ID J3GDF4_9PSED Unreviewed; 548 AA.
AC J3GDF4;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 24-JAN-2024, entry version 51.
DE RecName: Full=Probable malate:quinone oxidoreductase {ECO:0000256|HAMAP-Rule:MF_00212};
DE EC=1.1.5.4 {ECO:0000256|HAMAP-Rule:MF_00212};
DE AltName: Full=MQO {ECO:0000256|HAMAP-Rule:MF_00212};
DE AltName: Full=Malate dehydrogenase [quinone] {ECO:0000256|HAMAP-Rule:MF_00212};
GN Name=mqo {ECO:0000256|HAMAP-Rule:MF_00212};
GN ORFNames=PMI30_05160 {ECO:0000313|EMBL:EJM61570.1};
OS Pseudomonas sp. GM50.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=1144332 {ECO:0000313|EMBL:EJM61570.1, ECO:0000313|Proteomes:UP000007513};
RN [1] {ECO:0000313|EMBL:EJM61570.1, ECO:0000313|Proteomes:UP000007513}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GM50 {ECO:0000313|EMBL:EJM61570.1,
RC ECO:0000313|Proteomes:UP000007513};
RX PubMed=23045501; DOI=10.1128/JB.01243-12;
RA Brown S.D., Utturkar S.M., Klingeman D.M., Johnson C.M., Martin S.L.,
RA Land M.L., Lu T.Y., Schadt C.W., Doktycz M.J., Pelletier D.A.;
RT "Twenty-one genome sequences from Pseudomonas species and 19 genome
RT sequences from diverse bacteria isolated from the rhizosphere and
RT endosphere of Populus deltoides.";
RL J. Bacteriol. 194:5991-5993(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-malate + a quinone = a quinol + oxaloacetate;
CC Xref=Rhea:RHEA:46012, ChEBI:CHEBI:15589, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:24646, ChEBI:CHEBI:132124; EC=1.1.5.4;
CC Evidence={ECO:0000256|ARBA:ARBA00001139, ECO:0000256|HAMAP-
CC Rule:MF_00212};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974,
CC ECO:0000256|HAMAP-Rule:MF_00212};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle;
CC oxaloacetate from (S)-malate (quinone route): step 1/1.
CC {ECO:0000256|ARBA:ARBA00005012, ECO:0000256|HAMAP-Rule:MF_00212}.
CC -!- SIMILARITY: Belongs to the MQO family. {ECO:0000256|HAMAP-
CC Rule:MF_00212}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EJM61570.1}.
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DR EMBL; AKJK01000108; EJM61570.1; -; Genomic_DNA.
DR RefSeq; WP_008013650.1; NZ_AKJK01000108.1.
DR AlphaFoldDB; J3GDF4; -.
DR PATRIC; fig|1144332.3.peg.4973; -.
DR UniPathway; UPA00223; UER01008.
DR Proteomes; UP000007513; Unassembled WGS sequence.
DR GO; GO:0052589; F:malate dehydrogenase (menaquinone) activity; IEA:UniProtKB-EC.
DR GO; GO:0008924; F:malate dehydrogenase (quinone) activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00212; MQO; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR006231; MQO.
DR NCBIfam; TIGR01320; mal_quin_oxido; 1.
DR PANTHER; PTHR43104; L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR43104:SF2; L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL; 1.
DR Pfam; PF06039; Mqo; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|HAMAP-Rule:MF_00212};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630, ECO:0000256|HAMAP-
KW Rule:MF_00212};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002, ECO:0000256|HAMAP-
KW Rule:MF_00212}; Signal {ECO:0000256|SAM:SignalP};
KW Tricarboxylic acid cycle {ECO:0000256|HAMAP-Rule:MF_00212}.
FT SIGNAL 1..23
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 24..548
FT /note="Probable malate:quinone oxidoreductase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003767653"
SQ SEQUENCE 548 AA; 59333 MW; 17349F774757D964 CRC64;
MFKKLNTALL GLALSMGITS AYAEEAKKVD VLLIGGGIMS ATLGVWLNEL EPGLSMEMVE
RLDGVAQESS NGWNNAGTGH SALAELNYTP EDDKGNVQIA KAVEINEAFQ ISRQFWAWQV
QQGVLKNPRS FINSTPHMSF VWGDDNIKFL KKRYEALQAS PLFAGMQYSE DPAVIKKWVP
LMMEGRDPNQ KVAATWSPIG TDMNFGEITR QFVAHLQTTP KFDLKLSSEV QDITKNADGS
WRVSYKNLKD GTKTETDAKF VFIGAGGGAL HLLQKSGIPE AQEYAGFPVG GSFLVTENPT
IAEQHLAKAY GKASVGAPPM SVPHLDTRVL DGKRVILFGP FATFSTKFLK EGSYLDLLTT
TTTHNVWPMT KVGIKEYPLV EYLAGQLMLS DEDRLNALKE YFPNAKAEDW RLWQAGQRVQ
IIKRDEAAGG VLKLGTEIVA SADGTIAGLL GASPGASTAA PIMLTVLQKV FKDKVATPAW
QEKLHQIVPS YGTQLNSNPA KVAEEWAYTA KVLELTPPPV IGQAAAPAAA PAAPAKAPKA
NAATDMAL
//
