ID J9J6L2_9SPIT Unreviewed; 845 AA.
AC J9J6L2;
DT 31-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 31-OCT-2012, sequence version 1.
DT 27-MAR-2024, entry version 47.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=OXYTRI_16720 {ECO:0000313|EMBL:EJY85418.1};
OS Oxytricha trifallax.
OC Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata; Spirotrichea;
OC Stichotrichia; Sporadotrichida; Oxytrichidae; Oxytrichinae; Oxytricha.
OX NCBI_TaxID=1172189 {ECO:0000313|EMBL:EJY85418.1};
RN [1] {ECO:0000313|EMBL:EJY85418.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JRB310 {ECO:0000313|EMBL:EJY85418.1};
RX PubMed=23382650; DOI=10.1371/journal.pbio.1001473;
RA Swart E.C., Bracht J.R., Magrini V., Minx P., Chen X., Zhou Y.,
RA Khurana J.S., Goldman A.D., Nowacki M., Schotanus K., Jung S., Fulton R.S.,
RA Ly A., McGrath S., Haub K., Wiggins J.L., Storton D., Matese J.C.,
RA Parsons L., Chang W.J., Bowen M.S., Stover N.A., Jones T.A., Eddy S.R.,
RA Herrick G.A., Doak T.G., Wilson R.K., Mardis E.R., Landweber L.F.;
RT "The Oxytricha trifallax Macronuclear Genome: A Complex Eukaryotic Genome
RT with 16,000 Tiny Chromosomes.";
RL PLoS Biol. 11:E1001473-E1001473(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EJY85418.1}.
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DR EMBL; AMCR01003122; EJY85418.1; -; Genomic_DNA.
DR AlphaFoldDB; J9J6L2; -.
DR EnsemblProtists; EJY85418; EJY85418; OXYTRI_16720.
DR OrthoDB; 5475708at2759; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 45..362
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 363..765
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 1..39
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 582..609
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 621..653
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 66..73
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 845 AA; 98508 MW; 22B2E65CD763A8CA CRC64;
MKKVSKDKTI EINPQKRSNS KSDSKKSKKS KEEQKHILDK YPQFQELNDD QKEAVVYNDS
PLLILAGVGT GKTQTLMSKY AYLVMEGQQK FDINQTNILA VTFTNKAAKT MQVRASQITQ
RDLRQNWIGT FHSLSARMLL QDDHYKLVQL KEDYHLLSQE KQRKQVRKVL KDQNAIRDLI
LNHEQRQLDK QQQKGQMSQY SQYYRQQKPS MPYSKILRAI DTFKNRLLKP DDQIPDDLKE
FEQIVFYNVY ELYQESMRKE NLIDFGDLLM YVVDILKRFP EILQKFRAQF KYILVDEVQD
LNYIQQEWLK LIVGDNLHLT CVGDDDQMVY GFRGASGDFI LNFEHHFDTS HTIKLEQNYR
STKKILEGAN NLISKNFNRK GKTLWTDNQT GEDISFLEYK SKDQEIQSIV EYIKQMIGGG
EDFGFSPEQI AILTRTNADG REIEKCLIKE SVNYELAANK KAFLDLKEVN LALMFLVILH
KPFDNYAWKE ILGNLEGFGK DTVKKITTYA EKNDIAIHDI CEYLVEPCRY NLGKRIEAPT
NVRTLESRIK SSVLGNNFSN AQSKVGNIQN LKQNSLSSYY SIQKQKQKEE KKTRYSKGPA
KNKKGNIKNL NCDDSDSWEC INDQPIDKND QSIMSDKSDQ SVQTPPQPQS KPLYTTIDID
HKLTKKQREK LEALISARDK YKKGKTNNFA SYLEDSLFYD CLSKISKTKE GKSNKVQENV
RDLLEIALTF EDIDEFFNHI TLFSGDASKQ DQKAIKVMTI HASKGLEFNQ VFLPFWVKGK
FPVELSRENE KVEVEEERRT AFVGLTRARE HLFISHHEEC EEFVKQQQVS QFVQEIQTKT
YKIKK
//
