ID K0K6B9_WICCF Unreviewed; 1409 AA.
AC K0K6B9;
DT 28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT 28-NOV-2012, sequence version 1.
DT 27-MAR-2024, entry version 55.
DE RecName: Full=DNA polymerase {ECO:0000256|RuleBase:RU000442};
DE EC=2.7.7.7 {ECO:0000256|RuleBase:RU000442};
GN Name=REV3 {ECO:0000313|EMBL:CCH40490.1};
GN ORFNames=BN7_23 {ECO:0000313|EMBL:CCH40490.1};
OS Wickerhamomyces ciferrii (strain ATCC 14091 / BCRC 22168 / CBS 111 / JCM
OS 3599 / NBRC 0793 / NRRL Y-1031 F-60-10) (Yeast) (Pichia ciferrii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Phaffomycetaceae; Wickerhamomyces.
OX NCBI_TaxID=1206466 {ECO:0000313|EMBL:CCH40490.1, ECO:0000313|Proteomes:UP000009328};
RN [1] {ECO:0000313|EMBL:CCH40490.1, ECO:0000313|Proteomes:UP000009328}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 14091 / BCRC 22168 / CBS 111 / JCM 3599 / NBRC 0793 / NRRL
RC Y-1031 F-60-10 {ECO:0000313|Proteomes:UP000009328};
RX PubMed=23193139; DOI=10.1128/EC.00258-12;
RA Schneider J., Andrea H., Blom J., Jaenicke S., Ruckert C., Schorsch C.,
RA Szczepanowski R., Farwick M., Goesmann A., Puhler A., Schaffer S.,
RA Tauch A., Kohler T., Brinkrolf K.;
RT "Draft genome sequence of Wickerhamomyces ciferrii NRRL Y-1031 F-60-10.";
RL Eukaryot. Cell 11:1582-1583(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|RuleBase:RU000442};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966,
CC ECO:0000256|RuleBase:RU000442};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|RuleBase:RU000442}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-B family.
CC {ECO:0000256|ARBA:ARBA00005755, ECO:0000256|RuleBase:RU000442}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCH40490.1}.
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DR EMBL; CAIF01000001; CCH40490.1; -; Genomic_DNA.
DR STRING; 1206466.K0K6B9; -.
DR eggNOG; KOG0968; Eukaryota.
DR HOGENOM; CLU_000203_3_1_1; -.
DR InParanoid; K0K6B9; -.
DR Proteomes; UP000009328; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0016035; C:zeta DNA polymerase complex; IEA:InterPro.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0019985; P:translesion synthesis; IEA:InterPro.
DR CDD; cd05778; DNA_polB_zeta_exo; 1.
DR CDD; cd05534; POLBc_zeta; 1.
DR Gene3D; 1.10.132.60; DNA polymerase family B, C-terminal domain; 1.
DR Gene3D; 3.30.342.10; DNA Polymerase, chain B, domain 1; 1.
DR Gene3D; 1.10.287.690; Helix hairpin bin; 1.
DR Gene3D; 3.90.1600.10; Palm domain of DNA polymerase; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR042087; DNA_pol_B_thumb.
DR InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR InterPro; IPR030559; PolZ_Rev3.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR025687; Znf-C4pol.
DR PANTHER; PTHR45812; DNA POLYMERASE ZETA CATALYTIC SUBUNIT; 1.
DR PANTHER; PTHR45812:SF1; DNA POLYMERASE ZETA CATALYTIC SUBUNIT; 1.
DR Pfam; PF00136; DNA_pol_B; 1.
DR Pfam; PF03104; DNA_pol_B_exo1; 1.
DR Pfam; PF14260; zf-C4pol; 1.
DR PRINTS; PR00106; DNAPOLB.
DR SMART; SM00486; POLBc; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485, ECO:0000256|RuleBase:RU000442};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA replication {ECO:0000256|RuleBase:RU000442};
KW DNA-binding {ECO:0000256|RuleBase:RU000442};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU000442};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|RuleBase:RU000442};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|RuleBase:RU000442};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|RuleBase:RU000442};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU000442};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|RuleBase:RU000442};
KW Reference proteome {ECO:0000313|Proteomes:UP000009328};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU000442};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU000442};
KW Zinc-finger {ECO:0000256|RuleBase:RU000442}.
FT DOMAIN 602..774
FT /note="DNA-directed DNA polymerase family B exonuclease"
FT /evidence="ECO:0000259|Pfam:PF03104"
FT DOMAIN 854..1294
FT /note="DNA-directed DNA polymerase family B
FT multifunctional"
FT /evidence="ECO:0000259|Pfam:PF00136"
FT DOMAIN 1321..1386
FT /note="C4-type zinc-finger of DNA polymerase delta"
FT /evidence="ECO:0000259|Pfam:PF14260"
SQ SEQUENCE 1409 AA; 162870 MW; C09B789E7AF4F444 CRC64;
MQRRNRDDFY ATKSDSFVED DFKIQINTYD HYQVTPSIFD RDGPINKEQE FSFHHVPVVR
VFGSIPTGHN IIAHIHGAYP YIYIPYDNQD CFRLQQVLEA AIASSLNRKK SDNENTNDNH
KESKVFKYIA HISLCKGVPF YGYNVGYKAF YKIYLLNPSY SNRLAEMLRD GKATGKKYDC
YEVHIPYVLQ FLSDFNLFGC AWLRLKNLYL RSPILSDDSL KTDELVEYLT GFQPTDAERT
GYSALEIDVC AQDILNRLDL KERELHHDFI EKFNPPSSDI YIQSTKDLWK DGDFQRKAKG
EVEYSTPQST LRSPNVEWVE TEELNQLFEY AKKINGETTA DFSNFIKKGS WENDLKSSFQ
TVSEMFHEPD LYTDHEKTIQ SEHSGEEDEY DPNDFLLDDY QGPAVMNRDD DLEIFENARD
DDERNAVPGI YEPEDPNDFE GSDEFFLSES QEKLQNAPDI SDIHLTQNMN VKFTQNNDLS
RVLSSLDSST KVKNKFPKLA ESRFIYSKAP PLDLDFGAVG LPQIDYNDPF YTEEKPKPFV
FGGKKFNLKS KAPKDLDGLE VHGESISKEI EKGFVLQENT FKSWRYIKKP PSFNQVKNFV
YGSQIKSQIG VTQRHKFASP QTNERRPDGS NHMTVLIVEV HSNTRESLRP DPKYDEVSAI
FWKTMNGSTH REGVYLLNVK QPVPDKEVTH FISELEMFES FTTFVRKFDP DIISGFEVQS
GSWGYLIERA RAIYEFDFVP ELSRVHFKAH NKMGDHWGYT HTSSIKVCGR QVLNIWRPLR
RLNLLKYTIE NVAYHALHER LPHYSNKTLT KMFQDDFLSL SSHYFKKLEI DGRLIDTQEL
ITRAAEEARL IGIDFNDVYY RGSQYKVESF MVRMAKAENF MLFSPSKRQV RKQKPLECIP
LVMEPISAYY KSPLIVLDFQ SLYPSIVIAY NYCYSTLLGR LRNYSNGPNH IGTSHVKHPP
GLLKLLENDI NISPNGLMFA KSSVRKSLLA KMLEEILDTR FMVKSTMKFL DENMKQLYNS
RQLALKLIAN VTYGYTSASF SGRMPCSDIA DAIVQTGRET LDKAVDIIEG QSSWGAKVVY
GDTDSLFVYL PGRSRDDAFR IGKEMADAVT KSNPDPVTLK FEKVYHPCVL MAKKRYVGYS
YENINSPVKF DAKGIETVRR DGHPAQQHIV EKCLRILFET NDISQIKNFV QDQFNKITYN
KVSIQDFCFA KEVKIGTYKQ PPPGAVVSTK LMEQDSRAEP QYKERVPYVI IQETGSILRE
RARTPEQFIE NNYKLDAEYY IVKTLIPPLE RIFNLLGVDV KPWYNELPRP AKRSSIYNSI
CLSCNVPTKS NLCQSCRNDE LKTVLNLNQR MKQIEGRMKD LMTVCRSCSG HSVSCESHDC
PVYYTRVKEG KKLEAAVQDR KRLKKEIDW
//
