3L5Y; X-ray; 2.80 A; A=35-146.
DR PDB; 3LB6; X-ray; 3.05 A; A/B=15-146.
DR PDB; 4I77; X-ray; 1.90 A; Z=35-146.
DR PDBsum; 1GA3; -.
DR PDBsum; 1IJZ; -.
DR PDBsum; 1IK0; -.
DR PDBsum; 1J9U; -.
DR PDBsum; 3BPO; -.
DR PDBsum; 3G6D; -.
DR PDBsum; 3ITR; -.
DR PDBsum; 3ITS; -.
DR PDBsum; 3L5W; -.
DR PDBsum; 3L5X; -.
DR PDBsum; 3L5Y; -.
DR PDBsum; 3LB6; -.
DR PDBsum; 4I77; -.
DR ProteinModelPortal; P35225; -.
DR DIP; DIP-3224N; -.
DR IntAct; P35225; 1.
DR STRING; 9606.ENSP00000304915; -.
DR DMDM; 239938644; -.
DR PRIDE; P35225; -.
DR DNASU; 3596; -.
DR Ensembl; ENST00000304506; ENSP00000304915; ENSG00000169194.
DR GeneID; 3596; -.
DR KEGG; hsa:3596; -.
DR CTD; 3596; -.
DR GeneCards; GC05P131991; -.
DR H-InvDB; HIX0031957; -.
DR HGNC; HGNC:5973; IL13.
DR HPA; HPA042421; -.
DR MIM; 147683; gene.
DR MIM; 607154; phenotype.
DR neXtProt; NX_P35225; -.
DR PharmGKB; PA199; -.
DR eggNOG; NOG42350; -.
DR HOGENOM; HOG000113028; -.
DR HOVERGEN; HBG006140; -.
DR InParanoid; P35225; -.
DR KO; K05435; -.
DR OrthoDB; EOG437RGJ; -.
DR Pathway_Interaction_DB; il12_stat4pathway; IL12 signaling mediated by STAT4.
DR ChiTaRS; IL13; human.
DR EvolutionaryTrace; P35225; -.
DR GenomeRNAi; 3596; -.
DR NextBio; 14053; -.
DR ArrayExpress; P35225; -.
DR Bgee; P35225; -.
DR CleanEx; HS_IL13; -.
DR Genevestigator; P35225; -.
DR GermOnline; ENSG00000169194; Homo sapiens.
DR GO; GO:0005737; C:cytoplasm; IEA:Compara.
DR GO; GO:0009897; C:external side of plasma membrane; IEA:Compara.
DR GO; GO:0005615; C:extracellular space; ISS:UniProtKB.
DR GO; GO:0005125; F:cytokine activity; TAS:ProtInc.
DR GO; GO:0007267; P:cell-cell signaling; IC:UniProtKB.
DR GO; GO:0006928; P:cellular component movement; TAS:ProtInc.
DR GO; GO:0071345; P:cellular response to cytokine stimulus; IEA:Compara.
DR GO; GO:0071260; P:cellular response to mechanical stimulus; IEA:Compara.
DR GO; GO:0006955; P:immune response; IEA:InterPro.
DR GO; GO:0006954; P:inflammatory response; TAS:ProtInc.
DR GO; GO:1901247; P:negative regulation of lung ciliated cell differentiation; NAS:BHF-UCL.
DR GO; GO:0071635; P:negative regulation of transforming growth factor beta production; IEA:Compara.
DR GO; GO:0030890; P:positive regulation of B cell proliferation; IEA:Compara.
DR GO; GO:0032723; P:positive regulation of connective tissue growth factor production; IEA:Compara.
DR GO; GO:0002639; P:positive regulation of immunoglobulin production; IEA:Compara.
DR GO; GO:1901251; P:positive regulation of lung goblet cell differentiation; NAS:BHF-UCL.
DR GO; GO:0043032; P:positive regulation of macrophage activation; IEA:Compara.
DR GO; GO:2000231; P:positive regulation of pancreatic stellate cell proliferation; IEA:Compara.
DR GO; GO:0050714; P:positive regulation of protein secretion; IEA:Compara.
DR GO; GO:0051281; P:positive regulation of release of sequestered calcium ion into cytosol; IEA:Compara.
DR GO; GO:0048661; P:positive regulation of smooth muscle cell proliferation; IEA:Compara.
DR GO; GO:0042526; P:positive regulation of tyrosine phosphorylation of Stat6 protein; IEA:Compara.
DR GO; GO:0010155; P:regulation of proton transport; IEA:Compara.
DR GO; GO:0045471; P:response to ethanol; IEA:Compara.
DR GO; GO:0032496; P:response to lipopolysaccharide; IEA:Compara.
DR GO; GO:0035094; P:response to nicotine; IEA:Compara.
DR GO; GO:0007165; P:signal transduction; NAS:ProtInc.
DR Gene3D; 1.20.1250.10; -; 1.
DR InterPro; IPR009079; 4_helix_cytokine-like_core.
DR InterPro; IPR012351; 4_helix_cytokine_core.
DR InterPro; IPR020470; Interleukin-13.
DR InterPro; IPR018096; Interleukin-4/-13_CS.
DR InterPro; IPR001325; Interleukin-4/13.
DR PANTHER; PTHR11322; PTHR11322; 1.
DR Pfam; PF03487; IL13; 1.
DR PRINTS; PR01929; INTRLEUKIN13.
DR SMART; SM00190; IL4_13; 1.
DR SUPFAM; SSF47266; 4_helix_cytokine; 1.
DR PROSITE; PS00838; INTERLEUKIN_4_13; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Complete proteome; Cytokine; Disulfide bond;
KW Glycoprotein; Polymorphism; Reference proteome; Secreted; Signal.
FT SIGNAL 1 24 Potential.
FT CHAIN 25 146 Interleukin-13.
FT /FTId=PRO_0000015547.
FT CARBOHYD 52 52 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 63 63 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 71 71 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 86 86 N-linked (GlcNAc...) (Potential).
FT DISULFID 62 90
FT DISULFID 78 104
FT VARIANT 144 144 R -> Q (associated with increased risk
FT for asthma development; at homozygosity
FT associated with higher levels of serum
FT total IgE in some allergic rhinitis
FT patients; dbSNP:rs20541).
FT /FTId=VAR_010037.
FT CONFLICT 1 1 M -> R (in Ref. 2; CAA48824).
FT CONFLICT 26 26 T -> A (in Ref. 9; no nucleotide entry).
FT CONFLICT 32 32 A -> D (in Ref. 9; no nucleotide entry).
FT CONFLICT 51 51 V -> S (in Ref. 9; no nucleotide entry).
FT CONFLICT 56 56 N -> T (in Ref. 9; no nucleotide entry).
FT CONFLICT 59 59 A -> R (in Ref. 3; AAA83738).
FT CONFLICT 101 101 S -> G (in Ref. 8; AAC03535).
FT CONFLICT 112 112 Missing (in Ref. 3; AAA83738).
FT HELIX 41 54
FT TURN 61 64
FT STRAND 66 68
FT TURN 74 76
FT HELIX 77 84
FT HELIX 91 93
FT HELIX 94 103
FT STRAND 104 106
FT STRAND 122 124
FT HELIX 125 142
SQ SEQUENCE 146 AA; 15816 MW; BE2D46CCF913D129 CRC64;
MHPLLNPLLL ALGLMALLLT TVIALTCLGG FASPGPVPPS TALRELIEEL VNITQNQKAP
LCNGSMVWSI NLTAGMYCAA LESLINVSGC SAIEKTQRML SGFCPHKVSA GQFSSLHVRD
TKIEVAQFVK DLLLHLKKLF REGRFN
//
