ID   J3H6P6_9PSED            Unreviewed;       720 AA.
AC   J3H6P6;
DT   03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT   03-OCT-2012, sequence version 1.
DT   24-JAN-2024, entry version 47.
DE   SubName: Full=Glycogen debranching enzyme GlgX {ECO:0000313|EMBL:EJM86112.1};
GN   ORFNames=PMI34_04009 {ECO:0000313|EMBL:EJM86112.1};
OS   Pseudomonas sp. GM74.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=1144336 {ECO:0000313|EMBL:EJM86112.1, ECO:0000313|Proteomes:UP000007290};
RN   [1] {ECO:0000313|EMBL:EJM86112.1, ECO:0000313|Proteomes:UP000007290}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GM74 {ECO:0000313|EMBL:EJM86112.1,
RC   ECO:0000313|Proteomes:UP000007290};
RX   PubMed=23045501; DOI=10.1128/JB.01243-12;
RA   Brown S.D., Utturkar S.M., Klingeman D.M., Johnson C.M., Martin S.L.,
RA   Land M.L., Lu T.Y., Schadt C.W., Doktycz M.J., Pelletier D.A.;
RT   "Twenty-one genome sequences from Pseudomonas species and 19 genome
RT   sequences from diverse bacteria isolated from the rhizosphere and
RT   endosphere of Populus deltoides.";
RL   J. Bacteriol. 194:5991-5993(2012).
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC       {ECO:0000256|ARBA:ARBA00008061}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:EJM86112.1}.
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DR   EMBL; AKJG01000106; EJM86112.1; -; Genomic_DNA.
DR   RefSeq; WP_008052173.1; NZ_AKJG01000106.1.
DR   AlphaFoldDB; J3H6P6; -.
DR   PATRIC; fig|1144336.3.peg.3913; -.
DR   Proteomes; UP000007290; Unassembled WGS sequence.
DR   GO; GO:0004133; F:glycogen debranching enzyme activity; IEA:InterPro.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR   CDD; cd11326; AmyAc_Glg_debranch; 1.
DR   CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR011837; Glycogen_debranch_GlgX.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   InterPro; IPR048644; Isoamylase_C.
DR   NCBIfam; TIGR02100; glgX_debranch; 1.
DR   PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR   PANTHER; PTHR43002:SF9; GLYCOSYL HYDROLASE-RELATED; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF02922; CBM_48; 1.
DR   Pfam; PF21331; Isoamylase_C; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF81296; E set domains; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   3: Inferred from homology;
FT   DOMAIN          179..585
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
FT   REGION          480..507
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        489..503
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   720 AA;  81645 MW;  BCB0790A3BD1CFE8 CRC64;
     MTSPKKAAAP APRVEATRIR EGLPFPLGAT WDGLGVNFAL FSAHATKVEL CLFDDAGEVE
     LERIELPEYT DEIYHGYLPD AHPGLIYGYR VYGPYDPANG HRFNPNKLLI DPYAKQLVGK
     LKWSEALFGY TIGHPDADLS FDERDSAPFV PKCKVIDPAH TWGHDHRVSV PWDKTIIYET
     HVRGFSMRHP AVPENLRGTF AGLMVDDVVE HIRKLGVSTV ELLPIHAFVN DQHLLHKGMT
     NYWGYNSIAF FAPDPRYLAS GKIAEFKEMV AHLHEANLEV ILDVVYNHTA EGNEQGPTLS
     MRGIDNASYY RLKPDDKRFY INDSGTGNTL DLSHPCVLQM VTDSLRYWAS EMHVDGFRFD
     LATILGRYHD GFDERHSFLV ACRQDPVLRQ VKLIAEPWDC GPGGYQVGNF PPGWVEWNDK
     FRDTVRAFWK GDDGQLADFA SRLTASGEMF NQRGRRPYSS VNFVTAHDGF TLNDLVSYND
     KHNEANDENN QDGSNNNLSW NHGVEGPTDD PEINALRHRQ MRNFFATLLL AQGTPMLVAG
     DEFARTQDGN NNAYCQDSEI GWVNWDLSED GKALLKFVKR LIKLRLTYPV LRRGRFLVGN
     YNEDIGVKDV TWLAPDGTQM TTEHWHDAHN RCLGMLLDGR AQETGIRRKG VDATLMLVVN
     AHHDIVNFRL PEVPDGGFWT CMIDTNQPAI RGQERFEFGH EYSVTGRSLL LFELQHEEQE
//