ID J3A6F5_9PSED Unreviewed; 865 AA.
AC J3A6F5;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 24-JAN-2024, entry version 38.
DE RecName: Full=aconitate hydratase {ECO:0000256|ARBA:ARBA00012926};
DE EC=4.2.1.3 {ECO:0000256|ARBA:ARBA00012926};
GN ORFNames=PMI30_05422 {ECO:0000313|EMBL:EJM60833.1};
OS Pseudomonas sp. GM50.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=1144332 {ECO:0000313|EMBL:EJM60833.1, ECO:0000313|Proteomes:UP000007513};
RN [1] {ECO:0000313|EMBL:EJM60833.1, ECO:0000313|Proteomes:UP000007513}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GM50 {ECO:0000313|EMBL:EJM60833.1,
RC ECO:0000313|Proteomes:UP000007513};
RX PubMed=23045501; DOI=10.1128/JB.01243-12;
RA Brown S.D., Utturkar S.M., Klingeman D.M., Johnson C.M., Martin S.L.,
RA Land M.L., Lu T.Y., Schadt C.W., Doktycz M.J., Pelletier D.A.;
RT "Twenty-one genome sequences from Pseudomonas species and 19 genome
RT sequences from diverse bacteria isolated from the rhizosphere and
RT endosphere of Populus deltoides.";
RL J. Bacteriol. 194:5991-5993(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000256|ARBA:ARBA00007185}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EJM60833.1}.
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DR EMBL; AKJK01000117; EJM60833.1; -; Genomic_DNA.
DR RefSeq; WP_008014110.1; NZ_AKJK01000117.1.
DR AlphaFoldDB; J3A6F5; -.
DR PATRIC; fig|1144332.3.peg.5228; -.
DR Proteomes; UP000007513; Unassembled WGS sequence.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0019679; P:propionate metabolic process, methylcitrate cycle; IEA:InterPro.
DR Gene3D; 6.10.190.10; -; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR012708; 2Me_IsoCit_deHydtase_FeS-dep.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR006249; Aconitase/IRP2.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR NCBIfam; TIGR02333; 2met_isocit_dHY; 1.
DR PANTHER; PTHR11670:SF43; 2-METHYLCITRATE DEHYDRATASE (2-METHYL-TRANS-ACONITATE FORMING); 1.
DR PANTHER; PTHR11670; ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723}.
FT DOMAIN 66..533
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 657..787
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 865 AA; 94671 MW; 123EFFBE97B59059 CRC64;
MNTEFRKPLP GSHLDYFDVR AAVEAIQPGA YDALPYTSRV LAENLVRRCD PATLTESLKQ
FIERKRDLDF PWFPARVVCH DILGQTALVD LAGLRDAIAL QGGDPAQVNP VVPTQLIVDH
SLAVERGGFD PEAFEKNRAI EDRRNEDRFH FINWTKKAFK NVDVIPPGNG IMHQINLEKM
SPVIQVRDGV AFPDTCVGTD SHTPHVDALG VIAIGVGGLE AESVMLGRAS WMRLPESVGV
ELTGKLQPGI TATDMVLALT EYLRKQKVVG AWLEFFGEGA SALTLGDRAT ISNMAPEYGA
TAAMFYIDQQ TIDYLKLTGR EDEQVQLVEN YAKQTGLWAD SLKGAQYERG LSFDLSSVVR
NMAGPSNPHA RVATSDLAAQ GISGQWDDVP GQMPDGAVII AAITSCTNTS NPRNVIAAGL
LARNANKLGL TRKPWVKSSL APGSKTVALY LDEAGLTKEL EQLGFGVVAF ACTTCNGMSG
ALDPVIQQEI IDRDLYATAV LSGNRNFDGR IHPYAKNAFL ASPPLVVAYA IAGTIRFDIE
KDVLGLDANG KEIRLKDIWP SDEEIDAVVK ASVKPEQFRQ VYIPMFAIHE DTGPKVTPLY
DWREMSTYIR RPPYWEGALA GARPLKGMRP LAVLPDNITT DHLSPSNAIM LDSAAGEYLA
KMGLPEEDFN SYATHRGDHL TAQRATFANP KLFNEMVREN GKVKQGSLAR VEPEGKVMRM
WEAIETYMER KQPLIIIAGA DYGQGSSRDW AAKGVRLAGV EAIAAEGFER IHRTNLVGMG
VLPLEFQPGT DRHTLAIDGS ETYDVIGDRT PRATLTLVIN RKNGERVEVP VTCRLDTAEE
VSIYEAGGVL QRFAQDFLEE SAVAV
//
