ID INSL3_DROME Reviewed; 120 AA.
AC Q9VT52;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2004, sequence version 2.
DT 27-MAR-2024, entry version 152.
DE RecName: Full=Probable insulin-like peptide 3;
DE Short=dILP3;
DE AltName: Full=Insulin-related peptide 3;
DE Contains:
DE RecName: Full=Probable insulin-like peptide 3 A chain;
DE Contains:
DE RecName: Full=Probable insulin-like peptide 3 B chain;
DE Flags: Precursor;
GN Name=Ilp3; ORFNames=CG14167;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP IDENTIFICATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=11250149; DOI=10.1016/s0960-9822(01)00068-9;
RA Brogiolo W., Stocker H., Ikeya T., Rintelen F., Fernandez R., Hafen E.;
RT "An evolutionarily conserved function of the Drosophila insulin receptor
RT and insulin-like peptides in growth control.";
RL Curr. Biol. 11:213-221(2001).
CC -!- SUBUNIT: Heterodimer of a B chain and an A chain linked by two
CC disulfide bonds. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed at a high level in seven cells of each
CC larval brain hemisphere that may correspond to neurosecretory cells.
CC {ECO:0000269|PubMed:11250149}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the larva, but not in the embryo.
CC {ECO:0000269|PubMed:11250149}.
CC -!- SIMILARITY: Belongs to the insulin family. {ECO:0000305}.
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DR EMBL; AE014296; AAF50203.2; -; Genomic_DNA.
DR RefSeq; NP_648360.2; NM_140103.3.
DR AlphaFoldDB; Q9VT52; -.
DR BioGRID; 64539; 2.
DR STRING; 7227.FBpp0076102; -.
DR PaxDb; 7227-FBpp0076102; -.
DR DNASU; 39151; -.
DR EnsemblMetazoa; FBtr0076373; FBpp0076102; FBgn0044050.
DR GeneID; 39151; -.
DR KEGG; dme:Dmel_CG14167; -.
DR AGR; FB:FBgn0044050; -.
DR CTD; 39151; -.
DR FlyBase; FBgn0044050; Ilp3.
DR VEuPathDB; VectorBase:FBgn0044050; -.
DR eggNOG; ENOG502S3FQ; Eukaryota.
DR HOGENOM; CLU_125164_0_1_1; -.
DR InParanoid; Q9VT52; -.
DR OMA; VYGFNAM; -.
DR OrthoDB; 3028803at2759; -.
DR PhylomeDB; Q9VT52; -.
DR Reactome; R-DME-110478; Insulin signaling pathway.
DR SignaLink; Q9VT52; -.
DR BioGRID-ORCS; 39151; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 39151; -.
DR PRO; PR:Q9VT52; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0044050; Expressed in brain and 9 other cell types or tissues.
DR ExpressionAtlas; Q9VT52; baseline and differential.
DR Genevisible; Q9VT52; DM.
DR GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR GO; GO:0005615; C:extracellular space; IDA:FlyBase.
DR GO; GO:0005179; F:hormone activity; IEA:InterPro.
DR GO; GO:0005158; F:insulin receptor binding; ISS:UniProtKB.
DR GO; GO:0060180; P:female mating behavior; IMP:FlyBase.
DR GO; GO:0008286; P:insulin receptor signaling pathway; IMP:FlyBase.
DR GO; GO:0045475; P:locomotor rhythm; IMP:FlyBase.
DR GO; GO:0061964; P:negative regulation of entry into reproductive diapause; IMP:FlyBase.
DR GO; GO:0009743; P:response to carbohydrate; IEP:FlyBase.
DR GO; GO:0030431; P:sleep; IMP:FlyBase.
DR CDD; cd04366; IlGF_insulin_bombyxin_like; 1.
DR Gene3D; 1.10.100.10; Insulin-like; 1.
DR InterPro; IPR016179; Insulin-like.
DR InterPro; IPR036438; Insulin-like_sf.
DR InterPro; IPR040228; Insulin-relat-peptide_inver.
DR InterPro; IPR022353; Insulin_CS.
DR InterPro; IPR022352; Insulin_family.
DR PANTHER; PTHR13647; INSULIN-LIKE PEPTIDE 2-RELATED; 1.
DR PANTHER; PTHR13647:SF6; INSULIN-LIKE PEPTIDE 2-RELATED; 1.
DR Pfam; PF00049; Insulin; 1.
DR PRINTS; PR00276; INSULINFAMLY.
DR SMART; SM00078; IlGF; 1.
DR SUPFAM; SSF56994; Insulin-like; 1.
DR PROSITE; PS00262; INSULIN; 1.
PE 2: Evidence at transcript level;
KW Cleavage on pair of basic residues; Disulfide bond; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..29
FT /evidence="ECO:0000255"
FT CHAIN 30..120
FT /note="Probable insulin-like peptide 3"
FT /id="PRO_0000016193"
FT PEPTIDE 30..48
FT /note="Probable insulin-like peptide 3 B chain"
FT /evidence="ECO:0000255"
FT /id="PRO_0000016194"
FT PROPEP 51..89
FT /note="Connecting peptide"
FT /evidence="ECO:0000255"
FT /id="PRO_0000016195"
FT PEPTIDE 92..120
FT /note="Probable insulin-like peptide 3 A chain"
FT /evidence="ECO:0000255"
FT /id="PRO_0000016196"
FT DISULFID 34..101
FT /note="Interchain (between B and A chains)"
FT /evidence="ECO:0000250"
FT DISULFID 46..114
FT /note="Interchain (between B and A chains)"
FT /evidence="ECO:0000250"
FT DISULFID 100..105
FT /evidence="ECO:0000250"
SQ SEQUENCE 120 AA; 13708 MW; FA834C8BAE1CE31B CRC64;
MGIEMRCQDR RILLPSLLLL ILMIGGVQAT MKLCGRKLPE TLSKLCVYGF NAMTKRTLDP
VNFNQIDGFE DRSLLERLLS DSSVQMLKTR RLRDGVFDEC CLKSCTMDEV LRYCAAKPRT
//
