ID K0K907_WICCF Unreviewed; 4944 AA.
AC K0K907;
DT 28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT 28-NOV-2012, sequence version 1.
DT 27-MAR-2024, entry version 54.
DE RecName: Full=Midasin {ECO:0000256|ARBA:ARBA00017143, ECO:0000256|PIRNR:PIRNR010340};
GN Name=MDN2 {ECO:0000313|EMBL:CCH41350.1};
GN ORFNames=BN7_889 {ECO:0000313|EMBL:CCH41350.1};
OS Wickerhamomyces ciferrii (strain ATCC 14091 / BCRC 22168 / CBS 111 / JCM
OS 3599 / NBRC 0793 / NRRL Y-1031 F-60-10) (Yeast) (Pichia ciferrii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Phaffomycetaceae; Wickerhamomyces.
OX NCBI_TaxID=1206466 {ECO:0000313|EMBL:CCH41350.1, ECO:0000313|Proteomes:UP000009328};
RN [1] {ECO:0000313|EMBL:CCH41350.1, ECO:0000313|Proteomes:UP000009328}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 14091 / BCRC 22168 / CBS 111 / JCM 3599 / NBRC 0793 / NRRL
RC Y-1031 F-60-10 {ECO:0000313|Proteomes:UP000009328};
RX PubMed=23193139; DOI=10.1128/EC.00258-12;
RA Schneider J., Andrea H., Blom J., Jaenicke S., Ruckert C., Schorsch C.,
RA Szczepanowski R., Farwick M., Goesmann A., Puhler A., Schaffer S.,
RA Tauch A., Kohler T., Brinkrolf K.;
RT "Draft genome sequence of Wickerhamomyces ciferrii NRRL Y-1031 F-60-10.";
RL Eukaryot. Cell 11:1582-1583(2012).
CC -!- FUNCTION: Nuclear chaperone required for maturation and nuclear export
CC of pre-60S ribosome subunits. {ECO:0000256|PIRNR:PIRNR010340}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000256|ARBA:ARBA00004604}. Nucleus, nucleoplasm
CC {ECO:0000256|ARBA:ARBA00004642}.
CC -!- SIMILARITY: Belongs to the midasin family.
CC {ECO:0000256|ARBA:ARBA00007188, ECO:0000256|PIRNR:PIRNR010340}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCH41350.1}.
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DR EMBL; CAIF01000017; CCH41350.1; -; Genomic_DNA.
DR STRING; 1206466.K0K907; -.
DR eggNOG; KOG1808; Eukaryota.
DR HOGENOM; CLU_000050_0_2_1; -.
DR InParanoid; K0K907; -.
DR Proteomes; UP000009328; Unassembled WGS sequence.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR CDD; cd00009; AAA; 2.
DR CDD; cd01460; vWA_midasin; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 7.
DR Gene3D; 3.40.50.410; von Willebrand factor, type A domain; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR040848; AAA_lid_7.
DR InterPro; IPR011704; ATPase_dyneun-rel_AAA.
DR InterPro; IPR048617; MDN1_AAA_lid_4.
DR InterPro; IPR012099; Midasin.
DR InterPro; IPR041190; Midasin_AAA_lid_5.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR InterPro; IPR002035; VWF_A.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR48103:SF2; MIDASIN; 1.
DR PANTHER; PTHR48103; MIDASIN-RELATED; 1.
DR Pfam; PF07728; AAA_5; 7.
DR Pfam; PF17865; AAA_lid_5; 1.
DR Pfam; PF17867; AAA_lid_7; 3.
DR Pfam; PF21108; MDN1_4th; 1.
DR PIRSF; PIRSF010340; Midasin; 1.
DR SMART; SM00382; AAA; 6.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 6.
DR SUPFAM; SSF53300; vWA-like; 1.
DR PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR PROSITE; PS50234; VWFA; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PIRNR:PIRNR010340};
KW Chaperone {ECO:0000256|ARBA:ARBA00023186, ECO:0000256|PIRNR:PIRNR010340};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|PIRNR:PIRNR010340};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|PIRNR:PIRNR010340};
KW Reference proteome {ECO:0000313|Proteomes:UP000009328}.
FT DOMAIN 4738..4933
FT /note="VWFA"
FT /evidence="ECO:0000259|PROSITE:PS50234"
FT REGION 4065..4624
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 2180..2207
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 4096..4110
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4118..4176
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4177..4239
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4240..4265
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4281..4406
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4407..4437
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4475..4532
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4552..4595
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4596..4619
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 4944 AA; 560660 MW; 9BE7D4486D2847DB CRC64;
MSDTIIFNYS EAQDRYDQFK KFYNRKQLPQ QLFQFDTTKT INENLRQLAL VSLEPTITSA
YLYSFDQIFL DLVSRWISDE SIEIEYNQLN KQSNIINGTV ILSSLAKIIS LSPNITSLVE
YYLENLNIFQ NLETNQSKIS NLELQQILLA FHRLLYHDQQ RFIRFISPHT LTNILKFTED
EVVKFLIIQI LSKYLNLTEK AKNDLLNNHI TKPIIGSYES DKNIDYQFLS VLEAQRLANH
HNIQSQSSSS NSEPSNSDIF INIENTSISS LVVSVCGVLV SKIHLDITTK TQSSLVPTER
SIDTLRKLAL NVQSSSPVML VGNAGSGKTF LINELSKYLS CDKTIVKIHL GEQTDAKLLL
GTYTSGQKPG TFEWRSGVLT TAVKEGRWVL IEDIDKAPTE VLSILLSLLE KRELTIPSRG
EVIKAANGFQ LISTIRTTNN YKHNKSSTIP EIIGLRLWNV LQLNDQSDDE LLQILTSKFP
ILSKLIPNFI KLYESIQLAY NGKQFLSLNR GSQPRIISLR DLMKFCQRVD VLFQNHGVNS
SSQLLESSVL DNIFAEAVDC FAGAISEYSA LEVLINTIGE ALQISSSRIN LFISKHVPLY
EDFPEKLIIG RAHLSKAPSS LYKKKNTGNN TSFARTNHSL KLMEQIGVSV QMTEPVLLVG
ETGTGKTTVV QQVAKQLNKE LTVINVSQQT EAGDLLGGYK PVNTKSVAVP IQEEFENLFH
LTFSMKKNEK FHKMLTKCFN KNQWKNVIRL WQEAIKLSEK VLNTPDEDFN DEAPKKKRRL
NTHDKKLLIE KWNEFTQNVK NFEVQSSSLE NSFVFNFVEG SLVKAVRNGD WLLLDEINLA
SPDTLESISD LLSDTSGRRS ILLSEKGEVE SIQAHKDFRI FACMNPATDV GKRDLPLSIR
SRFTEVYVHS PDGDISDLLS IIDKYLSRFA LNDEWIGNDV AQLYLEAKKL ADSNQIVDGA
NQKPHFSIRT LTRTLLYVVD IVSIYGLRRS LYEGFCMSFL TLLDLKSEEI LKPLIEKYTI
HRLKNAKSVI SQIPPAPSSE SGSFVQFRHY WMRQGPSEII DQPHYIITPF VEKNMMNLVR
ATSGRKFPIL VQGPTSSGKT SMIKYLADIT GHKFVRINNH EHTDLQEYLG TYISDDTGRL
SFKEGVLVEA LRNGHWLVLD ELNLAPTDVL EALNRLLDDN RELFIPETQE VVRPHPDFML
FATQNPPGLY GGRKVLSRAF RNRFLELHFD DIPQDELEVI LRDRCQIAPS YAKKIVEVYR
QLSVQRQSSR LFEQKNSFAT LRDLFRWALR EAVGYEQLAA NGYMLLAERV RKPEEKKVVQ
EVLEKVMRVK LDMNAYYNSL ENQDLMNTPS QVVWTKAIRR LSVLISTALQ NNEPILLVGE
TGCGKTTVCQ LLAQFNNKKL ITVNAHQNTE TGDLLGAQRP VRNKSELQEN LTKKVIEALQ
SIGVTPGGDD LRELLQFYDN QDLTELNEDI RKDIQHDRSN LNVLFEWSDG PLIQALNSGS
YFLLDEISLA DDSVLERLNS VLEPERTLLL AEKGTEDSSI TAKDGFQFLA TMNPGGDYGK
KELSPALRNR FTEIWVPSME DFGDVKTIVE EKLDENVKYL ADGIVKFSER FGMKLGGGNI
TSGVISLRDI LAWVEFINST QNTGIPINSC LLNGASLVFI DALGTNNTAY LAESEEKLLQ
MKLDCVKELC EIAGDDLTQY YRQKVDISLN PSTLNAGGFE IARDSTNSKD EFFSFEAPTT
AANTMRVVRA MQVKKPILLE GSPGVGKTSL VSALAKATGN QLTRINLSEQ TDLVDLFGSD
APAEGGKTGE FVWRDAPFLR AMQRGEWVLL DEMNLASQSV LEGLNACLDH RGEAYIPELD
RSFSRHPNFV VFAAQNPQYQ GGGRKGLPKS FVNRFTVVYV DTLTSEDLVL IAHHLYPNIN
ADTCSKMIGL MSRLDHEVCV TKSWGASGSP WEFNLRDTLR WLELYNAEYL SSGLSPIDFL
NIIVTQRFRT VSDREKASKL VHETFEEEPC TDNYFTITES YFQAGKALMS RGSSVQETSH
TRLNHLQSNT EFVESSLLAI QNNWPLIFVG SSSSGKTELV RYLATISSAK VVEFSMNSDI
DSMDILGGYE QVDLTKRVTA IVEKFLRILN VGIADQLKNS SELLNTTIAS LEFVKFLKTV
HITIENFSLL VEHFGRFTEL VRDNDQINEV EKELINLKEL LKQSSSVKFE WFDGLLVKAV
ERGDWLILDN ANLCSPSVLD RLNSLLEING SLIMNECSMA DGQARVLKPH PNFRLFLTVD
PKFGELSRAM RNRGVEIYLD KLDHRATTFD RNVLGFKAKK SKKEELDLNE LSLNENEPIA
PIQKFIESTD SFSRSLSLVI DAIMTSGKEF MLNHLAGILP FLNQLSIERF TNTIYSSTEV
TKEVIDALSE ISSFVDYVTQ LGLTKSIIGK FEDCENVVDS LLGQKSNFSI NQSLNPGWNY
YAIKRIQSNS LIPLNEAISL IQVASDLSQI EKALQSAEVR GANGKLNELS FLEQGAALSS
GRNMKNPPKV NVFNFLKEIY EFILGSFKNK DTLSLLSQSS CFEALLDLTR IWKSVWDISD
VKNEAKLRVY QKIINEWIEG ATEFEGIDIL KNIMKVYGNA LKSDKGEYMS AIWESFRGVY
PQSEQAWADN DVLSSLAEKF DAVSHEQFSE SDNHILTLKS AILNIKNVIL ANDGSDISNA
FTLLENGIRD LHEVSSKFLV KRAHNFEESF YLLFNMVLSS GSVKNEDIIA IAHQANISTT
CLLKNTNTDS YPPVFNTLWA KTNNGYVSFV SHLFTNKFLH HIFINSQSYL TFSGNQVNQT
VNDSVNFTKY LIKYSDEVSR DTHKEQVAII LNWYQKIVRI VSSDDSLSVW DGNSFDKNDL
PQLSEKVAGI SANGFTAINK NFFQPALELI TGSVNIEKLG KAWVLFSIGL IQLYVPDNAN
DPAIEDHILY ENYEKKKSLF TQLSNSVKNI RSVYFGDDEI VIEHHLPTVD EESKPSKPRV
YRSKVPIDNL FEEWKIFMSS TLDNNSLVKF LATVEETELE TLSGELNMFQ QNSSNFIERL
ENQFSHFSDV NDIFKGYIYG IKLGFDLILE AKKKTKVTYN MDPLWFISFQ NITNDKEIEK
GLATVRNFCK NMDVDNTLAE QVMHITLKLA SSSEQGINHQ ILEQVFQTIY YRWSLRRTRQ
EKKDAIEGSV FRYRGEEDED EDFKKIFPDY DEYVELDTDS KSRSDSSRLD EEYANICKTY
IDVFTKDKAY ALKDLIVNGG DIAKKLISLS DEFETGVIDP TSLGSVISDL FRSIDSATIE
KAGKDIDFYF GYSPPELRRS IKVVEKLQYS VHGLLQQWPE HATLQTLFRI SQEYLAFPVK
TPIARLIQKI EQIYTFIIEW EKYASVKVSL SAHQDDFTSL IVSWRRMELS SWKSLFKNEE
ISNQRSIGGF WFNLYENLIL PSYSGVQIDI GQLVLILNSF ISKSSYGQYE LRLNLLKAIK
THLSSISSDK LLLNMLKNLI KYYEQFSPII HENINRVKKT LEKDVEEVIL LASWKDVNID
ALKQSARRSH QSLYKVVRKY RTLLATEVTP LIEQGLPDSQ KVSVNKDSVL VSKLELNADI
GDDHKTIDGW DTRSQNLKQT ETVQKNMNTY VSRVKNTEFP ELYEFASDIV KESDRLRDDT
PKTYSEENKK EVAALKTQKM KLLSDSIKEL RRIGLKSRIR EDIHKLQVTA NQVLSGAEAF
DNTQLEGSDA YFFRIVDILP RLRASVSSVA EDVPAAAAEN GLAIIENLVY AVINDKKLLA
KVAHKLNDFE SDKSEFQKLI DSENLYVGKS TSALGLVKFN KICRWLPEIL SLSMNLAATS
SKMLQLSVDL SIFDSFVKKV SEFKEKIISQ LKYSSIVDSQ LAEFDNILKS FVSDLESWKS
SNKSIGFVSD SVINWLNSLN DVEIEDSENQ DTTVAEIEKT LRSFSNKVLV VFQKVIQSLS
SEELTFDTDK WFIQSEAQLH TLLKSLHHES VHSSLHKALE LVSSAKFDQA QSSQIRSLFS
FTAPFLKFYL ELVVAVLNKA KDSYIDISRS TYVFSNLLHN LTKNGFCSPE PPSETKQDEN
LHDGTGLGDG EGAQNNSNDV EDDEDLSEQA QEPNKDEKDD KDDEDDNQDA VDIEGDMAGD
LENASDQDQD DDEEQDKDEE QDELDEEVDD LDDLDPNAVD DKMWDEEVQE DKKEKESENM
PENSTADDMK AQEDEPEEKP EDKESKEDDK SSKDQENDDD KKEDDQDAED EEDVGEQEDD
VKNEEKEDLE QNVPEGEALD LPDDINLDSG EEDEEDDKQD DGDEGMDDGL DDKMDLDEDE
AENGEEENVN DAEENQEEQE GEEGEEQESE EANQAGDEND QEEENQDQND EEQDAIEAGE
KSEDEEELKD AKNEEDQQDE AAEGEENGDR MEGIDSGETG DNNNEDIDKE TATEEKSGDQ
GDGAQAQAED EKEDVGASGS AMNQQQEDND NDGNNDEARD NAQESLKQLG DSLKEFHRRH
QDIREVSEKD ETAEQAANER PDEFEHVDGE NTEETTQALG AANKDQISSI DEDKAIDDDE
DDQDEEIKNE DQDENMVEGE EANEDAEEIE NQDDEKPDEF NGKTKGGFVG ERKTENLDDM
DDMDDINMDQ NLDELDSDSD EDFSLLKPEV IDDDEDHRDI EEARELWRKS DLETQELSAN
LCEQLRLILE PTLSTKLKGD YKTGKRLNMK RIIPYIASEF RKDKIWLRRT KPSKRQYQIM
IAVDDSKSMS ESKSVDLAFQ SIALVSKALT QLESGGLSIV KFGENTKVVH PFEKQFSPDT
GAKIFQKFDF QQTRTDIKKL VGESIEIFNN AKSLGNSDLW QLQIIISDGV CEDHDTVQRL
VRRAREERIM LVFVIIDGIN SNESIMDMSQ VSYVPDANGN MVLKVDKYLD SFPFEFYVVV
HDITDLPKML SLILRQYFSE LASN
//
