ID J3AEX1_9SPHN Unreviewed; 989 AA.
AC J3AEX1;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 24-JAN-2024, entry version 49.
DE SubName: Full=Sarcosine oxidase, alpha subunit family, heterotetrameric form {ECO:0000313|EMBL:EJL30871.1};
GN ORFNames=PMI02_01937 {ECO:0000313|EMBL:EJL30871.1};
OS Novosphingobium sp. AP12.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Novosphingobium.
OX NCBI_TaxID=1144305 {ECO:0000313|EMBL:EJL30871.1, ECO:0000313|Proteomes:UP000007515};
RN [1] {ECO:0000313|EMBL:EJL30871.1, ECO:0000313|Proteomes:UP000007515}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AP12 {ECO:0000313|EMBL:EJL30871.1,
RC ECO:0000313|Proteomes:UP000007515};
RX PubMed=23045501; DOI=10.1128/JB.01243-12;
RA Brown S.D., Utturkar S.M., Klingeman D.M., Johnson C.M., Martin S.L.,
RA Land M.L., Lu T.Y., Schadt C.W., Doktycz M.J., Pelletier D.A.;
RT "Twenty-one genome sequences from Pseudomonas species and 19 genome
RT sequences from diverse bacteria isolated from the rhizosphere and
RT endosphere of Populus deltoides.";
RL J. Bacteriol. 194:5991-5993(2012).
CC -!- SIMILARITY: Belongs to the GcvT family.
CC {ECO:0000256|ARBA:ARBA00008609}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EJL30871.1}.
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DR EMBL; AKKE01000049; EJL30871.1; -; Genomic_DNA.
DR RefSeq; WP_007681857.1; NZ_AKKE01000049.1.
DR AlphaFoldDB; J3AEX1; -.
DR STRING; 1144305.PMI02_01937; -.
DR PATRIC; fig|1144305.3.peg.1866; -.
DR eggNOG; COG0404; Bacteria.
DR eggNOG; COG0446; Bacteria.
DR OrthoDB; 5287468at2; -.
DR Proteomes; UP000007515; Unassembled WGS sequence.
DR GO; GO:0008115; F:sarcosine oxidase activity; IEA:InterPro.
DR GO; GO:0046653; P:tetrahydrofolate metabolic process; IEA:InterPro.
DR Gene3D; 3.10.20.440; 2Fe-2S iron-sulphur cluster binding domain, sarcosine oxidase, alpha subunit, N-terminal domain; 1.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR InterPro; IPR042204; 2Fe-2S-bd_N.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR023753; FAD/NAD-binding_dom.
DR InterPro; IPR028896; GCST/YgfZ/DmdA.
DR InterPro; IPR013977; GCV_T_C.
DR InterPro; IPR006222; GCV_T_N.
DR InterPro; IPR029043; GcvT/YgfZ_C.
DR InterPro; IPR006277; Sarcosine_oxidase_asu.
DR InterPro; IPR041117; SoxA_A3.
DR InterPro; IPR027266; TrmE/GcvT_dom1.
DR NCBIfam; TIGR01372; soxA; 1.
DR PANTHER; PTHR43757; AMINOMETHYLTRANSFERASE; 1.
DR PANTHER; PTHR43757:SF2; AMINOMETHYLTRANSFERASE, MITOCHONDRIAL; 1.
DR Pfam; PF13510; Fer2_4; 1.
DR Pfam; PF01571; GCV_T; 1.
DR Pfam; PF08669; GCV_T_C; 1.
DR Pfam; PF07992; Pyr_redox_2; 1.
DR Pfam; PF17806; SO_alpha_A3; 1.
DR PIRSF; PIRSF037980; SoxA; 1.
DR PRINTS; PR00368; FADPNR.
DR PRINTS; PR00469; PNDRDTASEII.
DR SUPFAM; SSF101790; Aminomethyltransferase beta-barrel domain; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR SUPFAM; SSF103025; Folate-binding domain; 1.
PE 3: Inferred from homology;
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000007515}.
FT DOMAIN 173..419
FT /note="FAD/NAD(P)-binding"
FT /evidence="ECO:0000259|Pfam:PF07992"
FT DOMAIN 506..589
FT /note="SoxA A3"
FT /evidence="ECO:0000259|Pfam:PF17806"
FT DOMAIN 605..869
FT /note="Aminomethyltransferase folate-binding"
FT /evidence="ECO:0000259|Pfam:PF01571"
FT DOMAIN 895..981
FT /note="Glycine cleavage T-protein C-terminal barrel"
FT /evidence="ECO:0000259|Pfam:PF08669"
SQ SEQUENCE 989 AA; 105681 MW; 39ED112B5725EDED CRC64;
MSRTDVNRLT NGGLIDRTKP LRFTFDGKNY RGLAGDTLAS ALLANDVRLV GRSFKYHRPR
GILAAGSEEP NALVTLRSGA HAEPNTRATT VPLYDGLEAT SQNRWPSLAF DFLAVNQLAS
PLLVAGFYYK TFMWPAALWE KLYEPLIRRA AGLGKLSMLP DPDHYDREHG FCDLLVIGGG
AAGLAAALTA ARSGARVIVA DEDVMPGGRL LSERHQIDGI LASDWAARVA AELASLPNVR
LLTRTTVFGV YDGREYGAVE RVSDHLSAPS SGQPRQRLWK IIAKRAVLAA GAIERPIVFG
ANDRPGVMMA SAISTYVNRY AVAPGKCAVV FTATDSGWTT AHDLVRAGVG VGAVVDARPA
DARHIELARR LGVEALFGAV VTEVHGRVAK AVTVRTAEGA PRRISCDLVA MAGGWNPAIG
LGSNLGARPI WSDAQQAFLL ESCPPGLAPA GAAAGDLALG DALRGGVHQA SAALADLGIA
PKGLPSFSAS DDFAAATPLW QVPGKAKAFV DFQHDVTVTD VQLAAREGFI SIEHLKRYTT
LGMATDQGKT SQLNGHAVLA AVIGKTIAET GTVLSRPPYL PVAIGALAGH HRGQDFRPMR
KTASHRWAQA QRAIFTDAGQ WKRAQWFARS GESEWLESVN REVLATRGSV GVCDVSTLGK
IDVQGPDAGT LLDRLYINNF ASLPIGKARY GVMLREDGFV MDDGTTTRFA EDSYFVTTTT
VNAAKVMQHI DYARQVLWPE LDVQATSVTE QWATYAVAGP KSRALLQALL PSVDLANDAL
PYMGAIELVW KERVCRLFRL SFSGELAYEI SVPARLGEAL ILALFEAGRA FDVTAYGTEA
LSVMRIEKGH PAGNELNGTT TAADLGMGRM MSTKKDYLGR VMAGRPGLCD PERPRLVGLK
PVNGRSLIRA GAHLLSPGQD AVAANDEGFV SSAAYSPTLG LPIALAFLKL GHQRHGERII
VHDPVRGADV EAAICNPVFF DPEGVRVRG
//
