ID   J9ITG9_9SPIT            Unreviewed;       700 AA.
AC   J9ITG9;
DT   31-OCT-2012, integrated into UniProtKB/TrEMBL.
DT   31-OCT-2012, sequence version 1.
DT   27-MAR-2024, entry version 33.
DE   SubName: Full=Calpain family cysteine protease containing protein {ECO:0000313|EMBL:EJY75675.1};
GN   ORFNames=OXYTRI_02934 {ECO:0000313|EMBL:EJY75675.1};
OS   Oxytricha trifallax.
OC   Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata; Spirotrichea;
OC   Stichotrichia; Sporadotrichida; Oxytrichidae; Oxytrichinae; Oxytricha.
OX   NCBI_TaxID=1172189 {ECO:0000313|EMBL:EJY75675.1};
RN   [1] {ECO:0000313|EMBL:EJY75675.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JRB310 {ECO:0000313|EMBL:EJY75675.1};
RX   PubMed=23382650; DOI=10.1371/journal.pbio.1001473;
RA   Swart E.C., Bracht J.R., Magrini V., Minx P., Chen X., Zhou Y.,
RA   Khurana J.S., Goldman A.D., Nowacki M., Schotanus K., Jung S., Fulton R.S.,
RA   Ly A., McGrath S., Haub K., Wiggins J.L., Storton D., Matese J.C.,
RA   Parsons L., Chang W.J., Bowen M.S., Stover N.A., Jones T.A., Eddy S.R.,
RA   Herrick G.A., Doak T.G., Wilson R.K., Mardis E.R., Landweber L.F.;
RT   "The Oxytricha trifallax Macronuclear Genome: A Complex Eukaryotic Genome
RT   with 16,000 Tiny Chromosomes.";
RL   PLoS Biol. 11:E1001473-E1001473(2013).
CC   -!- SIMILARITY: Belongs to the peptidase C2 family.
CC       {ECO:0000256|ARBA:ARBA00007623}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:EJY75675.1}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AMCR01012128; EJY75675.1; -; Genomic_DNA.
DR   AlphaFoldDB; J9ITG9; -.
DR   EnsemblProtists; EJY75675; EJY75675; OXYTRI_02934.
DR   OrthoDB; 142935at2759; -.
DR   GO; GO:0004198; F:calcium-dependent cysteine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd00044; CysPc; 1.
DR   Gene3D; 3.90.70.10; Cysteine proteinases; 1.
DR   InterPro; IPR022684; Calpain_cysteine_protease.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   InterPro; IPR001300; Peptidase_C2_calpain_cat.
DR   PANTHER; PTHR10183; CALPAIN; 1.
DR   PANTHER; PTHR10183:SF420; FAMILY CYSTEINE PROTEASE, PUTATIVE-RELATED; 1.
DR   Pfam; PF00648; Peptidase_C2; 1.
DR   PRINTS; PR00704; CALPAIN.
DR   SMART; SM00230; CysPc; 1.
DR   SUPFAM; SSF54001; Cysteine proteinases; 1.
DR   PROSITE; PS50203; CALPAIN_CAT; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|PROSITE-ProRule:PRU00239};
KW   Protease {ECO:0000256|PROSITE-ProRule:PRU00239,
KW   ECO:0000313|EMBL:EJY75675.1};
KW   Thiol protease {ECO:0000256|PROSITE-ProRule:PRU00239}.
FT   DOMAIN          42..342
FT                   /note="Calpain catalytic"
FT                   /evidence="ECO:0000259|PROSITE:PS50203"
FT   ACT_SITE        111
FT                   /evidence="ECO:0000256|PIRSR:PIRSR622684-1,
FT                   ECO:0000256|PROSITE-ProRule:PRU00239"
FT   ACT_SITE        261
FT                   /evidence="ECO:0000256|PIRSR:PIRSR622684-1,
FT                   ECO:0000256|PROSITE-ProRule:PRU00239"
FT   ACT_SITE        286
FT                   /evidence="ECO:0000256|PIRSR:PIRSR622684-1,
FT                   ECO:0000256|PROSITE-ProRule:PRU00239"
SQ   SEQUENCE   700 AA;  80353 MW;  B581881DB4A24794 CRC64;
     MNLTSLNIGE LGRQIFARLL KSTASDGTLD DILYECEQQN EPYCDEEFPA SNQSLVKNWR
     DPSCQDKQNW KQFKWLRPQE ITSIKTSGKE IGIFVDSIDP SDINQGLLGD CYFLSCLSVL
     AENPQRIRRL FVTDQINEYG VYGVRICKNG EWREIVIDDR IPCQRGEPAF SKAIGNELWV
     PLMEKAWAKL HGSYDRVEAG FAENALRDLT GAPCEVIDSD EPNLWSKLME AEKKGFLIAA
     SAGKTDASKE LLKNLGLVGS HSYGVLDVRE VTLDNGRQEK LIKIRNPWGN FEWNGDWGDE
     SKLWTPSLEK QVGLQKQNDG IFFMNMKDFC HYFGRVQICR IDDNFKHSSF KCNQSSGSYC
     LIKFVVQTDG LYNLSLLQQD ERCFDRSDNY DYSIAKFILA KITSQNESKL ELEYIGGILG
     KERDMYKEYQ MLKKGEYYIF AEIDWVDGAF TSEFTLSSYG TGQTFFLRDD SQLYSKQEIL
     SQIFLNCALQ QLPDCNYSNY EKDQEPNIHK YYQVCKEGYR YIVYNNLSQD STLVECVTYL
     VFDGLELVKP YKGQSFEITL KPKEKKIVLI KDVNPFSNSI RTQIQQKICF STQKLLKLCK
     EKGEMSQRED SRSKQPVDIY QYELEHPNGI CYLYENKTSN KTLEENIKFT INGLEIVEKP
     ADGSNTVKVK LGPGESKLIE LQKTSLNGYS ISAGISYLVK
//