ID J3HEC4_9PSED Unreviewed; 714 AA.
AC J3HEC4;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 24-JAN-2024, entry version 36.
DE SubName: Full=Isocitrate dehydrogenase, NADP-dependent, monomeric type {ECO:0000313|EMBL:EJM92950.1};
DE Flags: Fragment;
GN ORFNames=PMI33_00520 {ECO:0000313|EMBL:EJM92950.1};
OS Pseudomonas sp. GM67.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=1144335 {ECO:0000313|EMBL:EJM92950.1, ECO:0000313|Proteomes:UP000007281};
RN [1] {ECO:0000313|EMBL:EJM92950.1, ECO:0000313|Proteomes:UP000007281}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GM67 {ECO:0000313|EMBL:EJM92950.1,
RC ECO:0000313|Proteomes:UP000007281};
RX PubMed=23045501; DOI=10.1128/JB.01243-12;
RA Brown S.D., Utturkar S.M., Klingeman D.M., Johnson C.M., Martin S.L.,
RA Land M.L., Lu T.Y., Schadt C.W., Doktycz M.J., Pelletier D.A.;
RT "Twenty-one genome sequences from Pseudomonas species and 19 genome
RT sequences from diverse bacteria isolated from the rhizosphere and
RT endosphere of Populus deltoides.";
RL J. Bacteriol. 194:5991-5993(2012).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR009407-3};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|PIRSR:PIRSR009407-3};
CC Note=Binds 1 Mg(2+) or Mn(2+) ion per subunit.
CC {ECO:0000256|PIRSR:PIRSR009407-3};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EJM92950.1}.
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DR EMBL; AKJH01000015; EJM92950.1; -; Genomic_DNA.
DR RefSeq; WP_008036708.1; NZ_AKJH01000015.1.
DR AlphaFoldDB; J3HEC4; -.
DR OrthoDB; 9807643at2; -.
DR Proteomes; UP000007281; Unassembled WGS sequence.
DR GO; GO:0004450; F:isocitrate dehydrogenase (NADP+) activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR Gene3D; 3.40.718.10; Isopropylmalate Dehydrogenase; 2.
DR InterPro; IPR004436; Isocitrate_DH_NADP_mono.
DR NCBIfam; TIGR00178; monomer_idh; 1.
DR PANTHER; PTHR36999; ISOCITRATE DEHYDROGENASE [NADP]; 1.
DR PANTHER; PTHR36999:SF1; ISOCITRATE DEHYDROGENASE [NADP]; 1.
DR Pfam; PF03971; IDH; 1.
DR PIRSF; PIRSF009407; IDH_monmr; 1.
DR SUPFAM; SSF53659; Isocitrate/Isopropylmalate dehydrogenase-like; 1.
PE 4: Predicted;
KW Magnesium {ECO:0000256|PIRSR:PIRSR009407-3};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR009407-3};
KW NADP {ECO:0000256|PIRSR:PIRSR009407-4}.
FT BINDING 82..87
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-4"
FT BINDING 132..139
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-2"
FT BINDING 135
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-4"
FT BINDING 145
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-2"
FT BINDING 350
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-3"
FT BINDING 547
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-2"
FT BINDING 548
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-3"
FT BINDING 552
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-3"
FT BINDING 584..585
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-4"
FT BINDING 589
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-4"
FT BINDING 600..602
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-4"
FT BINDING 649
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-4"
FT SITE 255
FT /note="Critical for catalysis"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-1"
FT SITE 420
FT /note="Critical for catalysis"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-1"
FT NON_TER 714
FT /evidence="ECO:0000313|EMBL:EJM92950.1"
SQ SEQUENCE 714 AA; 76975 MW; 19346BF260D4F2EF CRC64;
MPTRSKIIYT FTDEAPALAT YSLLPIVEAF TASADIAVET RDISLAGRIL ASFPEQLGDK
AVADHLAELG DLAVTPEANI IKLPNISASV PQLQAAIKEL QAQGFALPDY PETVTSDADK
EAKSRYDKIK GSAVNPVLRE GNSDRRAPLS VKNYARKHPH KMGAWAADSK SHVAHMSTGD
FYGSEKAALI DAADAVKIEL IAQDGTTTVL KEKTTVQAGE ILDCAVMSKN ALRSFIAAEI
EDAKAKGVLL SVHLKATMMK VSDPIMFGQI VAEFYKDALA KHADVLAQIG FNLNNGIGDL
YARIKALPAE QQAQIEADIQ AVYAVRPSLA MVNSDKGITN LHVPSDVIVD ASMPAMIRDS
GKMWGTDGQL HDTKAVIPDR CYATIYQAVI EDCKLHGAFD PTTMGSVPNV GLMAKKAEEY
GSHDKTFQIK TNGVVRVTDS KGALLLEQSV EAGDIFRMCQ TKDAPIQDWV KLAVNRARAS
STPAIFWLDP MRAHDGVVIE KVQAYLKDHD TSGLDIRIMS PVDAMAFTLG RTREGKDTIS
VTGNVLRDYL TDLFPIMELG TSAKMLSIVP LMNGGGLFET GAGGSAPKHV QQLLEENFLR
WDSLGEFLAL AASLEHLGVT YNNPKALVLA KTLDQATGQF LDNNKSPSRK VGNIDNRGSH
FYLALYWAQA LAAQTEDAAL QAQFGELAKT LTENEATIVA ELNAVQGKPV DIGG
//
