ID J3G998_9PSED Unreviewed; 972 AA.
AC J3G998;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE SubName: Full=NADH:ubiquinone oxidoreductase subunit 5 (Chain L)/multisubunit Na+/H+ antiporter, MnhA subunit {ECO:0000313|EMBL:EJM58005.1};
GN ORFNames=PMI28_02527 {ECO:0000313|EMBL:EJM58005.1};
OS Pseudomonas sp. GM48.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=1144330 {ECO:0000313|EMBL:EJM58005.1, ECO:0000313|Proteomes:UP000007293};
RN [1] {ECO:0000313|EMBL:EJM58005.1, ECO:0000313|Proteomes:UP000007293}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GM48 {ECO:0000313|EMBL:EJM58005.1,
RC ECO:0000313|Proteomes:UP000007293};
RX PubMed=23045501; DOI=10.1128/JB.01243-12;
RA Brown S.D., Utturkar S.M., Klingeman D.M., Johnson C.M., Martin S.L.,
RA Land M.L., Lu T.Y., Schadt C.W., Doktycz M.J., Pelletier D.A.;
RT "Twenty-one genome sequences from Pseudomonas species and 19 genome
RT sequences from diverse bacteria isolated from the rhizosphere and
RT endosphere of Populus deltoides.";
RL J. Bacteriol. 194:5991-5993(2012).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004651};
CC Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004651}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141, ECO:0000256|RuleBase:RU000320}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141,
CC ECO:0000256|RuleBase:RU000320}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EJM58005.1}.
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DR EMBL; AKJM01000066; EJM58005.1; -; Genomic_DNA.
DR RefSeq; WP_007986257.1; NZ_AKJM01000066.1.
DR AlphaFoldDB; J3G998; -.
DR PATRIC; fig|1144330.3.peg.2490; -.
DR OrthoDB; 9811798at2; -.
DR Proteomes; UP000007293; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006811; P:monoatomic ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR007182; MnhB.
DR InterPro; IPR025383; MrpA_C/MbhD.
DR InterPro; IPR046806; MrpA_C/MbhE.
DR InterPro; IPR001750; ND/Mrp_mem.
DR InterPro; IPR001516; Proton_antipo_N.
DR PANTHER; PTHR43373:SF1; ANTIPORTER SUBUNIT MNHA2-RELATED; 1.
DR PANTHER; PTHR43373; NA(+)/H(+) ANTIPORTER SUBUNIT; 1.
DR Pfam; PF13244; MbhD; 1.
DR Pfam; PF20501; MbhE; 1.
DR Pfam; PF04039; MnhB; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR Pfam; PF00662; Proton_antipo_N; 1.
DR PRINTS; PR01434; NADHDHGNASE5.
PE 4: Predicted;
KW Antiport {ECO:0000256|ARBA:ARBA00022449};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Ion transport {ECO:0000256|ARBA:ARBA00023065};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Signal {ECO:0000256|SAM:SignalP};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU000320};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448};
KW Ubiquinone {ECO:0000313|EMBL:EJM58005.1}.
FT SIGNAL 1..17
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 18..972
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003768776"
FT TRANSMEM 27..47
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 78..96
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 108..126
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 132..150
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 162..185
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 205..230
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 242..264
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 270..291
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 298..316
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 367..389
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 459..482
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 502..523
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 570..590
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 602..624
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 631..651
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 657..678
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 703..721
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 759..777
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 814..841
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 847..867
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 888..907
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 927..949
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 64..110
FT /note="NADH-Ubiquinone oxidoreductase (complex I) chain 5
FT N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00662"
FT DOMAIN 126..399
FT /note="NADH:quinone oxidoreductase/Mrp antiporter membrane
FT subunit"
FT /evidence="ECO:0000259|Pfam:PF00361"
FT DOMAIN 615..680
FT /note="MrpA C-terminal/MbhD"
FT /evidence="ECO:0000259|Pfam:PF13244"
FT DOMAIN 697..794
FT /note="MrpA C-terminal/MbhE"
FT /evidence="ECO:0000259|Pfam:PF20501"
FT DOMAIN 821..943
FT /note="Na+/H+ antiporter MnhB subunit-related protein"
FT /evidence="ECO:0000259|Pfam:PF04039"
SQ SEQUENCE 972 AA; 105729 MW; DA5EDCD3991F8CFE CRC64;
MSLIVLLLLP FIGSCLAAVL PHNARNAESL LAGLVALIGT VQVALLYPQI AHGGVIREEF
FWLPSLGLNF VLRMDGFAWL FSMLVLGIGT LVSLYARYYM SPDDPVPRFF AFFLAFMGAM
LGLVVSGNLI QIVFFWELTS LFSFLLIGYW HHRADARRGA YMALMVTGAG GLCLLAGVML
LGHVVGSYDL DKVLAAGDLI RAHALYPILL PLILIGALSK SAQFPFHFWL PHAMAAPTPV
SAYLHSATMV KAGVFLLARL WPSLSGSEEW FYIVSGAGAC TLLLGAYCAM FQNDLKGLLA
YSTISHLGLI TLLLGLNSPL AAVAAVFHIL NHATFKASLF MAAGIIDHES GTRDIRKLSG
LFKLMPFTAT LAMVASASMA GVPLLNGFLS KEMFFAETVF INATRWVELS LPIVATIAGT
FSVAYSLRFT VDVFFGPPAT DLPHTPHEPP RWMRAPVELL VFTCLLVGIF PAQVVGPLLA
AAAQPVVGGT LPEYSLAIWH GLNAPMIMSL IALSCGTLLY LLLRNQFKRG RFHYPPIIGR
LDGKRLFERG LVVMMRLARR LEPRIGTRRL QMQLFLVVLA AVLAGLIPML HSSLHWGDRP
KIPGSIVFVT LWLLAIACAL GAAYQAKYHR LAALTMVSVC GLMTCVTFVW FSAPDLALTQ
LAVEVVTTVL ILLGLRWLPR RIEEVSPLPG SLRRARVRRI RDLLLSTTVG IGMALLAYAM
LTRQTPNDIS SFYLSRALPE GGGSNVVNVM LVDFRGFDTL GEITVLVAVA LTVFALLRRF
RPPKESLQLP AQQRLLAPDV VTDLVNPRHA SDTALGFMMV PAVLVRLLLP VALVVSFYLF
MRGHNQPGGG FVAGLVMSVA FILQYMVAGT QWVEAQMSLR PLRWMGTGLL FATATGLGAM
VVGYPFLTTH TWHFSLPLLG DIHLASALFF DIGVYGVVVG STLLILTALA HQSVRGHKTA
AQPKSVAIKE VV
//
